The amide group modified silica materials are popular stationary phases for hydrophilic interaction liquid chromatography (HILIC). Meanwhile, surface-confined imidazolium ionic liquids (ILs) have been proved to be useful HILIC stationary phases and possess many unique properties. In this study, the synthesis of an amide-functionalized imidazolium IL was conducted which was then bonded onto silica surface to obtain a novel imidazolium-embedded amide stationary phase for HILIC. The combination of the amide group and imidazolium IL moiety might bring some advantages in selectivity or retention and therefore extended its applications. After characterizing the prepared IL and the resulting modified silica materials, the chromatographic performance and separation selectivity of the packed column were evaluated and compared with a commercial amide column. Then, the retention behavior was investigated through observing the retention factors at different chromatographic conditions using a wide range of compounds. Exceptionally, the prepared amide IL column exhibited superior separation performance towards complex samples such as flavonoids mixture, soybean flavonoids and human urine. All the results indicated that the novel amide IL column possessed an anion-exchange/HILIC mixed-mode retention mechanism and could be useful in the sample analysis as a promising candidate for HILIC stationary phase.

酰胺基改性的二氧化硅材料是亲水相互作用液相色谱法（HILIC）常用的固定相。同时，已证明表面受限的咪唑鎓离子液体（IL）是有用的HILIC固定相，并具有许多独特的性能。在这项研究中，进行了酰胺官能化的咪唑鎓IL的合成，然后将其键合到二氧化硅表面上以获得用于HILIC的新型咪唑嵌入式酰胺固定相。酰胺基和咪唑类IL部分的组合可能在选择性或保留方面带来一些优势，因此扩展了其应用范围。在表征了制备的IL和所得的改性二氧化硅材料后，评估了填充柱的色谱性能和分离选择性，并将其与市售的酰胺柱进行了比较。然后，通过使用各种化合物观察不同色谱条件下的保留因子来研究保留行为。例外地，所制备的酰胺类IL色谱柱对复杂的样品（如类黄酮混合物，大豆类黄酮和人尿）具有优异的分离性能。所有结果表明，新型酰胺IL色谱柱具有阴离子交换/ HILIC混合模式保留机制，可作为HILIC固定相的有希望的候选物用于样品分析。大豆类黄酮和人尿。所有结果表明，新型酰胺IL色谱柱具有阴离子交换/ HILIC混合模式保留机制，可作为HILIC固定相的有希望的候选物用于样品分析。大豆类黄酮和人尿。所有结果表明，新型酰胺IL色谱柱具有阴离子交换/ HILIC混合模式保留机制，可作为HILIC固定相的有希望的候选物用于样品分析。