In this study, freeze dried methanogenic biomass (FDMB) was used as inoculum in place of conventional, non-dried biomass for biochemical methane potential (BMP) assays and as a bioaugment to improve upset digester recovery. Methanogenic biomass was freeze dried and stored in an air atmosphere. Significant methanogenic activity was preserved in FDMB even with drying and storage in air; specific methanogenic activity (SMA) values were 65 ± 4.5% and 42 ± 10.4% for hydrogen:carbon dioxide (H₂:CO₂) and acetate, respectively, compared to non-dried biomass. There was no significant difference in BMP results for the four substrates tested (glucose, non-fat dry milk, thin stillage and dog food) when using FDMB and non-dried biomass as inocula. However, BMP assays using FDMB inocula took longer to complete. Methane (CH₄) production from digesters exposed to a model toxicant (oxygen [O₂]) recovered faster when bioaugmented with FDMB compared to digesters that received autoclaved biomass or no bioaugmentation. Methanogen communities in all digesters before toxicant exposure and bioaugmentation were similar. However, bioaugmented and non-augmented digester communities were significantly different during the recovery phase after toxicant exposure. Sequences similar to Methanospirillum were related to improved performance of the FDMB bioaugmented digesters. FDMB could be developed as a standard inoculum for BMP analyses and to bioaugment anaerobic digesters for improved performance. These results may encourage developing customized, dried methanogenic biomass for specific anaerobic biotechnology applications.

中文翻译：

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冻干生物质的生化甲烷潜力测定和厌氧消化池生物强化

在这项研究中，冷冻干燥的产甲烷生物质（FDMB）代替常规的非干燥生物质用作生物化学甲烷潜力（BMP）测定的接种物，并用作提高厌氧消化器回收率的生物强化剂。将产甲烷的生物质冷冻干燥并储存在空气中。即使在空气中干燥和储存，FDMB中仍保留了显着的产甲烷活性。氢气：二氧化碳（H ₂：CO ₂与非干燥生物质相比）。当使用FDMB和非干燥生物质作为接种物时，测试的四种底物（葡萄糖，脱脂奶粉，稀釜馏物和狗粮）的BMP结果没有显着差异。但是，使用FDMB接种液进行BMP分析需要更长的时间才能完成。与使用高压灭菌的生物质或未进行生物强化处理的消化池相比，使用FDMB进行生物强化处理时，暴露于标准毒物（氧气[O ₂ ]）的消化池中甲烷（CH ₄）的回收速度更快。在接触毒物和生物强化之前，所有消化池中的产甲烷菌群落相似。但是，在接触毒物后的恢复阶段，生物强化和非强化消化池群落差异显着。类似于的序列Methanospirillum均与改进的FDMB bioaugmented沼气池的性能。FDMB可以作为BMP分析的标准接种物，也可以作为生物强化厌氧消化器的标准接种物，以提高性能。这些结果可能会鼓励开发定制的，干燥的产甲烷生物质，以用于特定的厌氧生物技术应用。