Herpes simplex virus 1 (HSV-1) is a widespread pathogen that persists for life, replicating in surface tissues and establishing latency in peripheral ganglia. Increasingly, molecular studies of latency use cultured neuron models developed using recombinant viruses such as HSV-1 GFP-US11, a derivative of strain Patton expressing green fluorescent protein (GFP) fused to the viral US11 protein. Visible fluorescence follows viral DNA replication, providing a real time indicator of productive infection and reactivation. Patton was isolated in Houston, Texas, prior to 1973, and distributed to many laboratories. Although used extensively, the genomic structure and phylogenetic relationship to other strains is poorly known. We report that wild type Patton and the GFP-US11 recombinant contain the full complement of HSV-1 genes and differ within the unique regions at only eight nucleotides, changing only two amino acids. Although isolated in North America, Patton is most closely related to Asian viruses, including KOS63.

单纯疱疹病毒 1 (HSV-1) 是一种广泛存在的病原体，可终生存在，在表面组织中复制并在外周神经节中建立潜伏期。潜伏期的分子研究越来越多地使用使用重组病毒开发的培养神经元模型，例如 HSV-1 GFP-US11，它是表达与病毒 US11 蛋白融合的绿色荧光蛋白 (GFP) 的 Patton 菌株的衍生物。病毒 DNA 复制后会出现可见荧光，提供有效感染和重新激活的实时指示。1973 年之前，巴顿在德克萨斯州休斯顿被隔离，并被分发到许多实验室。尽管广泛使用，但其基因组结构和与其他菌株的系统发育关系却鲜为人知。我们报道野生型 Patton 和 GFP-US11 重组体含有 HSV-1 基因的完整互补体，并且在独特区域内仅存在 8 个核苷酸的差异，仅改变了两个氨基酸。尽管在北美被隔离，但 Patton 与包括 KOS63 在内的亚洲病毒关系最为密切。