当前位置: X-MOL 学术Nat. Mater. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Long-lived force patterns and deformation waves at repulsive epithelial boundaries
Nature Materials ( IF 41.2 ) Pub Date : 2017-09-11 , DOI: 10.1038/nmat4972
Pilar Rodríguez-Franco , Agustí Brugués , Ariadna Marín-Llauradó , Vito Conte , Guiomar Solanas , Eduard Batlle , Jeffrey J. Fredberg , Pere Roca-Cusachs , Raimon Sunyer , Xavier Trepat

For an organism to develop and maintain homeostasis, cell types with distinct functions must often be separated by physical boundaries. The formation and maintenance of such boundaries are commonly attributed to mechanisms restricted to the cells lining the boundary. Here we show that, besides these local subcellular mechanisms, the formation and maintenance of tissue boundaries involves long-lived, long-ranged mechanical events. Following contact between two epithelial monolayers expressing, respectively, EphB2 and its ligand ephrinB1, both monolayers exhibit oscillatory patterns of traction forces and intercellular stresses that tend to pull cell–matrix adhesions away from the boundary. With time, monolayers jam, accompanied by the emergence of deformation waves that propagate away from the boundary. This phenomenon is not specific to EphB2/ephrinB1 repulsion but is also present during the formation of boundaries with an inert interface and during fusion of homotypic epithelial layers. Our findings thus unveil a global physical mechanism that sustains tissue separation independently of the biochemical and mechanical features of the local tissue boundary.



中文翻译:

排斥力上皮边界处的长寿命力模式和形变波

为了使生物体发展并维持体内稳态,具有不同功能的细胞类型通常必须通过物理边界加以分隔。这种边界的形成和维持通常归因于限于限定边界的细胞的机制。在这里,我们表明,除了这些局部亚细胞机制外,组织边界的形成和维持还涉及长寿命,长距离的机械事件。在分别表达EphB2及其配体ephrinB1的两个上皮单层之间接触之后,两个单层都表现出牵引力和细胞间应力的振荡模式,这些模式倾向于使细胞-基质粘附远离边界。随着时间的流逝,单分子层会阻塞,并伴随着变形波的出现,这些变形波从边界传播开来。这种现象不是EphB2 / ephrinB1排斥的特有现象,在具有惰性界面的边界形成过程中以及同型上皮层融合过程中也存在。因此,我们的发现揭示了一种全球性的物理机制,可维持组织分离,而与局部组织边界的生化和机械特征无关。

更新日期:2017-09-12
down
wechat
bug