Genetically encoded Förster resonance energy transfer (FRET) biosensors have been instrumental to our understanding of how intracellular signalling is organised and regulated within cells. In the last decade, the toolbox, dynamic range and applications of these sensors have expanded beyond basic cell biology applications. In particular, FRET biosensors have shed light onto the mechanisms that control the intracellular organisation of G protein-coupled receptor (GPCR) signalling and have allowed the visualisation of signalling events with unprecedented temporal and spatial resolution. Here we review the use of these sensors in the GPCR field and how it has already provided invaluable advances towards our understanding of the complexity of GPCR signalling.

遗传编码的Förster共振能量转移（FRET）生物传感器已经帮助我们了解细胞内信号在细胞内的组织和调控方式。在过去的十年中，这些传感器的工具箱，动态范围和应用已经扩展到基本的细胞生物学应用之外。特别是，FRET生物传感器已经阐明了控制G蛋白偶联受体（GPCR）信号的细胞内组织的机制，并允许以前所未有的时间和空间分辨率可视化信号事件。在这里，我们回顾了这些传感器在GPCR领域的使用以及它如何为我们理解GPCR信号的复杂性提供了宝贵的进展。