当前位置: X-MOL 学术Chem. Res. Toxicol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Influence of DNA Lesions on Polymerase-Mediated DNA Replication at Single-Molecule Resolution
Chemical Research in Toxicology ( IF 4.1 ) Pub Date : 2017-10-23 00:00:00 , DOI: 10.1021/acs.chemrestox.7b00224
Hailey L. Gahlon 1, 2 , Louis J. Romano 3 , David Rueda 1, 2
Affiliation  

Faithful replication of DNA is a critical aspect in maintaining genome integrity. DNA polymerases are responsible for replicating DNA, and high-fidelity polymerases do this rapidly and at low error rates. Upon exposure to exogenous or endogenous substances, DNA can become damaged and this can alter the speed and fidelity of a DNA polymerase. In this instance, DNA polymerases are confronted with an obstacle that can result in genomic instability during replication, for example, by nucleotide misinsertion or replication fork collapse. It is important to know how DNA polymerases respond to damaged DNA substrates to understand the mechanism of mutagenesis and chemical carcinogenesis. Single-molecule techniques have helped to improve our current understanding of DNA polymerase-mediated DNA replication, as they enable the dissection of mechanistic details that can otherwise be lost in ensemble-averaged experiments. These techniques have also been used to gain a deeper understanding of how single DNA polymerases behave at the site of the damage in a DNA substrate. In this review, we evaluate single-molecule studies that have examined the interaction between DNA polymerases and damaged sites on a DNA template.

中文翻译:

DNA损伤对单分子分辨率下聚合酶介导的DNA复制的影响。

忠实地复制DNA是维持基因组完整性的关键方面。DNA聚合酶负责复制DNA,高保真聚合酶可以快速且低错误率进行复制。暴露于外源或内源性物质后,DNA可能会受损,这可能会改变DNA聚合酶的速度和保真度。在这种情况下,DNA聚合酶面临着一个障碍,该障碍可能导致复制过程中的基因组不稳定,例如,核苷酸错误插入或复制叉塌陷。了解DNA聚合酶如何响应受损的DNA底物以了解诱变和化学致癌机制非常重要。单分子技术有助于增进我们对DNA聚合酶介导的DNA复制的了解,因为它们可以剖析机械细节,而这些细节在整体平均实验中可能会丢失。这些技术也已用于更深入地了解单个DNA聚合酶在DNA底物损伤部位的行为。在这篇综述中,我们评估了单分子研究,该研究检查了DNA聚合酶与DNA模板上受损位点之间的相互作用。
更新日期:2017-10-23
down
wechat
bug