Currently, modified Fe₃O₄ magnetic nanoparticles frequently are used as nanocarriers for proteins and enzymes purification. In the present study, Fe₃O₄ magnetic nanoparticles were prepared, and their surfaces were modified with p-aminophenol affinity ligand immobilized by different linkers. The modified nanocarriers were used for the purification of α-amylase from the bovine milk (after precipitation the casein) by affinity purification. To evaluate the effectiveness of the p-aminophenol modified magnetic nanocarriers, the three different types of nanocarriers with different linkers having varying lengths were prepared. All nanocarriers were characterized and validated using FT-IR, SEM, EDX, VSM and XRD analysis methods According to our results, p-aminophenol ligand attached to the nanocarrier by long linkers better separates the α-amylase from the casein free skim milk with 49.66% recovery and 48.18-fold purification efficiency. The results of this study showed that our novel magnetic nanocarriers have the capacity to be used for fast, reproducible and cost-effective purification of α-amylase.

当前，改性的Fe ₃ O ₄磁性纳米颗粒经常被用作蛋白质和酶纯化的纳米载体。在本研究中，制备了Fe ₃ O ₄磁性纳米粒子，并用通过不同接头固定的对氨基苯酚亲和配体修饰了它们的表面。修饰的纳米载体用于通过亲和纯化从牛乳中纯化α-淀粉酶（酪蛋白沉淀后）。评估p的有效性-氨基苯酚改性的磁性纳米载体，制备了具有不同长度的不同接头的三种不同类型的纳米载体。使用FT-IR，SEM，EDX，VSM和XRD分析方法对所有纳米载体进行了表征和验证。根据我们的结果，通过长连接子附着于纳米载体上的对氨基苯酚配体可将无酪蛋白脱脂牛奶中的α-淀粉酶分离得更好（49.66） ％的回收率和48.18倍的纯化效率。这项研究的结果表明，我们新型的磁性纳米载体具有用于快速，可重复生产和具有成本效益的α-淀粉酶纯化的能力。