Reproducibility has been one of the biggest hurdles faced when attempting to develop quantitative protocols for MALDI mass spectrometry. The heterogeneous nature of sample recrystallization has made automated sample acquisition somewhat “hit and miss” with manual intervention needed to ensure that all sample spots have been analyzed. In this review, we explore the last 30 years of literature and anecdotal evidence that has attempted to address and improve reproducibility in MALDI MS. Though many methods have been attempted, we have discovered a significant publication history surrounding the use of nitrocellulose as a substrate to improve homogeneity of crystal formation and therefore reproducibility. We therefore propose that this is the most promising avenue of research for developing a comprehensive and universal preparation protocol for quantitative MALDI MS analysis. © 2016 Wiley Periodicals, Inc. Mass Spec Rev 37:217–228, 2018.

中文翻译：

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寻求提高MALDI质谱的重现性

尝试开发MALDI质谱定量方案时，可重复性一直是面临的最大障碍之一。样品重结晶的异质性使得自动样品采集有些“碰巧”，需要人工干预以确保所有样品点均已被分析。在这篇综述中，我们探索了过去30年的文献和轶事证据，这些文献和证据试图解决和提高MALDI MS的重现性。尽管已经尝试了许多方法，但是我们发现了围绕硝化纤维素作为底物来改善晶体形成的均质性并因此提高了可再现性的重要出版历史。因此，我们建议这是开发用于定量MALDI MS分析的全面通用的制备方案的最有前途的研究途径。©2016 Wiley Periodicals，Inc.质谱修订版37：217–228，2018年。