A thermo-responsive surface was fabricated by depositing poly(N-isopropylacrylamide) (PNIPAAm) onto polydopamine coated cell culture substrata through free radical polymerization for the purpose of culturing and harvesting human mesenchymal stem cells (hMSCs). Human MSCs were cultured onto the PNIPAAm-g-polydopamine coated surface and harvested by changing from physiological to ambient temperature. The produced PNIPAAm-g-polydopamine surface was characterized by atomic force microscopy, Fourier transform infrared spectroscopy, nuclear magnetic resonance, water contact angle measurement, differential scanning calorimetry, and cell culture studies. Our results revealed that hMSCs could be detached from the PNIPAAm-g-polydopamine surface within 60 min after switching the temperature from 37 °C to room temperature. The detached hMSCs were able to proliferate on the PNIPAAm-g-polydopamine coated surface for further growth and harvest.

中文翻译：

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聚（N-异丙基丙烯酰胺）修饰的聚多巴胺作为间质干细胞培养和收获的温度响应性表面

通过自由基聚合将聚（N-异丙基丙烯酰胺）（PNIPAAm）沉积到聚多巴胺涂层的细胞培养基质上来制备热响应表面，以用于培养和收获人间充质干细胞（hMSCs）。人MSC中培养到PNIPAAm-克-polydopamine涂覆的表面，并通过从生理变化至环境温度收获。通过原子力显微镜，傅里叶变换红外光谱，核磁共振，水接触角测量，差示扫描量热法和细胞培养研究对所产生的PNIPAAm- g-聚多巴胺表面进行表征。我们的研究结果表明，可能的hMSCs从PNIPAAm-拆卸g ^-将多巴胺表面温度从37°C切换到室温后60分钟之内。分离的hMSC能够在PNIPAAm- g-聚多巴胺包被的表面上增殖，以进一步生长和收获。