We report herein a novel chemical-genetic method for assaying RNA localization within living cells. RNA localization is critical for normal physiology as well as the onset of cancer and neurodegenerative disorders. Despite its importance, there is a real lack of chemical methods to directly assay RNA localization with high resolution in living cells. Our novel approach relies on in situ nucleobase oxidation by singlet oxygen generated from spatially confined fluorophores. We demonstrate that our novel method can identify RNA molecules localized within specific cellular compartments. We anticipate that this platform will provide the community with a much-needed methodology for tracking RNA localization within living cells, and set the stage for systematic large scale analysis of RNA localization in living systems.

中文翻译：

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用空间受限的核酸碱基氧化原位测定RNA定位

我们在这里报告了一种新型的化学遗传方法，用于分析活细胞内的RNA定位。RNA定位对于正常生理以及癌症和神经退行性疾病的发作至关重要。尽管它很重要，但实际上仍然缺乏化学方法来直接测定活细胞中高分辨率的RNA定位。我们的新颖方法依赖于原位从空间受限的荧光团产生的单线态氧使核碱基氧化。我们证明了我们的新方法可以识别位于特定细胞室内的RNA分子。我们预计，该平台将为社区提供急需的方法来跟踪活细胞内的RNA定位，并为系统地大规模分析活系统中的RNA定位奠定基础。