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Amperometric Detection of dsDNA using an Acridine-Orange-Modified Glucose Oxidase.
ChemPlusChem ( IF 3.4 ) Pub Date : 2017-10-10 , DOI: 10.1002/cplu.201700373
Daliborka Jambrec 1 , Klaus Lammers 1 , Tim Bobrowski 1 , Sascha Pöller 1 , Wolfgang Schuhmann 1 , Adrian Ruff 1
Affiliation  

Invited for this month's cover is the group of Prof. Dr. Wolfgang Schuhmann, Dr. Daliborka Jambrec and Dr. Adrian Ruff at Ruhr-Universität in Bochum, Germany. The cover picture shows a novel procedure for the preferential post-hybridization labeling of double-stranded DNA based on the intercalating compound acridine orange, which was covalently bound to glucose oxidase. Labeling with a highly active biocatalyst allows for a simple and sequence-independent amplification of the signal proportional to the amount of hybridized DNA that may be coupled with other amplification strategies. Read the full text of the article at 10.1002/cplu.201700279.

中文翻译:

使用an啶橙修饰的葡萄糖氧化酶进行dsDNA的安培检测。

本月的封面邀请了德国波鸿鲁尔大学的Wolfgang Schuhmann博士,Daliborka Jambrec博士和Adrian Ruff博士一组。封面图片显示了基于嵌入化合物compound啶橙的双链DNA优先杂交后标记的新方法,该插入化合物与葡萄糖氧化酶共价结合。用高度活性的生物催化剂进行标记可以对信号进行简单且不依赖序列的扩增,该信号与可与其他扩增策略偶联的杂交DNA量成正比。阅读文章全文10.1002 / cplu.201700279。
更新日期:2017-10-10
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