Biotechnology Advances ( IF 16.0 ) Pub Date : 2017-07-16 , DOI: 10.1016/j.biotechadv.2017.07.005 Jyun-Liang Lin , James M. Wagner , Hal S. Alper
Within the Design-Build-Test Cycle for strain engineering, rapid product detection and selection strategies remain challenging and limit overall throughput. Here we summarize a wide variety of modalities that transduce chemical concentrations into easily measured absorbance, luminescence, and fluorescence signals. Specifically, we cover protein-based biosensors (including transcription factors), nucleic acid-based biosensors, coupled enzyme reactions, bioorthogonal chemistry, and fluorescent and chromogenic dyes and substrates as modalities for detection. We focus on the use of these methods for strain engineering and enzyme discovery and conclude with remarks on the current and future state of biosensor development for application in the metabolic engineering field.
中文翻译:
高通量代谢物检测工具:代谢工程和定向进化应用
在应变工程的“设计-建造-测试”周期中,快速的产品检测和选择策略仍然具有挑战性,并限制了总产量。在这里,我们总结了将化学浓度转换为易于测量的吸光度,发光和荧光信号的多种方式。具体来说,我们涵盖了基于蛋白质的生物传感器(包括转录因子),基于核酸的生物传感器,偶联酶反应,生物正交化学以及荧光和生色染料以及底物作为检测方式。我们专注于这些方法在菌株工程和酶发现中的使用,并以有关在代谢工程领域中应用的生物传感器开发的当前和未来状态作为总结。