当前位置: X-MOL 学术Cell Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor
Cell Research ( IF 44.1 ) Pub Date : 2017-08-29 , DOI: 10.1038/cr.2017.111
Lijie Wang , Wei Xue , Lei Yan , Xiaosa Li , Jia Wei , Miaomiao Chen , Jing Wu , Bei Yang , Li Yang , Jia Chen

Base editors (BEs) have been recently developed by combining the APOBEC (apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like)/AID (activation-induced deaminase) cytidine deaminase family members1 with the CRISPR/Cas9 system to perform targeted C-to-T base editing2, 3, 4, 5,6,7,8. Mechanistically, Cas9 variant-fused APOBEC/AID is directed to target site by sgRNA, introducing C-to-T substitution at the single-base level2,3,4. Compared to earlier generations of BEs (BE1 and BE2), the latest BE3 achieved much higher base editing frequencies by substituting catalytically-dead Cas9 (dCas9) with Cas9 nickase (nCas9)2.

中文翻译:

通过共表达游离尿嘧啶DNA糖基化酶抑制剂增强碱基编辑

最近通过将APOBEC(载脂蛋白B mRNA编辑酶,催化多肽样)/ AID(激活诱导的脱氨酶)胞苷脱氨酶家族成员1与CRISPR / Cas9系统结合来开发碱基编辑器(BEs),以进行靶向C-to- T基本编辑2、3、4、5、6、7、8。从机制上讲,与Cas9变体融合的APOBEC / AID通过sgRNA定向到目标位点,在单碱基水平2、3、4上引入了C至T取代。与前几代BE(BE1和BE2)相比,最新的BE3通过用Cas9切口酶(nCas9)2取代死催化剂的Cas9(dCas9)实现了更高的碱基编辑频率。
更新日期:2017-08-31
down
wechat
bug