In this work, a highly-efficient competitive method-based electrochemiluminescence (ECL) immunosensor was proposed based on {[Ru(bpy)₃][Cu_2xNi_2(1−x)(ox)₃]}_n (Cu/Ni/Ru) as luminophor to efficiently detect aflatoxins B1 (AFB1). Cu/Ni/Ru exhibited excellent ECL behavior. While polyethyleneimine capped silicon dioxide ([email protected]₂) could decrease the ECL performance of Cu/Ni/Ru due that PEI could destroy the structure of Cu/Ni/Ru via producing the complex between PEI and metal ions (Cu(II)/Ni(II)), inducing the release of Ru(bpy)₃²⁺. Since Au nanoparticles can directly combine with antibody and antigen, the Cu/Ni/Ru and [email protected]₂ was functionalized by Au nanoparticles. The quantitative detection of AFB1 was based on the competitive binding between AFB1-bovine serum albumin labeled [email protected]₂ ([email protected]₂-AFB1-BSA) and free AFB1 with antibody-AFB1 which immobilized on Au-Cu/Ni/Ru. The ECL signal increased with augmenting the concentrations of the free AFB1 due to less [email protected]₂-AFB1-BSA combining with antibodies. Under optimal conditions, the proposed immunosensor exhibited a wide linear range from 0.01 ng mL⁻¹ to 100 ng mL⁻¹ with a detection limit of 0.0039 ng mL⁻¹ (S/N = 3). The proposed immunosensor also provides a promising approach for ultrasensitive detection of other mycotoxins.

在这项工作中，基于{[Ru（bpy）₃ ] [Cu _2x Ni _2（1-x）（ox）₃ ]} _n（Cu / Ni / Ru）作为发光体可有效检测黄曲霉毒素B1（AFB1）。Cu / Ni / Ru表现出优异的ECL行为。聚乙烯亚胺封盖的二氧化硅（[受电子邮件保护] ₂）可能会降低Cu / Ni / Ru的ECL性能，这是因为PEI可能通过在PEI和金属离子之间形成络合物而破坏Cu / Ni / Ru的结构（Cu（II） / Ni（II）），诱导释放Ru（bpy）₃ ²⁺。由于金纳米颗粒可以直接与抗体和抗原结合，因此铜/镍/钌和[电子邮件受到保护]₂通过Au纳米粒子功能化。AFB1的定量检测是基于标记为[电子邮件保护] ₂（[电子邮件保护] ₂ -AFB1-BSA）的AFB1牛血清白蛋白与固定在Au-Cu / Ni /上的抗体-AFB1之间的竞争性结合。茹。ECL信号随着游离AFB1浓度的增加而增加，这是因为较少的[受电子邮件保护的] ₂ -AFB1-BSA与抗体结合。在最佳条件下，拟议的免疫传感器表现出从0.01 ng mL ^-1到100 ng mL ^-1的宽线性范围，检测极限为0.0039 ng mL ^-1（S / N = 3）。提出的免疫传感器还为其他真菌毒素的超灵敏检测提供了一种有前途的方法。