A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell super-resolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP tag, together with a noncovalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells.

中文翻译：

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带有内源性SNAP标记融合蛋白的活细胞的双色810 nm STED纳米显微镜

已经开发了810 nm STED纳米显微镜设置和两种荧光染料（Si-若丹明680SiR和碳卡吡咯啉610CP）的适当组合，用于近红外活细胞超分辨率成像。使用SNAP标签的CRISPR / Cas9方法内源性标记的波形蛋白与非共价微管蛋白标签一起，为活细胞中的细胞骨架提供了可靠且细胞间可重现的双色共聚焦和STED成像。