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An integrated expression atlas of miRNAs and their promoters in human and mouse
Nature Biotechnology ( IF 46.9 ) Pub Date : 2017-08-21 00:00:00 , DOI: 10.1038/nbt.3947 Derek de Rie , , Imad Abugessaisa , Tanvir Alam , Erik Arner , Peter Arner , Haitham Ashoor , Gaby Åström , Magda Babina , Nicolas Bertin , A Maxwell Burroughs , Ailsa J Carlisle , Carsten O Daub , Michael Detmar , Ruslan Deviatiiarov , Alexandre Fort , Claudia Gebhard , Daniel Goldowitz , Sven Guhl , Thomas J Ha , Jayson Harshbarger , Akira Hasegawa , Kosuke Hashimoto , Meenhard Herlyn , Peter Heutink , Kelly J Hitchens , Chung Chau Hon , Edward Huang , Yuri Ishizu , Chieko Kai , Takeya Kasukawa , Peter Klinken , Timo Lassmann , Charles-Henri Lecellier , Weonju Lee , Marina Lizio , Vsevolod Makeev , Anthony Mathelier , Yulia A Medvedeva , Niklas Mejhert , Christopher J Mungall , Shohei Noma , Mitsuhiro Ohshima , Mariko Okada-Hatakeyama , Helena Persson , Patrizia Rizzu , Filip Roudnicky , Pål Sætrom , Hiroki Sato , Jessica Severin , Jay W Shin , Rolf K Swoboda , Hiroshi Tarui , Hiroo Toyoda , Kristoffer Vitting-Seerup , Louise Winteringham , Yoko Yamaguchi , Kayoko Yasuzawa , Misako Yoneda , Noriko Yumoto , Susan Zabierowski , Peter G Zhang , Christine A Wells , Kim M Summers , Hideya Kawaji , Albin Sandelin , Michael Rehli , Yoshihide Hayashizaki , Piero Carninci , Alistair R R Forrest , Michiel J L de Hoon
Nature Biotechnology ( IF 46.9 ) Pub Date : 2017-08-21 00:00:00 , DOI: 10.1038/nbt.3947 Derek de Rie , , Imad Abugessaisa , Tanvir Alam , Erik Arner , Peter Arner , Haitham Ashoor , Gaby Åström , Magda Babina , Nicolas Bertin , A Maxwell Burroughs , Ailsa J Carlisle , Carsten O Daub , Michael Detmar , Ruslan Deviatiiarov , Alexandre Fort , Claudia Gebhard , Daniel Goldowitz , Sven Guhl , Thomas J Ha , Jayson Harshbarger , Akira Hasegawa , Kosuke Hashimoto , Meenhard Herlyn , Peter Heutink , Kelly J Hitchens , Chung Chau Hon , Edward Huang , Yuri Ishizu , Chieko Kai , Takeya Kasukawa , Peter Klinken , Timo Lassmann , Charles-Henri Lecellier , Weonju Lee , Marina Lizio , Vsevolod Makeev , Anthony Mathelier , Yulia A Medvedeva , Niklas Mejhert , Christopher J Mungall , Shohei Noma , Mitsuhiro Ohshima , Mariko Okada-Hatakeyama , Helena Persson , Patrizia Rizzu , Filip Roudnicky , Pål Sætrom , Hiroki Sato , Jessica Severin , Jay W Shin , Rolf K Swoboda , Hiroshi Tarui , Hiroo Toyoda , Kristoffer Vitting-Seerup , Louise Winteringham , Yoko Yamaguchi , Kayoko Yasuzawa , Misako Yoneda , Noriko Yumoto , Susan Zabierowski , Peter G Zhang , Christine A Wells , Kim M Summers , Hideya Kawaji , Albin Sandelin , Michael Rehli , Yoshihide Hayashizaki , Piero Carninci , Alistair R R Forrest , Michiel J L de Hoon
MicroRNAs (miRNAs) are short non-coding RNAs with key roles in cellular regulation. As part of the fifth edition of the Functional Annotation of Mammalian Genome (FANTOM5) project, we created an integrated expression atlas of miRNAs and their promoters by deep-sequencing 492 short RNA (sRNA) libraries, with matching Cap Analysis Gene Expression (CAGE) data, from 396 human and 47 mouse RNA samples. Promoters were identified for 1,357 human and 804 mouse miRNAs and showed strong sequence conservation between species. We also found that primary and mature miRNA expression levels were correlated, allowing us to use the primary miRNA measurements as a proxy for mature miRNA levels in a total of 1,829 human and 1,029 mouse CAGE libraries. We thus provide a broad atlas of miRNA expression and promoters in primary mammalian cells, establishing a foundation for detailed analysis of miRNA expression patterns and transcriptional control regions.
中文翻译：
miRNA及其启动子在人和小鼠中的整合表达图谱
微小RNA（miRNA）是短的非编码RNA，在细胞调节中起关键作用。作为第五版哺乳动物基因组功能注释（FANTOM5）项目的一部分，我们通过对492个短RNA（sRNA）文库进行深度测序，并结合了Cap分析基因表达（CAGE），创建了一个完整的miRNA及其启动子表达图谱。数据来自396个人类和47个小鼠RNA样本。鉴定出1,357个人类miRNA和804个小鼠miRNA的启动子，并显示出物种之间的强序列保守性。我们还发现，初级和成熟miRNA的表达水平是相关的，这使我们能够使用初级miRNA的测量值作为总共1,829个人和1,029小鼠CAGE文库中成熟miRNA的水平的代表。因此，我们提供了哺乳动物原始细胞中miRNA表达和启动子的广泛地图集，
更新日期：2017-09-12
中文翻译：
miRNA及其启动子在人和小鼠中的整合表达图谱
微小RNA（miRNA）是短的非编码RNA，在细胞调节中起关键作用。作为第五版哺乳动物基因组功能注释（FANTOM5）项目的一部分，我们通过对492个短RNA（sRNA）文库进行深度测序，并结合了Cap分析基因表达（CAGE），创建了一个完整的miRNA及其启动子表达图谱。数据来自396个人类和47个小鼠RNA样本。鉴定出1,357个人类miRNA和804个小鼠miRNA的启动子，并显示出物种之间的强序列保守性。我们还发现，初级和成熟miRNA的表达水平是相关的，这使我们能够使用初级miRNA的测量值作为总共1,829个人和1,029小鼠CAGE文库中成熟miRNA的水平的代表。因此，我们提供了哺乳动物原始细胞中miRNA表达和启动子的广泛地图集，
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