Flow cytometry is a technology providing multiparametric analysis of single cells or other suspension particles. High-throughput (HT) flow cytometry has become an attractive screening platform for drug discovery. In this review, we highlight the recent HT flow cytometry applications, and then focus on HT flow cytometry deployment at AstraZeneca (AZ). Practical considerations for successful HT flow cytometry assay development and screening are provided based on experience from four project case studies at AZ. We provide an overview of the scientific rationale, explain why HT flow cytometry was chosen and how HT flow cytometry assays deliver new ways to support the drug discovery process.

流式细胞术是一种提供单细胞或其他悬浮颗粒多参数分析的技术。高通量（HT）流式细胞仪已成为用于药物发现的有吸引力的筛选平台。在这篇综述中，我们重点介绍了最新的HT流式细胞术应用，然后重点介绍了在阿斯利康（AZ）进行的HT流式细胞术的部署。基于AZ四个项目案例研究的经验，提供了成功进行HT流式细胞术测定和开发的实践考虑。我们提供了科学依据的概述，解释了为什么选择HT流式细胞术以及HT流式细胞术如何提供新方法来支持药物发现过程。