当前位置: X-MOL 学术Biomater. Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Thermo-rheological responsive microcapsules for time-dependent controlled release of human mesenchymal stromal cells
Biomaterials Science ( IF 6.6 ) Pub Date : 2017-09-18 00:00:00 , DOI: 10.1039/c7bm00663b
W. C. Mak 1, 2, 3, 4, 5 , B. Magne 1, 2, 3, 4 , K. Y. Cheung 1, 2, 3, 4 , D. Atanasova 1, 2, 3, 4 , M. Griffith 1, 2, 3, 4, 6
Affiliation  

Human mesenchymal stromal cells (hMSCs) are adult-source cells that have been extensively evaluated for cell-based therapies. hMSCs delivered by intravascular injection have been reported to accumulate at the sites of injury to promote tissue repair and can also be employed as vectors for the delivery of therapeutic genes. However, the full potential of hMSCs remains limited as the cells are lost after injection due to anoikis and the adverse pathologic environment. Encapsulation of cells has been proposed as a means of increasing cell viability. However, controlling the release of therapeutic cells over time to target tissue still remains a challenge today. Here, we report the design and development of thermo-rheological responsive hydrogels that allow for precise, time dependent controlled-release of hMSCs. The encapsulated hMSCs retained good viability from 76% to 87% dependent upon the hydrogel compositions. We demonstrated the design of different blended hydrogel composites with modulated strength (S parameter) and looseness of hydrogel networks (N parameter) to control the release of hMSCs from thermo-responsive hydrogel capsules. We further showed the feasibility for controlled-release of encapsulated hMSCs within 3D matrix scaffolds. We reported for the first time by a systematic analysis that there is a direct correlation between the thermo-rheological properties associated with the degradation of the hydrogel composite and the cell release kinetics. This work therefore provides new insights into the further development of smart carrier systems for stem cell therapy.

中文翻译:

热流变响应微胶囊用于人间充质基质细胞的时间依赖性控制释放

人间充质基质细胞(hMSCs)是成人来源的细胞,已经广泛评估了基于细胞的疗法。据报道,通过血管内注射递送的hMSC在损伤部位蓄积以促进组织修复,并且还可以用作递送治疗基因的载体。然而,由于无神经和不良的病理环境,注射后细胞会丢失,因此hMSC的全部潜力仍然有限。已经提出将细胞包封作为增加细胞生存力的手段。然而,控制治疗细胞随时间释放至靶组织仍是当今的挑战。在这里,我们报告设计和开发的热流变响应水凝胶,允许精确,时间依赖的hMSCs的控制释放。取决于水凝胶组成,包封的hMSC保留了从76%至87%的良好生存力。我们展示了具有调制强度的不同共混水凝胶复合材料的设计(S参数)和水凝胶网络的松散度(N参数)以控制hMSC从热响应性水凝胶胶囊的释放。我们进一步证明了在3D基质支架内控制释放封装的hMSC的可行性。我们首次通过系统分析报告说,与水凝胶复合材料降解相关的热流变性质与细胞释放动力学之间存在直接相关性。因此,这项工作为干细胞治疗智能载体系统的进一步开发提供了新的见识。
更新日期:2017-10-24
down
wechat
bug