Over the past decade, there has been a great deal of interest in the integration of nanotechnology and carbohydrates. The advances in glyconanotechnology have allowed the creation of different bioactive glyconanostructures for different types of medical applications, especially for drug delivery and release systems. Therefore, the use of more efficient biocompatible nanocarriers with high loading capacity, low overall toxicity and receptor-mediated endocytosis specificity is still in focus for the enhancement of the therapeutic effect. Conjugation of sugar derivatives onto gold nanoparticles presents unique properties that include a wide array of assembling models and size-related electronic, magnetic and optical properties. Here, pH-responsive drug-conjugated glycopolymer-coated gold nanoparticles were prepared by functionalization of gold nanoparticles with thiol-terminated glycopolymers and then subsequent conjugation of doxorubicin (DOX). Among the four different glycopolymers, their drug release, physicochemical characterization (spectroscopy, particle size and surface charge) and in vitro bioapplications with four different cell lines were compared. As a result, pH-sensitive drug delivery via sugar-coated AuNPs was performed thanks to hydrazone linkages between glycopolymers and DOX. Comparative viability tests also demonstrated the efficiency of glycopolymer–DOX conjugates by fluorescence cell imaging. The obtained results reveal that AuNP homoglycopolymer DOX conjugates (P4D) have significant potential, especially in human neuroblastoma cells in comparison to cervical cancer cells and lung cancer cells.

中文翻译：

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pH响应型糖聚合物纳米颗粒可用于靶向抗癌药物†

在过去的十年中，人们对纳米技术和碳水化合物的整合产生了浓厚的兴趣。糖纳米技术的进步已允许针对不同类型的医学应用，特别是对于药物递送和释放系统，创建不同的生物活性糖纳米结构。因此，使用具有高负载能力，低总体毒性和受体介导的内吞作用特异性的更有效的生物相容性纳米载体仍然是提高治疗效果的焦点。糖衍生物在金纳米颗粒上的缀合具有独特的性能，包括各种组装模型以及与尺寸相关的电子，磁性和光学性能。这里，pH响应药物共轭糖聚合物包被的金纳米颗粒的制备方法是：用巯基封端的糖聚合物对金纳米颗粒进行功能化，然后再进行阿霉素（DOX）的缀合。在四种不同的糖聚合物中，它们的药物释放，理化特性（光谱，粒径和表面电荷）和比较了四种不同细胞系的体外生物应用。结果，由于糖聚合物和DOX之间的键，通过糖衣AuNPs进行了pH敏感的药物递送。比较生存力测试还通过荧光细胞成像证明了糖聚合物-DOX共轭物的效率。获得的结果表明，与子宫颈癌细胞和肺癌细胞相比，AuNP均糖聚合物DOX缀合物（P4D）具有显着的潜力，尤其是在人神经母细胞瘤细胞中。