We designed, constructed, and validated a phage-displayed single-framework synthetic antigen-binding fragment (Fab) library based on a modified trastuzumab framework. High affinity and specificity for human epidermal growth factor receptor-3 (HER3) was shown by an anti-HER3 Fab isolated from the library. It selectively accumulated in a murine HER3-postive xenograft, thus providing a lead for the development of HER3 imaging probes.

中文翻译：

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包含规范和可变互补决定区的组合的单框架合成抗体库。

我们设计，构建和验证了基于修饰的曲妥珠单抗框架的噬菌体展示的单框架合成抗原结合片段（Fab）库。从文库中分离出的抗HER3 Fab显示出对人表皮生长因子受体3（HER3）的高亲和力和特异性。它选择性地在鼠类HER3阳性异种移植物中积累，从而为HER3成像探针的开发提供了线索。