G protein-coupled receptors (GPCRs) are major signal recognition and transmission units in the plasma membrane. The interaction of activated and phosphorylated GPCRs with the multifunctional adaptor proteins β-arrestins (βarrs) is crucial for regulation of their signaling and functional outcomes. Over the past few years, a range of structural, biochemical, and cellular studies have revealed novel insights into GPCR–βarr interaction and signaling. Some of these findings have come as a surprise and therefore have the potential to significantly refine the conceptual framework of the GPCR–βarr system. Here we discuss these recent advances with particular emphasis on biphasic GPCR–βarr interaction, the formation of GPCR–G-protein–βarr supercomplexes, and receptor-specific conformational signatures in βarrs. We also underline the emerging research areas that are likely to be at the center stage of investigations in the coming years.

G蛋白偶联受体（GPCR）是质膜中的主要信号识别和传递单位。活化的和磷酸化的GPCR与多功能衔接蛋白β-arrestin（βarrs）的相互作用对于调节其信号传导和功能结果至关重要。在过去的几年中，一系列的结构，生化和细胞研究揭示了对GPCR-βarr相互作用和信号传导的新颖见解。其中一些发现令人惊讶，因此有可能极大地改善GPCR-βarr系统的概念框架。在这里，我们将讨论这些最新进展，特别着重于双相GPCR-βarr相互作用，GPCR-G蛋白-βarr超复合物的形成以及βarrs中受体特异性的构象特征。