Pseudomonas aeruginosa is an opportunistic pathogen that can be very hard to treat because of high resistance to different antibiotics and alternative treatment regimens are greatly needed. An alternative or a complement to traditional antibiotic is to inhibit virulence of the bacteria. The salicylidene acylhydrazide, INP0341, belongs to a class of compounds that has previously been shown to inhibit virulence in a number of Gram-negative bacteria. In this study, the virulence blocking effect of INP0341 on P. aeruginosa was studied in vitro and in vivo. Two important and closely related virulence system were examined, the type III secretion system (T3SS) that translocates virulence effectors into the cytosol of the host cell to evade immune defense and facilitate colonization and the flagella system, needed for motility and biofilm formation. INP0341 was shown to inhibit expression and secretion of the T3SS toxin exoenzyme S (ExoS) and to prevent bacterial motility on agar plates and biofilm formation. In addition, INP0341 showed an increased survival of P. aeruginosa-infected mice. In conclusion, INP0341 attenuates P. aeruginosa virulence.

中文翻译：

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水杨基酰肼INP0341在体内和体外减弱铜绿假单胞菌的毒力

铜绿假单胞菌是一种机会病原体，由于对不同抗生素的高度耐药性，因此很难治疗，因此非常需要替代治疗方案。传统抗生素的替代品或补充品是抑制细菌的毒性。亚水杨酰基酰肼INP0341属于一类化合物，以前已显示出它能抑制多种革兰氏阴性细菌的致病性。在这项研究中，INP0341对铜绿假单胞菌的毒力阻断作用进行了体外和体内研究。检查了两个重要且密切相关的毒力系统，即III型分泌系统（T3SS），该系统将毒力效应子转移到宿主细胞的胞质溶胶中，可以逃避免疫防御并促进定殖和鞭毛系统，这是运动性和生物膜形成所必需的。已证明INP0341抑制T3SS毒素外切酶S（ExoS）的表达和分泌，并防止琼脂平板上的细菌运动和生物膜形成。此外，INP0341显示铜绿假单胞菌感染小鼠的存活率提高。总之，INP0341减弱了铜绿假单胞菌的毒力。