A new screen-printed carbon electrode (SPCE) with multiple working electrodes and one signal output channel without counter and reference electrodes was designed. The multiple working electrodes can be individually modified for each target of interest. The SPCE contained one signal output channel, making the immuniosensor be realized by common single-channel electrochemical workstation. The counter and reference electrodes were independent of disposable SPCE, reducing costs and eliminating precious metal pollution. Platinum network as counter electrode improved the reproducibility of the SPCE. Moreover, method of generating hydrogel on working electrode was used to enhance the conductivity of SPCE. Based on this, a multiplexed single channel label-free amperometric immuniosensor for four tumor markers, namely, squamous cell carcinoma antigen (SCCA), fragment antigen 21-1 (Cyfra21-1), carbohydrate antigen 125 (CA125), and neuron specific enolase (NSE) was developed, and the corresponding detection limits were 5.5 pg mL⁻¹, 4.8 pg mL⁻¹, 0.0054 U mL⁻¹ and 2.3 pg mL⁻¹, respectively. The sensitivity of this immunosensor was 0.83 µA (lg(ng mL⁻¹))⁻¹ for SCCA, 1.92 µA (lg(ng mL⁻¹))⁻¹ for Cyfra21-1, 4.75 µA (lg(U mL⁻¹))⁻¹ for CA125 and 2.40 µA (lg(ng mL⁻¹))⁻¹ for NSE. Among them, the sensitivities of CA125 and NSE were four-fold higher than those of the previous works.

设计了一种新型的丝网印刷碳电极（SPCE），该电极具有多个工作电极和一个不带对电极和参比电极的信号输出通道。多个工作电极可以针对每个感兴趣的目标进行单独修改。SPCE包含一个信号输出通道，使免疫传感器可以通过普通的单通道电化学工作站来实现。对电极和参比电极独立于一次性SPCE，可降低成本并消除贵金属污染。铂网络作为对电极提高了SPCE的重现性。此外，通过在工作电极上生成水凝胶的方法来提高SPCE的电导率。基于此，针对四个肿瘤标记物（即，^-1，4.8皮克毫升^-1，0.0054ù毫升^-1和2.3皮克毫升^-1分别。该免疫传感器的灵敏度对于SCCA为0.83 µA（lg（ng mL ^-1））- ¹，对于Cyfra21-1为1.92 µA（lg（ng mL ^-1））- ^1，为4.75 µA（lg（U mL ^-1） ）^-1为CA125和2.40μA（LG（每mL ^-1）） ^-1为NSE。其中，CA125和NSE的敏感性比以前的研究提高了四倍。