New M2 blockers effective against the ubiquitous amantadine-resistant S31N M2 mutation in influenza A are needed. Six copper complexes, 2, 4, 6, 8, 9, and 10, were synthesized and found to block both wild type and S31N M2. Free Cu²⁺ also blocks M2 S31N but not S31N/H37A. The copper complexes do not block M2 H37A (either S31 or S31N). The complexes were effective against three influenza A strains in cell-culture assays, but less toxic to cells than CuCl₂. For example 4, Cu(cyclooctylamineiminodiacetate), which was stable at pH > 4 in the buffers used, had an EC₅₀ against A/Calif/07/2009 H1N1 of 0.7 ± 0.1 μM with a CC₅₀ of 147 μM (therapeutic index, averaged over three strains, 67.8). In contrast, CuCl₂ had an EC₅₀ of 3.8 ± 0.9 μM and CC₅₀ of 19 μM. Because M2 H37 is highly conserved, these complexes show promise for further testing as drugs against all strains of influenza A.

需要新型的M2阻滞剂，以有效抵抗甲型流感中普遍存在的耐金刚烷胺的S31N M2突变。六铜络合物，2，4，6，8，9，和10，被合成并发现阻断野生型和S31N M2。游离Cu ²⁺也会阻止M2 S31N，但不会阻止S31N / H37A。铜络合物不会阻止M2 H37A（S31或S31N）。该复合物在细胞培养试验中可有效对抗三种甲型流感病毒，但对细胞的毒性不如CuCl ₂。例如，在所用的缓冲液中，pH（> 4）稳定的Cu（环辛基胺亚氨基二乙酸酯）的EC ₅₀相对于A / Calif / 07/2009 H1N1为0.7±0.1μM，CC ₅₀为147μM（治疗指数，三个菌株的平均值为67.8）。相反，CuCl ₂的EC ₅₀为3.8±0.9μM，CC ₅₀为19μM。由于M2 H37是高度保守的，这些配合物显示出有望作为针对所有A型流感病毒的药物进行进一步测试的希望。