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Age-Dependent Effects of Acute Alcohol Administration in the Hippocampal Phosphoproteome
Chemical Research in Toxicology ( IF 4.1 ) Pub Date : 2017-11-03 00:00:00 , DOI: 10.1021/acs.chemrestox.7b00260
Ana Contreras 1 , Lidia Morales 1 , Ali Tebourbi 1 , Miguel Miguéns 2 , Nuria del Olmo 1 , Carmen Pérez-García 1
Affiliation  

Alcohol consumption during adolescence is deleterious to the developing brain and leads to persistent deficits in adulthood. Several results provide strong evidence for ethanol-associated alterations in glutamatergic signaling and impaired synaptic plasticity in the hippocampus. Protein phosphorylation is a well-known and well-documented mechanism in memory processes, but information on phosphoprotein alterations in hippocampus after ethanol exposure is limited. This study focuses on age-related changes in the hippocampal phosphoproteome after acute alcohol administration. We have compared the phosphoprotein expression in the hippocampus of adult and adolescent Wistar rats treated with a single dose of ethanol (5 g/kg i.p.), using a proteomic approach including phosphoprotein enrichment by immobilized metal affinity chromatography (IMAC). Our proteomic analysis revealed that 13 proteins were differentially affected by age, ethanol administration, or both. Most of these proteins are involved in neuroprotection and are expressed less in young rats treated with ethanol. We conclude that acute alcohol induces important changes in the expression of phosphoproteins in the hippocampus that could increase the risk of neurodegenerative disorders, especially when the alcohol exposure begins in adolescence.

中文翻译:

急性饮酒对海马磷酸化蛋白质组的年龄依赖性。

青春期饮酒对大脑发育有害,并导致成年后持续缺乏。几项结果提供了与乙醇有关的谷氨酸能信号改变和海马突触可塑性受损的有力证据。蛋白质磷酸化是记忆过程中众所周知的机制,但是乙醇暴露后海马磷酸蛋白改变的信息是有限的。这项研究的重点是急性酒精管理后海马磷酸化蛋白质组中与年龄相关的变化。我们使用蛋白质组学方法(包括通过固定金属亲和层析(IMAC)富集磷蛋白),比较了单剂量乙醇(5 g / kg ip)处理的成年和青春期Wistar大鼠海马中磷蛋白的表达。我们的蛋白质组学分析显示13种蛋白质受年龄,乙醇施用或两者同时影响。这些蛋白质大多数都参与神经保护作用,在用乙醇处理的幼鼠中表达较少。我们得出的结论是,急性酒精会诱发海马中磷蛋白表达的重要变化,这可能会增加神经退行性疾病的风险,尤其是在青春期开始暴露酒精时。
更新日期:2017-11-03
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