Fish protein hydrolysates are an important source of antioxidant peptides. Electrically driven membrane fractionation called electrodialysis with filtration membrane (EDFM) is a separation technology based on molecular charge and mass, which can fractionate active peptides from complex hydrolysates. This work aimed to evaluate the feasibility of sequential EDFM process for separation of cationic (CP) and anionic (AP) peptides from rainbow trout frame protein hydrolysate, and determine their antioxidant properties. The concentrations of CP and AP increased in the recovery solution, reaching 156 and 85 μg/mL, respectively, after 4-hour treatment, with migration rates of 19.55 ± 2.19 and 10.94 ± 0.39 g/m² h. The CP separation was approximately 50% energy efficient than AP. Both CP and AP fractions were enriched with peptides with DPPH and ABTS radical scavenging properties. The results showed that two-step EDFM process is feasible for recovery and concentration of antioxidant peptides from rainbow trout protein hydrolysate.

Industrial relevance

The electro-membrane fractionation developed in this study is a two-step process, which is able to selectively separate antioxidant peptides from enzymatic protein hydrolysates based on charge and size. With this particular raw material as the feed, we have shown that using this approach lead to the highest peptide migration rate and a significant improvement in antioxidant activities of both peptide fractions. In addition, this technique is very selective, and environmental friendly as it requires no use of solvent and consumes less energy compared to conventional chromatographic techniques, and thus can be used as a green technology for the fractionation of bioactive peptides from a complex mixture of protein hydrolysates.

中文翻译：

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虹鳟鱼的蛋白质水解物的抗氧化肽的电膜分馏（虹鳟）副产物

鱼蛋白水解物是抗氧化剂肽的重要来源。电动膜分离技术称为带过滤膜的电渗析（EDFM），是一种基于分子电荷和质量的分离技术，可以从复杂的水解物中分离出活性肽。这项工作旨在评估从虹鳟鱼骨架蛋白水解物中分离阳离子（CP）和阴离子（AP）肽的顺序EDFM工艺的可行性，并确定其抗氧化性能。处理4小时后，回收溶液中CP和AP的浓度增加，分别达到156和85μg/ mL，迁移率为19.55±2.19和10.94±0.39 g / m ² H。CP分离的能量效率比AP大约高50％。CP和AP馏分均富含具有DPPH和ABTS自由基清除特性的肽。结果表明，两步法EDFM工艺对于从虹鳟鱼蛋白水解物中回收和浓缩抗氧化肽是可行的。

行业相关性

在这项研究中开发的电膜分级分离是一个两步过程，它能够根据电荷和大小从酶促蛋白水解物中选择性地分离出抗氧化剂肽。使用这种特殊的原料作为饲料，我们已经表明，使用这种方法可导致最高的肽迁移速率，并显着提高两个肽级分的抗氧化活性。此外，该技术具有很高的选择性，并且对环境友好，因为与传统的色谱技术相比，它不需要使用溶剂并且消耗的能量更少，因此可以用作绿色技术，用于从复杂的蛋白质混合物中分离生物活性肽。水解物。