B-cell responses result in clonal expansion, and can occur in a variety of tissues. To define how B-cell clones are distributed in the body, we sequenced 933,427 B-cell clonal lineages and mapped them to eight different anatomic compartments in six human organ donors. We show that large B-cell clones partition into two broad networks—one spans the blood, bone marrow, spleen and lung, while the other is restricted to tissues within the gastrointestinal (GI) tract (jejunum, ileum and colon). Notably, GI tract clones display extensive sharing of sequence variants among different portions of the tract and have higher frequencies of somatic hypermutation, suggesting extensive and serial rounds of clonal expansion and selection. Our findings provide an anatomic atlas of B-cell clonal lineages, their properties and tissue connections. This resource serves as a foundation for studies of tissue-based immunity, including vaccine responses, infections, autoimmunity and cancer.

中文翻译：

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人体B细胞克隆分布图集

B细胞反应可导致克隆扩增，并可发生在多种组织中。为了定义B细胞克隆在体内的分布方式，我们对933,427个B细胞克隆世系进行了测序，并将其映射到六个人体器官捐献者中的八个不同的解剖区室中。我们显示，大型B细胞克隆可分为两个广泛的网络-一个跨越血液，骨髓，脾脏和肺部，而另一个则局限于胃肠道（GI）道内的组织（空肠，回肠和结肠）。值得注意的是，胃肠道克隆在肠道的不同部分之间显示出广泛的序列变异共享，并且具有更高的体细胞超突变频率，表明克隆扩增和选择的广泛轮次序列。我们的发现提供了B细胞克隆谱系，它们的特性和组织连接的解剖图谱。