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Construction and Characterization of Broad-Spectrum Promoters for Synthetic Biology
ACS Synthetic Biology ( IF 4.7 ) Pub Date : 2017-11-01 00:00:00 , DOI: 10.1021/acssynbio.7b00258
Sen Yang 1 , Qingtao Liu 1 , Yunfeng Zhang 1 , Guocheng Du 1, 2 , Jian Chen 1, 2 , Zhen Kang 1, 2
Affiliation  

Characterization of genetic circuits and biosynthetic pathways in different hosts always requires promoter substitution and redesigning. Here, a strong, broad-spectrum promoter, Pbs, for Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae was constructed, and it was incorporated into the minimal E. coliB. subtilisS. cerevisiae shuttle plasmid pEBS (5.8 kb). By applying a random mutation strategy, three broad-spectrum promoters Pbs1, Pbs2, and Pbs3, with different strengths were generated and characterized. These broad-spectrum promoters will expand the synthetic biology toolbox for E. coli, B. subtilis, and S. cerevisiae.

中文翻译:

合成生物学广谱启动子的构建与表征

表征不同宿主中的遗传回路和生物合成途径始终需要启动子替代和重新设计。在这里,构建了一个针对大肠杆菌枯草芽孢杆菌酿酒酵母的强大的广谱启动子P bs,并将其整合到最小的大肠杆菌枯草芽孢杆菌酿酒酵母穿梭质粒pEBS(5.8 kb )。通过应用随机突变策略,三个广谱启动子P bs1,P bs2和P bs3生成并表征了不同的强度。这些广谱启动子将扩展大肠杆菌枯草芽孢杆菌酿酒酵母的合成生物学工具箱。
更新日期:2017-11-01
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