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Chiral recognition of tryptophan enantiomers using chitosan-capped silver nanoparticles: Scanometry and spectrophotometry approaches
Talanta ( IF 6.1 ) Pub Date : 2017-10-18 , DOI: 10.1016/j.talanta.2017.10.005
Marzieh Jafari , Javad Tashkhourian , Ghodratollah Absalan

A new, fast and inexpensive colorimetric sensor was developed for chiral recognition of tryptophan enantiomers using chitosan-capped silver nanoparticles. The function of the sensor was based on scanometry and spectrophotometry of the colored product of a reaction solution containing a mixture of chitosan-capped silver nanoparticles, phosphate buffer and tryptophan enantiomers. The image of the colored solution was taken using the scanometer and the corresponding color values were obtained using Photoshop software which subsequently were used for optimization of the experimental parameters as the analytical signal. Two types of color values system were investigated: RGB (red, green and blue values) and CMYK (cyan, magenta, yellow and black values). The color values indicated that L-tryptophan had better interaction than D-tryptophan with chitosan-capped silver nanoparticles. A linear relationship between the analytical signal and the concentration of L-tryptophan was obtained in the concentration range of 1.3 × 10−5–4.6 × 10−4 mol L−1. Detection limits, were obtained to be 2.1 × 10−6, 2.4 × 10−6 and 3.8 × 10−6 mol L−1 for L-tryptophan based on R (red), G (green) and B (blue) values, respectively.



中文翻译:

使用壳聚糖封端的银纳米粒子手性识别色氨酸对映体:扫描和分光光度法

开发了一种新型,快速且廉价的比色传感器,用于使用壳聚糖封端的银纳米颗粒进行手性识别色氨酸对映体。传感器的功能基于包含壳聚糖封端的银纳米颗粒,磷酸盐缓冲液和色氨酸对映体混合物的反应溶液的有色产物的扫描测定和分光光度法。使用扫描仪获取有色溶液的图像,并使用Photoshop软件获得相应的色值,随后将其用于优化实验参数作为分析信号。研究了两种类型的颜色值系统:RGB(红色,绿色和蓝色值)和CMYK(青色,品红色,黄色和黑色值)。颜色值表示L色氨酸与D-色氨酸与壳聚糖封端的银纳米粒子的相互作用更好。在1.3×10 -5 –4.6×10 -4  mol L -1的浓度范围内,分析信号与L-色氨酸的浓度之间存在线性关系。检测限,得到为2.1×10 -6 2.4×10 -6和3.8×10 -6 摩尔大号-1大号色氨酸基于R(红),G(绿)和B(蓝)值,分别。

更新日期:2017-10-18
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