The present study is focused on investigating the immunoprotective effects of CpG-ODN/Poly(I:C) combined with the viral glycoprotein gp90 protein against reticuloendotheliosis virus (REV) infection in chickens. REV's gp90 gene was amplified from the REV-infected cells and expressed in Escherichia coli (E.coli). The expressed products, upon purification, were inoculated into 7-day-old chickens with PBS, CpG-ODN or Poly(I:C) adjuvant; Two booster inoculations were then conducted, and then each chicken was challenged. The presence of REV-antibodies in serum was determined weekly after the first vaccination. The viremia and immunosuppressive effects of REV infection were also monitored after the challenge. The neutralizing effects of the antisera were tested in vitro. The results showed that the recombinant gene containing REV gp90 gene was expressed into the recombinant protein with a size of 51 Kilo Dalton (KD), which could be recognized by a monoclonal antibody (MAb) against the gp90 protein. The viremia and immunosuppressive effects of avian influenza virus (AIV) vaccine caused by REV challenge in CpG-ODN group and in Poly(I:C) group were dramatically decreased. REV antibody with low titers was induced in gp90 group and the inoculated chickens were partly protected. Compared with those in gp90 group, the titers and the positive ratios of REV antibody in CpG+gp90 group were significantly increased, whereas the viremia and immunosuppressive effects of AIV vaccine caused by REV infection were significantly decreased. In the Poly(I:C) +gp90 group, the viremia and immunosuppressive effects caused by REV infection were also dramatically decreased, although REV antibody responses were softly increased. The diluted antisera from the vaccinated chickens in both groups could completely inhibit the replication of REV in chick fibroblast cells (CEF). Hence, it can be concluded that CpG-ODN or the Poly(I:C) adjuvant can enhance the antiviral effects of the REV subunit vaccine against REV infection, which may result from different mechanisms.

本研究的重点是调查CpG-ODN / Poly（I：C）结合病毒糖蛋白gp90蛋白对鸡网状内皮病病毒（REV）感染的免疫保护作用。从REV感染的细胞中扩增出REV的gp90基因，并在大肠杆菌（大肠杆菌（E.coli））。纯化后，将表达的产物用PBS，CpG-ODN或Poly（I：C）佐剂接种到7天大的鸡中；然后进行两次加强接种，然后攻击每只鸡。首次接种后每周检查一次血清中REV抗体的存在。攻击后还监测REV感染的病毒血症和免疫抑制作用。在体外测试了抗血清的中和作用。结果表明，含有REV gp90基因的重组基因以51 Kilo Dalton的大小表达到重组蛋白中。（KD），可以被针对gp90蛋白的单克隆抗体（MAb）识别。CpG-ODN组和Poly（I：C）组的REV攻击引起的禽流感病毒（AIV）疫苗的病毒血症和免疫抑制作用大大降低。在gp90组中诱导了低滴度的REV抗体，部分保护了接种的鸡。与gp90组相比，CpG + gp90组REV抗体的滴度和阳性率显着增加，而REV感染引起的AIV疫苗的病毒血症和免疫抑制作用则明显降低。在Poly（I：C）+ gp90组中，尽管REV抗体反应轻度增加，但REV感染引起的病毒血症和免疫抑制作用也显着降低。两组接种疫苗的鸡的稀释抗血清可以完全抑制REV在雏鸡成纤维细胞（CEF）中的复制。因此，可以得出结论，CpG-ODN或Poly（I：C）佐剂可以增强REV亚单位疫苗针对REV感染的抗病毒作用，这可能是由于不同的机制引起的。