Benzotriazole (BTR) and benzothiazole (BTH) derivatives are extensively applied in industrial processes and consumer products, and are thus frequently detected in the environmental matrices. Due to their potential estrogenic effects reported in animal studies, the assessment of human exposure to BTRs and BTHs is important. In this study, a method was developed and validated to determine six BTRs and five BTHs in human urine by using ultra-high performance liquid chromatography coupled with a triple quadrupole mass spectrometry (UHPLC-TQMS) in positive electrospray ionization mode. After de-conjugation by β-glucuronidase, urine samples were liquid-liquid extracted for the measurement of total concentrations of BTRs and BTHs. The linearity, detection limit, precision, recovery, accuracy and matrix effect were evaluated. The limits of detection were less than 0.5 ng/mL. The validated method demonstrated good precision (RSD% < 15%), linearity (R² > 0.99), recovery (>80%) and accuracy (80–100%). The method was successfully applied for the analysis of 22 human urine samples. Four out of eleven targeted compounds were detected in more than half of participants at ng/mL levels.

苯并三唑（BTR）和苯并噻唑（BTH）衍生物广泛应用于工业过程和消费产品中，因此经常在环境基质中检测到。由于在动物研究中报告了其潜在的雌激素作用，因此评估人体接触BTR和BTH的重要性很重要。在这项研究中，开发了一种方法，并通过在正电喷雾电离模式下使用超高效液相色谱结合三重四极杆质谱（UHPLC-TQMS）来测定人尿中的六个BTR和五个BTH。用β解偶联后-葡萄糖醛酸苷酶，尿液样品进行液-液萃取以测定BTR和BTH的总浓度。评价了线性，检测限，精密度，回收率，准确度和基质效应。检测限小于0.5 ng / mL。经过验证的方法显示出良好的精度（RSD％<15％），线性（R ²  > 0.99），回收率（> 80％）和准确性（80–100％）。该方法已成功用于22种人类尿液样品的分析。在超过一半的参与者中，以ng / mL的浓度检测到11种目标化合物中的4种。