Alkynes are a key component of click chemistry and used for a wide variety of applications including bioconjugation, selective tagging of protein modifications, and labeling of metabolites and drug targets. However, challenges still exist for detecting alkynes because most 1,2,3-triazole products from alkynes and azides do not possess distinct intrinsic properties that can be used for their facile detection by either fluorescence or mass spectrometry. To address this critical need, a novel brominated coumarin azide was used to tag alkynes and detect alkyne-conjugated biomolecules. This tag has several useful properties: first, it is fluorogenic and the click-chemistry products are highly fluorescent and quantifiable; second, its distinct isotopic pattern facilitates identification by mass spectrometry; and third, its click-chemistry products form a unique pair of reporter ions upon fragmentation that can be used for the quick screening of data. Using a monoclonal antibody conjugated with alkynes, a general workflow has been developed and examined comprehensively.

中文翻译：

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质谱法同时测定荧光和同位素特征，通过点击化学与溴化香豆素叠氮化物通过点击化学检测炔烃

炔烃是点击化学的关键组成部分，可用于多种应用，包括生物缀合，蛋白质修饰的选择性标记以及代谢物和药物靶标的标记。然而，对于炔烃的检测仍然存在挑战，因为来自炔烃和叠氮化物的大多数1,2,3-三唑产物不具有可用于通过荧光或质谱法轻松检测的独特内在特性。为了解决这一关键需求，使用了一种新型的溴化香豆素叠氮化物来标记炔烃并检测与炔烃偶联的生物分子。该标签具有几个有用的特性：首先，它具有荧光性，点击化学产物具有很高的荧光性和可量化性。其次，其独特的同位素模式便于质谱鉴定。第三，其点击化学产品在断裂时会形成一对独特的报告离子，可用于快速筛选数据。使用与炔烃偶联的单克隆抗体，已经开发并全面检查了一般工作流程。