It is important to design a nice electrochemiluminescence (ECL) biological nanomaterial for fabricating sensitive ECL immunosensor to detect tumor markers. Most reported ECL nanomaterial was decorated by a number of mono-luminophore. Here, we report a novel ECL nanomaterial assembled by dual luminophores perylenetetracarboxylic acid (PTCA) and carbon quantum dots (CQDs). In the ECL nanomaterial, graphene was chosen as nanocarrier. Significant ECL intensity increases are seen in the ECL nanomaterial, which was interpreted with the proposed synergistic promotion ECL meachanism of PTCA and CQDs. Furthermore, this ECL nanomaterial was used to label secondary antibody and fabricate a sandwiched carcinoembryonic antigen (CEA) immunosensor. The CEA immunosensor exhibits high sensitivity and the linear semilogarithmical range was from 0.001 fg mL⁻¹ to 1 ng mL⁻¹ with low detection limit 0.00026 fg mL⁻¹. And the CEA immunosensor is also suitable for various cancers’ sample detection providing potential specific applications in diagnostics.

设计一种良好的电化学发光（ECL）生物纳米材料对于制造灵敏的ECL免疫传感器以检测肿瘤标志物非常重要。大多数报道的ECL纳米材料都由许多单荧光体修饰。在这里，我们报告由双发光luphor四羧酸（PTCA）和碳量子点（CQDs）组装的新型ECL纳米材料。在ECL纳米材料中，石墨烯被选作纳米载体。在ECL纳米材料中可以看到ECL强度显着增加，这可以通过提出的PTCA和CQD协同增效ECL机制来解释。此外，该ECL纳米材料用于标记第二抗体并制造夹心的癌胚抗原（CEA）免疫传感器。CEA免疫传感器显示出高灵敏度，线性半对数范围为0.001 fg mL^-1至1 ng mL ^-1，低检测限0.00026 fg mL ^-1。CEA免疫传感器也适用于各种癌症的样本检测，从而在诊断中提供了潜在的特定应用。