Meat Science ( IF 7.1 ) Pub Date : 2017-10-19 , DOI: 10.1016/j.meatsci.2017.10.012 Qingwei Meng , Shishuai Sun , Yuecheng Sun , Jianan Li , Di Wu , Anshan Shan , Baoming Shi , Baojing Cheng
A 2 × 2 factorial experiment was conducted to investigate the effects of dietary lecithin and l-carnitine on fatty acid composition and lipid-metabolic genes expression in subcutaneous fat and longissimus thoracis of growing-finishing pigs. 160 barrows were assigned to 4 treatments consisting of 8 replicates with 5 pigs in each. The total PUFA, C18:2n-6 and C18:3n-3 in subcutaneous fat were increased by lecithin but the effect of lecithin was dependent of l-carnitine where supplementation of lecithin together with l-carnitine decreased total PUFA, C18:2n-6 and C18:3n-3. l-Carnitine increased the intramuscular fat content when supplemented with lecithin but no effect was observed without lecithin supplementation. l-Carnitine increased the mRNA expression of CPT1A, HSL, FABP4 and CRAT; and reduced the mRNA expression of FAS and ACCα in subcutaneous fat. Lecithin increased the mRNA expression of ACCα and ME1 in longissimus thoracis. l-Carnitine increased the mRNA expression of FAS in longissimus thoracis when supplemented with lecithin but no effect was observed without lecithin supplementation.
中文翻译:
在皮下脂肪和脂肪酸组成和脂质代谢的基因的表达的膳食卵磷脂和L-肉碱的影响最长胸外科生长肥育猪的
A 2×2阶乘实验以调查膳食卵磷脂和的影响升在皮下脂肪肉碱脂肪酸组成和脂质代谢的基因的表达和最长胸外科生长肥育猪的。将160头公猪分配到4种处理方法中,每组5头猪,共进行8次重复。卵磷脂可增加皮下脂肪中的总PUFA,C18:2n-6和C18:3n-3,但卵磷脂的作用取决于l-肉碱,其中补充卵磷脂和l-肉碱可降低总PUFA,C18:2n- 6和C18:3n-3。升-肉碱在添加卵磷脂时会增加肌内脂肪含量,但未添加卵磷脂则未见效果。l-肉碱增加CPT1A,HSL,FABP4和CRAT的mRNA表达; 降低皮下脂肪中FAS和ACCα的mRNA表达。卵磷脂增加的mRNA表达ACCα和ME1在最长胸外科。升肉碱增加的mRNA表达FAS在最长胸外科当与卵磷脂补充但不补充卵磷脂没有观察到效果。