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  • Core-shell structurized Fe3O4@C@MnO2 nanoparticles as pH-responsive T1-T2* dual-modal contrast agents for tumor diagnosis
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-16
    beichen duan, Dongdong Wang, huihui wu, pengping xu, Peng Jiang, Guoliang Xia, Zhenbang Liu, Haibao Wang, Zhen Guo, Qianwang Chen

    Biocompatible core-shell Fe3O4@C@MnO2 nanoparticles (named as FOCMO NPs) with an average size at 130 nm prepared through an effortless yet efficient strategy were employed as pH-activatable T1-T2* dual-modality magnetic resonance imaging (MRI) contrast agents (CAs). The release rate of Mn ions in acidic PBS (pH = 5.0) was approximately ten times to that under condition with pH value of 7.4. Benefiting from excellent acid responsiveness, which facilitates the release of ions from FOCMO NPs at tumor region with acidic microenvironment and organelles, the diagnosis accuracy was commendably improved. After intravenous injection of FOCMO NPs, an efficiently intensive contrast in tumors are realized with a distinct enhancement of 127% in T1 MRI signal 24 h after the administration. Moreover, a significant decreasement of 71% are witnessed in T2 MRI signal. Those demonstrated that FOCMO NPs can achieve the purpose of positive/negative MRI simultaneously. Furthermore, obtained FOCMO NPs showed great hemocompatibility and negligible toxicity.

    更新日期:2018-07-16
  • Placental barrier-on-a-chip: modeling placental inflammatory responses to bacterial infection
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-16
    Jianhua Qin, Yujuan Zhu, Fangchao Yin, Hui Wang, Li Wang, Jingli Yuan

    Placental inflammation, as a recognized cause of preterm birth and neonatal mortality, displays extensive placental involvement or damage with the presence of organisms. The inflammatory processes are complicated and tightly associated with increased inflammatory cytokine levels and innate immune activation. However, the deep study of the underlying mechanisms was limited by conventional cell and animal models due to great variations in the architecture and function of placenta. Here, we established a microengineered model of human placental barrier on the chip and investigated the associated inflammatory responses to bacterial infection. The multilayered design of the microdevice mimicked the microscopic structure in the fetal-maternal interfaces of human placenta, and the flow resembled the dynamic environment in the mother’s body. Escherichia coli (E. coli), one of the predominant organisms found in fetal organs, were applied to the maternal side, modeling acute placental inflammation. The data demonstrated the complex responses including the increased secretion of inflammatory cytokines by trophoblasts and the adhesion of maternal macrophages following bacterial infection. Particularly, transplacental communication was observed between two placental cells, and implied the potential role of trophoblast in fetal inflammatory response syndrome in clinic. These complex responses are of potential significance to placental dysfunctions, even abnormal fetal development and preterm birth. Collectively, placental barrier-on-a-chip microdevice presents a simple platform to explore the complicated inflammatory responses in human placenta, and might help our understanding of the mechanisms underlying reproductive diseases.

    更新日期:2018-07-16
  • Red Blood Cell-Shaped Microparticles with a Red Blood Cell Membrane Demonstrate Prolonged Circulation Time in Blood
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-13
    Koichiro Hayashi, Shota Yamada, Wataru Sakamoto, Eri Usugi, Masatoshi Watanabe, Toshinobu Yogo
    更新日期:2018-07-14
  • Effects of Molecular Weight and Concentration of Poly(Acrylic Acid) on Biomimetic Mineralization of Collagen
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-13
    Yipin Qi, Zhou Ye, Alex Fok, Brian N. Holmes, Montserrat Espanol, Maria-Pau Ginebra, Conrado Aparicio
    更新日期:2018-07-14
  • 更新日期:2018-07-12
  • Collagen Fiber Orientation Regulates 3D Vascular Network Formation and Alignment
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-11
    Michael G. McCoy, Jane M. Wei, Siyoung Choi, Julian Palacios Goerger, Warren Zipfel, Claudia Fischbach
    更新日期:2018-07-12
  • Optimization of Amphiphilic Miktoarm Star Copolymers for Anti-Cancer Drug Delivery
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-12
    Mingqi Wang, Xiaolong Zhang, Han Peng, Mingkui Zhang, Xianshuo Zhang, Zhe Liu, Liwei Ma, Hua Wei

    The preparation of various types of miktoarm star polymers with precisely controlled structures (A2B, ABC, AB2C2, etc.) has made significant progress due to the considerable advances in the synthetic strategies including multi-step protections/deprotections, orthogonality, and integration of different polymerization techniques. However, compared to the well-developed synthesis methodologies, the adoption of amphiphilic miktoarm star polymers for drug delivery applications remains relatively unexplored. To elucidate the structure-property relationship of amphiphilic miktoarm star polymers as drug delivery vehicles, we prepared four different amphiphilic miktoarm star copolymers with the respectively identical molecular weights (MWs) of hydrophilic and hydrophobic moieties but different star structures by protection/deprotection strategies, and a combination of ring-opening polymerization (ROP) of ε-caprolactone (ε-CL) and atom transfer radical polymerization (ATRP) of oligo (ethylene glycol) monomethyl ether methacrylate (OEGMA). Further screening of an optimal formulation for anti-cancer drug delivery by the stability of micelles, in vitro drug loading capacity, drug release properties, cellular uptake efficacy, and in vitro cytotoxicity of doxorubicin (DOX)-loaded micelles showed that PCL3POEGMA1 micelles possessed the lowest critical micelle concentration (CMC), the highest drug loading content (DLC), and enhanced therapeutic efficiency for DOX release of all the synthesized four star copolymer constructs. This study thus provides preliminary guidelines for rational design and development of amphiphilic miktoarm star polymers toward enhanced anti-cancer drug delivery.

    更新日期:2018-07-12
  • Toll-like receptor 2-dependent NF-κB/AP-1 activation by damage-associated molecular patterns adsorbed on polymeric surfaces
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-11
    Laura McKiel, Lindsay Fitzpatrick

    The foreign body reaction is a chronic inflammatory response to an implanted biomaterial that ultimately leads to fibrous encapsulation of the implant. It is widely accepted that the host response to implanted biomaterials is largely dependent on the species and conformations of proteins adsorbed onto the material surface, due to the adsorbate’s role in mediating cellular interactions with the implanted material. While the cellular response to adsorbed serum-derived proteins has been studied extensively, the presence of endogenous, matrix- and cell-derived mediators of inflammation within the adsorbed protein layer and their impact on cell-material interactions is not well understood. Damage associated molecular patterns (DAMPs) are endogenous ligands released by stressed or damaged tissues to stimulate sterile inflammatory responses via Toll-like receptors (TLRs) and other pattern recognition receptors. The present study investigated the potential role of tissue-derived, pro-inflammatory stimuli in macrophage responses to biomaterials using cell lysate as a complex source of cell-derived DAMPs and poly(methyl methacrylate) (PMMA) and polydimethylsiloxane (PDMS) films as model biomaterials. We show that lysate-adsorbed PMMA and PDMS surfaces strongly induced NF-κB/AP-1 transcription factor activity and pro-inflammatory cytokine secretion in the RAW-Blue macrophage cell line, compared to serum-adsorbed surfaces. Lysate-dependent NF-κB/AP-1 activation and cytokine expression was strongly attenuated by TLR2 neutralizing antibodies, while TLR4 inhibition resulted in a modest reduction. These data suggest that DAMPs, in their adsorbed conformations on material surfaces, may play a significant role in macrophage activation through TLR signalling, and that TLR pathways, particularly TLR2, merit further investigation as potential therapeutic targets to modulate host responses to implanted biomaterials.

    更新日期:2018-07-12
  • WETTABILITY STUDY ON NATURAL RUBBER SURFACES FOR APPLICATIONS AS BIOMEMBRANES
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-10
    Rodney Marcelo do Nascimento, Stella M.M. Ramos, Ivan Helmuth Bechtold, Antonio Carlos Hernandes

    This manuscript reports an experimental study on surfaces of natural rubber membranes modified by incorporation of calcium phosphate particles. In particular, we focused on the wettability, a subject for biological aspects. Five surfaces of natural rubber (NR) membranes (pure, polymer-bioceramic composite (NR-CaP) and three modified surfaces subjected to a simulated body fluid (NR-SBF)) were produced and characterized by confocal Raman-spectroscopy, AFM, SEM and XPS and the results were correlated with the wetting properties. Seven liquids (water, formamide, di-iodomethane, ethylene glycol, hexadecane, simulated body fluid and human blood droplets) were used in different experimental sections. Static and dynamic contact angle measurements were conducted to obtain the solid-liquid tensions, work of adhesion and depinning forces. The incorporation of CaP particles in the polymer decreases the roughness and increases the interfacial adhesion, and there was no dependence between the morphology and equilibrium contact line. The hydrophobic state of the NR surfaces is preserved. After exposure to a biological environment, the NR surfaces were chemically modified increasing blood wettability and decreasing the negative surface charges and the contact angle to values close to those associated with protein adsorption and cell adhesion. Therefore, opening possibilities for applications of these materials as biomembranes. On the other hand, the concepts applied, regarding different wettability aspects, should enable the evaluation of biomaterial surfaces and provide new insights allowing a better understanding of body fluid-material interfaces.

    更新日期:2018-07-12
  • MCM-41 Nanoparticles for Brain Delivery: Better Choline-Esterase and Amyloid Formation Inhibition with Improved Kinetics
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-09
    Pawan Kumar Pandey, Ashok K Sharma, Sarita Rani, Gaurav Mishra, Kandasamy Gopal, Ajit K Patra, Monika Rana, Anuj Sharma, Awesh Kumar Yadav, Umesh Gupta

    Present study was aimed to deliver a low bioavailability drug, rivastigmine hydrogen tartrate (RTG) to the brain through its encapsulation in Mesoporous silica nanoparticles (MSNs) and targeted to amyloid inhibition in brain. MSNs were characterized for size, zeta potential, and drug entrapment using SEM, TEM, HR-TEM, FT-IR and PXRD. Drug-loaded MSNs were assessed for in vitro release kinetics, ex vivo followed by animal studies. The average size of the prepared blank (MCM-41B) and drug-loaded MSNs (MCM-41L) was 114 ± 2.0 and 145 ± 0.4 nm with the zeta potential of approximately −43.5 ± 1.1 and −37.6 ± 1.4 mV, respectively. MCM-41L exhibited an average entrapment efficiency of 88%. In vitro release studies exhibited early surge followed by a sluggish persistent or constant release (biphasic pattern). Hemolytic studies proved that the developed MCM-41L NPs are less hemolytic compared to RTG. A reduced ThT fluorescence was observed with MCM-41L compared to MCM-41B and RTG in the amyloid inhibition studies. A significant (p<0.05) inhibition of AChE (acetycholinesterase) was observed for MCM-41L (80±4.98%) RTG (62±3.25%) and MCM-41B (54±4.25%). In vivo pharmacokinetics in Wistar rats revealed that the AUC and mean residence time (MRT) for MCM-41L was sustained and significantly higher (p<0.05) (780 ± 3.30 ng/L; 5.49 ± 0.25 h) compared to RTG solution (430±3.50 ng/L; 0.768 ± 0.17 h). Similarly, the half-life was found to be significantly higher in case of MCM-41L. The promising result was brain delivery of RTG in Wistar rats which was enhanced almost 127 folds in vivo, using MCM-41L nanoparticles. MCM-41L nanoparticles effectively enhanced the bioavailability of RTG. Conclusively, these can be used for the administration of RTG and other related low bioavailability drugs for improved brain delivery.

    更新日期:2018-07-10
  • High-Throughput Screening of Rat Mesenchymal Stem Cell Behavior on Gradient TiO2 Nanotubes
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-09
    Ping Mu, Yanran Li, Yanmei Zhang, Yun Yang, Ren Hu, Xulin Zhao, Anhua Huang, Ruofan Zhang, Xiangyang Liu, Qiaoling Huang, Changjian Lin
    更新日期:2018-07-09
  • Surface severe plastic deformation of an orthopedic Ti-Nb-Sn alloy induces unusual precipitate remodeling and supports stem cell osteogenesis through Akt signaling
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-09
    Sumit Bahl, Sai R K Meka, Satyam Suwas, Kaushik Chatterjee

    This work presents a strategy to augment the bioactivity of a new-generation metastable β Ti-Nb-Sn alloy through surface severe plastic deformation. Foremost, the alloy was strengthened by precipitation of α phase using a well-designed thermo-mechanical processing route. Subsequently, the surface of the aged alloy was subjected to severe plastic deformation via surface mechanical attrition treatment (SMAT). Upon SMAT, a unique phenomenon of strain-induced precipitate coarsening was observed. A possible mechanism is proposed wherein the precipitates first dissolve due to significant slip transfer across the α/β interface followed by reprecipitation along the other precipitates thereby leading to coarsening. Coarsening of the precipitates abrogated the strengthening caused by plastic deformation as a result of which the hardness did not increase significantly after SMAT in sharp contrast to other alloys. SMAT led to a decrease in the attachment of human mesenchymal stem cells due to an increase in the roughness-mediated surface hydrophobicity. On the other hand, an increase in the roughness led to the formation of more number of focal adhesions. This in turn enhanced the proliferation rate and more importantly, osteogenic differentiation of stem cells. Detailed investigation into the underlying mechanism revealed that an increase in focal adhesions activated the Akt mediated mechano-transduction signaling pathway that enhanced the osteogenic differentiation. In summary, the potential of surface severe plastic deformation to impart bioactivity to the next-generation of orthopedic β Ti alloys is underscored in this work.

    更新日期:2018-07-09
  • 3D printing of a tyramine hyaluronan derivative with double gelation mechanism for independent tuning of shear thinning and post-printing curing
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-09
    Dalila Petta, Dirk W. Grijpma, Mauro Alini, David Eglin, Matteo D'Este

    Biofabrication via three-Dimensional Printing (3DP) is expanding our capabilities of producing tissue engineering constructs for regenerative medicine, personalized medicine, and engineered tissue models of disease and diagnostics. Hydrogel-based materials for extrusion-based printing have been introduced, nevertheless it is still challenging to combine into a single biomaterial all their requirements of an ink. These inks need to flow for extrusion under low shear, yet have immediate shape retention after deposition, provide a biochemical environment similar to physiological extracellular matrix, and a curing mechanism avoiding cell damage. This work introduces a simple and versatile tyramine-modified hyaluronan material (HA-Tyr) for extrusion-based printing, featured by: i) single component yet 2 distinct crosslinking mechanisms, allowing ii) shear-thinning tuning independently of the post-printing curing; iii) no rheological additives or sacrificial components; iv) curing with visible light for shape stability; v) possibility to post functionalize; vi) preservation of hyaluronan structure owing to low modification degree. The ink is based on a hydroxyphenol hyaluronan derivative, where the shear thinning properties are determined by the enzymatic crosslinking, while the final shape fixation is achieved with visible light in presence of Eosin Y as photosensitizer. The two crosslinking mechanisms are totally independent. A universal rheologically measurable parameter giving a quantitative measure of the "printability" was introduced and employed for identifying best printability range within the parameter space in a quantitative manner. 3DP constructs were post functionalized and cell-laden constructs produced. Due to its simplicity and versatility, HA-Tyr can be used for producing a wide variety of 3D printing constructs for tissue engineering applications.

    更新日期:2018-07-09
  • 更新日期:2018-07-08
  • Separable Microneedles for Near-Infrared Light-Triggered Transdermal Delivery of Metformin in Diabetic Rats
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-06
    Yang Zhang, Danfeng Wang, Mengyue Gao, Bin Xu, Jiangying Zhu, Weijiang Yu, Depeng Liu, Guohua Jiang
    更新日期:2018-07-08
  • 更新日期:2018-07-08
  • Supramolecular Threading of Peptide Hydrogel Fibrils
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-05
    Niklas Hauptstein, Luis M. De Leon-Rodriguez, Alok K. Mitra, Yacine Hemar, Iman Kavianinia, Na Li, Valeria Castelletto, Ian W. Hamley, Margaret A. Brimble
    更新日期:2018-07-08
  • Blue-Light -Activated Nano-TiO2@PDA for Highly Effective and Nondestructive Tooth Whitening
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-05
    Feng Zhang, Chongxue Wu, Ziyu Zhou, Jiaolong Wang, Weiwei Bao, Lina Dong, Zihao Zhang, Jing Ye, Lan Liao, Xiaolei Wang
    更新日期:2018-07-08
  • Establishment of an ex Vivo Model of Nonalcoholic Fatty Liver Disease Using a Tissue-Engineered Liver
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-05
    Qiao Wu, Juan Liu, Lijin Liu, Yu Chen, Jie Wang, Ling Leng, Qunfang Yu, Zhongping Duan, Yunfang Wang
    更新日期:2018-07-08
  • Silk fibroin porous scaffolds loaded with a slow-releasing hydrogen sulfide agent (GYY4137) for applications of tissue engineering
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-06
    Rosasilvia Raggio, Walter Bonani, Emanuela Callone, Sandra Dire, Laura Gambari, Francesco Grassi, Antonella Motta

    Hydrogen sulfide (H2S) is a physiological gasotransmitter known to possess a regulatory role in several tissues, including bone. The exogenous administration by injection of solutions of H2S-releasing compounds, e.g. GYY4137, has been previously investigated as a novel therapeutic approach for the treatment of bone diseases. Here, GYY4137 was embedded into fibroin sponges, previously shown to be suitable as scaffolds for bone, thanks to their biocompatibility, scalable porous structure and biodegradability rate. Fibroin porous scaffolds were produced by solvent casting and particulate leaching method, and GYY4137 was successively incorporated by using dimethyl sulfoxide (DMSO) as vehicle. The process used to produce GYY4137-loaded scaffolds allowed to incorporate different controlled amounts of GYY4137 into fibroin matrices. The loading process preserved the properties of the system components in the final products, as assessed by SEM, FT-IR, NMR and different thermal analyses techniques. Release of H2S from GYY4137 incorporated into the scaffolds was monitored upon incubation in saline solution at physiological pH: H2S-release kinetic was found to be dependent on the amount of GYY4137. To ensure biocompatibility, mouse fibroblasts and human primary bone marrow stromal cells were seeded onto scaffolds, and short-term viability assays were performed. Results showed that GYY4137-loaded scaffold did not induce cytotoxicity in any of the cell type tested. Our findings demonstrate that embedding an H2S-releasing donor in silk fibroin scaffold is a suitable strategy to achieve a long-lasting release of H2S that preserves cell viability and allows local delivery at sites of tissue injury.

    更新日期:2018-07-08
  • Amyloid Fibrils with Positive Charge Enhance Retroviral Transduction in Mammalian Cells
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-06
    Sneha Kirti, Komal Patel, Subhadeep Das, Paresh Shrimali, Sonali Samanta, Rakesh Kumar, Debdeep Chatterjee, Dhiman Ghosh, Ashutosh Kumar, Prakriti Tayalia, Samir K. Maji

    Amyloid fibrils are cross-β-sheet rich protein/peptide fibrils that are typically associated with neurodegenerative diseases such as Parkinson’s and Alzheimer’s disease. Recently, functional amyloids have been discovered where amyloids are implicated in performing normal physiological functions of the host organism rather than creating diseases. The ability of amyloids to interact with the cell membrane and other small biomolecules exhibits its great potential to be used as a biomaterial for cell adhesion and gene delivery system. Given the established ability of semen-derived amyloids to concentrate HIV in semen and that of charged polymers as an enhancer of retroviral gene transfer, we hypothesized that charged amyloid fibrils can augment virus-mediated delivery system. We show that amyloids of α-synuclein formed in the presence and absence of cationic polymers chitosan and amyloid of poly-L-lysine can interact with lentiviral particles and enhance transduction efficiency in cells. The amyloid nanofibrils increase transduction efficiency up to ~4 fold similar to widely used cationic polymer polybrene. This study shows that amyloid nanofibril scaffolds may be used as targeted gene delivery systems.

    更新日期:2018-07-08
  • Modular Assembly of a Conserved Repetitive Sequence in the Spider Eggcase Silk: from Gene to Fiber
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-06
    Jianming Chen, Jinlian Hu, Sono Sasaki, Kensuke Naka

    Spider silk features extraordinary toughness in combination with great biocompatibility and biodegradability, fascinating researchers to prepare artificial silk fibers inspired from the natural art of spinning. In addition to C- and N-terminal domain, a repeat unit from Lactrodectus Mactans spider eggcase silks displays substantial sequence conservation across species. Herein, we attempt to spin the engineered tubuliform spidroin 1 (eTuSp1) by microfluidics in a mode of modular assembly comprising the genetic construction, micellar formation, phase separation and further solidification. Based on the conserved gene sequence, a unique amphiphilic behavior was predicted and then verified by combined techniques of DLS, TEM and synchrotron radiation XRD to reveal the formation of micelle-like structure. Through the employ of biomimetic microfluidic devices, desolvation of eTuSp1 was simplified by the non-solvent induced phase separation in place of the conventional ions exchange and acidification. Both controlled by protein concentrations and flow rate ratios, silk fibers were assembled similar to these reported in other studies of spheres/spherical aggregates observed as intermediates. Due to the applied shear and elongational flow in microfluidic systems, these intermediates were forced to form fibrillar assemblies accompanied by the conformational transformation from α-helix to β-sheet. The resultant mechanical properties were investigated in response to the change of secondary structures and morphologie during spinning process. This work studies the sequence-structure-property relationship, providing comprehensive and systematic insight into the design rational on the preparation of artificial silk fibers from micro to macro.

    更新日期:2018-07-08
  • Advanced Silk Fibroin Biomaterials for Cartilage Regeneration
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-05
    Gu Cheng, Zahra Davoudi, Xin Xing, Xin Yu, Xin Cheng, Zubing Li, Hongbing Deng, Qun Wang

    Silk fibroin is regarded as a natural fibrous protein with tunable mechanical properties, acceptable biocompatibility and the favorable capability of enhancing attachment, proliferation and differentiation of chondrocytes. These properties make it suitable for the fabrication of scaffold and a broad range of silk fibroin-based biomaterials for cartilage regenerative therapy which can heal functional cartilage without scar tissue. It can be used as a single material for making different kinds of scaffold or as a composite with other types of biomaterials. Together with some growth factors, silk fibroin scaffolds can form a support for the growth and differentiation of seeding cells, such as chondrogenic lineage cells and mesenchymal stem cells. The recent advancements of silk fibroin in cartilage regeneration is summarized in this review. Furthermore, the manufacture methods of silk fibroin materials and their applications in the regeneration of cartilage were also discussed.

    更新日期:2018-07-08
  • Fabrication of Functional Biomaterial Microstructures by In situ Photopolymerization and Photodegradation
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-05
    Paige J LeValley, Ben Noren, Prathamesh Madhav Kharkar, April M. Kloxin, Jesse C Gatlin, John Oakey

    The in situ fabrication of poly(ethylene glycol) diacrylate (PEGDA) hydrogel microstructures within poly(dimethylsiloxane) (PDMS)-based microfluidic networks is a versatile technique that has enabled unique applications in biosensing, medical diagnostics, and the fundamental life sciences. Hydrogel structures have previously been patterned by the lithographic photopolymerization of PEGDA hydrogel forming solutions, a process that is confounded by oxygen-permeable PDMS. Here, we introduce an alternate PEG patterning technique that relies upon the optical sculpting of features by patterned light-induced erosion of photodegradable PEGDA deemed negative projection lithography. We quantitatively compared the hydrogel micropatterning fidelity of negative projection lithography to positive projection lithography, using traditional PEGDA photopolymerization, within PDMS devices. We found that the channel depth, the local oxygen atmosphere, and the UV exposure time dictated the size and resolution of hydrogel features formed using positive projection lithography. In contrast, negative projection lithography was observed to deliver high-resolution functional features with dimensions on the order of single micrometers enabled by its facilely controlled mechanism of feature formation that is insensitive to oxygen. Next, the utility of photodegradable PEGDA was further assessed by encapsulating or conjugating bioactive molecules within photodegradable PEG matrices to provide a route to the formation of complex and dynamically reconfigurable chemical microenvironments. Finally, we demonstrated that negative projection lithography enabled photopatterning of multilayered microscale objects without the need for precise mask alignment. The described approach for photopatterning high-resolution photolabile hydrogel microstructures directly within PDMS microchannels could enable novel microsystems of increasing complexity and sophistication for a variety of clinical and biological applications.

    更新日期:2018-07-08
  • Ultrasound technology for molecular imaging: From contrast agents to multimodal imaging
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-05
    Yue Li, Yuhao Chen, Meng Du, Zhi-Yi Chen

    Ultrasound (US) takes advantage of ultrasound contrast agents (UCAs) to further increase the sensitivity and specificity of monitoring in cellular levels which has a considerable effect on the modern molecular imaging field. Gas-filled microbubble (MB) in the bloodstream generate resonant volumetric oscillations in response to the rapid variations of acoustic pressure, which related to both the acoustics parameters of applied ultrasound and MBs’ physicochemical properties. Nanoscale UCAs have been developed and attracted much attention in the ability of detecting extravascular lesions. Ultrasound molecular assessment are achieved by binding disease-specific ligands to the surface of UCAs, which have been designed for targeting to tissue biomarkers in the area of interest, such as blood vessels, inflammation and thrombosis and so on. Additionally, the development of multimodal imaging technology is conducive to integration of the advantages of various imaging techniques to acquire additional diagnostic information. In this review paper, the present status and the critical issues for developing ultrasound contrast agent and multi-modal imaging application will be described. In this review paper, conventional microbubbles UCAs are introduced with their research material, diagnostic applications and intrinsic limitations firstly. Then recent progress in developing targeted UCA and phase-inversion contrast agent for diagnostic purposes will be described. Finally, we will state the present status and the critical issues for developing ultrasound-based multi-modal imaging application and summarize the existing challenges and prospects.

    更新日期:2018-07-08
  • Single-phase, Antibacterial Tri-Magnesium Phosphate Hydrate Coatings on Polyetheretherketone (PEEK) Implants by Rapid Microwave Irradiation Technique
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-05
    Prabaha Sikder, Corey R. Grice, Boren Lin, Vijay K Goel, Sarit B Bhaduri

    This paper reports the fabrication and evaluation of single-phase, silver-doped tri-magnesium phosphate hydrate (Ag-TMPH) nanosheet coatings on polyetheretherketone (PEEK), a well-known material used to fabricate orthopedic and spinal implants. While PEEK has better biomechanical compatibility with bone compared to metallic implants, it is also quite inert. Therefore, it is a common practice to coat PEEK implants with conventional calcium phosphates (CaPs) to enhance their osseointegration capability. As opposed to well-studied CaP compounds, relatively less-explored magnesium phosphates (MgPs) are also becoming interesting orthopedic biomaterials and is the prime focus in this research. The novel aspects of this paper are as follows. First, we report developing TMPH coatings within minutes with the help of microwave irradiation technology. Microwave irradiation plays an important role in the coating formation with accelerated kinetics. Scanning electron microscopy (SEM) confirmed the fabrication of ⁓650 nm thick TMPH coatings. The coatings resulted in sub-micron level surface roughness and in vitro cell studies confirmed enhanced MC3T3 cell adhesion within 4 hours on such surfaces. The coatings also resulted in significant apatite formation after immersing in simulated body fluid for 7 days. Second, multi-functionality was achieved by doping TMPH coatings with Ag, thus rendering the coatings antibacterial. The antibacterial properties were evaluated against two most common infection causing bacterial strains – gram-negative Escherichia coli and gram -positive Staphylococcus aureus. The results indicated good bacterial resistance and bactericidal properties of the Ag-TMPH coatings. Third, in spite of Ag doping, the single-phase nature of the coatings were retained (without forming composite systems) with the help of the low processing temperature of the microwave irradiation. The inductive coupled plasma technique confirmed that the doped single-phase TMPH coatings supported a uniform and controlled release of Ag+ ions over a period of 3 weeks. MTT assay evaluations and SEM micrographs confirmed no signs of cytotoxicity and healthy proliferation of cells in all cases. Quantitative real time PCR (qRT-PCR) indicated a significant rise in collagen (Col1) and osteocalcin (OCN) gene expression levels in the case of TMPH coated PEEK. Thus, microwave irradiation was successfully employed in forming multi-functional i.e. osseointegrable and antibacterial MgP coatings on PEEK.

    更新日期:2018-07-08
  • The Foreign Body Response Demystified
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-03
    Yashoda Chandorkar, Ravikumar K, Bikramjit Basu
    更新日期:2018-07-04
  • CAGW Modified Polymeric Micelles with Different Hydrophobic Cores for Efficient Gene Delivery and Capillary-like Tube Formation
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-03
    Xuefang Hao, Qian Li, Huaning Wang, Khan Muhammad, Jintang Guo, Xiangkui Ren, Changcan Shi, Shihai Xia, Wencheng Zhang, Yakai Feng
    更新日期:2018-07-04
  • Bioactive Self-Assembling Peptide Hydrogels Functionalized with Brain-Derived Neurotrophic Factor and Nerve Growth Factor Mimicking Peptides Synergistically Promote Peripheral Nerve Regeneration
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-03
    Changfeng Lu, Yu Wang, Shuhui Yang, Chong Wang, Xun Sun, Jiaju Lu, Heyong Yin, Wenli Jiang, Haoye Meng, Feng Rao, Xiumei Wang, Jiang Peng
    更新日期:2018-07-04
  • Preparation of a co-delivery system based on vancomycin/silk scaffold containing silk nanoparticle loaded VEGF
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-03
    Negar Hassani Besheli, Sheyda Damoogh, Bahareh Zafar, Fatemeh Mottaghitalab, Hamidreza Motasadizadeh, Fatemeh Rezaei, Mohammad Ali Shokrgozar, Mehdi Farokhi

    One of the main challenges of using biomaterials for inducing bone regeneration is the bacterial resistance before complete bone repair. Biomaterials with both antibacterial and bone regeneration properties are more promising for bone repair. In the present study, vascular endothelial growth factor (VEGF) was loaded on silk fibroin nanoparticles (SFNPs) and then embedded in silk scaffold containing vancomycin to form a dual drug release system. The chemical and physical properties of the fabricated structure were confirmed by FTIR, SEM, and zeta potential analysis. The size of spherical SFNPs was ~92 nm. The release kinetics of vancomycin and VEGF showed that ~99.56% of vancomycin and ~14% of VEGF were released during 21 and 28 days, respectively. The bioactivity of VEGF was ~75%. Disk diffusion test confirmed the ability of this drug delivery system against methicillin-resistant Staphylococcus aureus (MRSA). Moreover, the expression of the endothelial markers (FLK-1, vWF, and VE-cadherin), alkaline phosphatase and matrix mineral production were higher in VEGF loaded groups. Taken together, the results indicated that the fabricated co-delivery system was able to simultaneously deliver antibiotic and angiogenic factor which can be considered as a potential candidate for the treatment of contaminated bone injuries.

    更新日期:2018-07-04
  • Porous Three-Dimensional Silk Fibroin Scaffolds for Tracheal Epithelial Regeneration in Vitro and in Vivo
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-07-02
    Zhongchun Chen, Nongping Zhong, Jianchuan Wen, Minghui Jia, Yongwei Guo, Zhengzhong Shao, Xia Zhao
    更新日期:2018-07-02
  • Collagen-Mimetic Proteins with Tunable Integrin Binding Sites for Vascular Graft Coatings
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-29
    Juan Felipe Diaz Quiroz, Patricia Diaz Rodriguez, Josh D. Erndt-Marino, Viviana Guiza, Bailey Balouch, Tyler Graf, William M. Reichert, Brooke Russell, Magnus Höök, Mariah S. Hahn
    更新日期:2018-06-30
  • Titanium Surfaces Functionalized with siMIR31HG Promote Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-29
    Yiping Huang, Yunfei Zheng, Yongxiang Xu, Xiaobei Li, Yan Zheng, Lingfei Jia, Weiran Li
    更新日期:2018-06-30
  • Atomistic Modeling of F-Actin Mechanical Responses and Determination of Mechanical Properties
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-29
    Si Li, Jin Zhang, Chengyuan Wang, Perumal Nithiarasu
    更新日期:2018-06-30
  • Biomedical Applications of Graphene Nanomaterials and Beyond
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-29
    Krishanu Ghosal, Kishor Sarkar

    Graphene nanomaterials have been considered as a novel class of nanomaterials that showed exceptional structural, optical, thermal, electrical and mechanical properties. As a consequence it has been extensively studied in various fields including electronics, energy, catalysis, sensing and biomedical fields. In the previous couple of years a significant number of studies have done on graphene based nanomaterials, where it is utilized in a wide range of bioapplications which includes; delivery of small molecule drugs/genes, biosensing, tissue engineering, bioimaging and photothermal and photodynamic therapies due to its excellent aqueous processability, surface functionalizability, outstanding electrical and mechanical properties, tunable fluorescence properties and surface enhanced Raman scattering (SERS).Therefore, it is necessary to get detailed knowledge about it. In this review we will highlight the various synthesis procedures of graphene family nanomaterials including graphene oxide (GO), reduced graphene oxide (rGO) and graphene quantum dots (GQDs) as well as their biomedical applications. We will also highlight the biocompatibity of graphene nanomaterials as well as its possible risk factors for bioapplications. In conclusion we will outline the future prospective and current challenges of graphene nanomaterials for clinical applications.

    更新日期:2018-06-30
  • Dual Stimuli-Responsive Nanoparticle-Incorporated Hydrogels as an Oral Insulin Carrier for Intestine-Targeted Delivery and Enhanced Paracellular Permeation
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-29
    Liang Liu, Ying Zhang, Shuangjiang Yu, Zhiming Yang, Chaoliang He, Xuesi Chen

    For enhanced oral insulin delivery, a strategy of acid-resistant and enteric hydrogels encapsulating insulin-loaded nanoparticles was developed. The nanoparticles were prepared by the formation of an anionic insulin/heparin sodium (Ins/HS) aggregate, followed by coating of chitosan (CS) on the surface. The nanoparticles, tagged as CS/Ins/HS NPs, exhibited excellent mucosa affinity, effective protease inhibition and marked paracellular permeation enhancement. Moreover, to improve the acid-stability of CS/Ins/HS NPs and impart the capacity of intestine-targeted delivery, a pH- and amylase-responsive hydrogel was synthesized via free radical copolymerization, using methacrylic acid as the monomer and acrylate-grafted-carboxymethyl starch as the crosslinker. The resulting hydrogel exhibited sharp pH-sensitivity in gastrointestinal tract and rapid enteric behavior under intestinal amylase. The additional protection for insulin in artificial gastric fluid was confirmed by packaging CS/Ins/HS NPs into the hydrogel. The obtained nanoparticle-incorporated hydrogel was named as NPs@Gel-2. The release of insulin from NPs@Gel-2 was evidently accelerated in artificial intestinal fluid containing α-amylase. Furthermore, the hypoglycemic effects were evaluated with type-1 diabetic rats. Compared to subcutaneous injection of insulin solution, the relative pharmacological availability (rPA) for oral intake of NPs@Gel-2 (30 IU/kg) was determined to be 8.6%, along with rPA of 4.6% for oral administration of unpackaged CS/Ins/HS NPs (30 IU/kg). Finally, the two-week therapeutic outcomes in diabetic rats were displayed after twice-daily treatments by oral intake of NPs@Gel-2, showing the relief of diabetic symptoms and suppression of weight loss in the rats. Therefore, this dual stimuli-responsive nanoparticle-incorporated hydrogel system could be a promising platform for oral insulin delivery.

    更新日期:2018-06-30
  • Manipulable permeability of nanogel encapsulation on cells exerts protective effect against TNF-α-induced apoptosis
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-29
    Wenyan Li, Guohui Zhang, Teng Guan, Xiaosha Zhang, Ali Khosrozadeh, Malcolm Xing, Jiming Kong

    Cell encapsulation using microgel and nanogel, as a strategy of cell surface engineering, can mimic the niches of cells and organoids. The established niche which seasons cells and tissues for the controllable development underlies the superiority of encapsulation on cells. Encapsulation by layer-by-layer nanogel coating is a bottom-up simulation of extracellular matrices via nano or micro packaging of cells in a multi-scale way. We report the nanogel encapsulation on individual neuronal cell for a basic study and application of permeability tuning to regulate cells’ apoptosis. Gelatin and hyaluronic acid (HA) are applied for encapsulating PC12 cells. The permeability of encapsulation on cells can be managed by adjusting different parameters such as material concentration, layer thickness and environmental pH. Eventually, permeability of tumor necrosis factor-α (TNF-α) is controlled by tuning encapsulating parameters for blocking the interaction with TNF-receptor 1, so that cell apoptosis is inhibited. In short, nanogel encapsulation exhibits controllable permeability to different molecules and exerts screen effect on TNF-α for protection. This technique holds great potential in basic biological research and translational research, for example, the protection of transplanted cells against apoptotic factors in target areas.

    更新日期:2018-06-30
  • Erythrocyte-Derived Optical Nano-Probes Doped with ICG-Bound Albumin: Material Characteristics and Evaluation for Cancer Cell Imaging
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-28
    Jenny T Mac, Raviraj Vankayala, Dipti Patel, Sabrina Wueste, Bahman Anvari

    Nano-sized structures activated by near infrared (NIR) photo-excitation can provide an optical platform for image-guided removal of small tumor nodules. We have engineered nanoparticles derived from erythrocytes that can be doped with NIR fluorophore, indocyanine green (ICG). We refer to these constructs as NIR erythrocyte-derived transducers (NETs). The objective of this study was to determine if ICG bound albumin (IbA), as the doping material, could enhance the fluorescence emission of NETs, and evaluate the capability of these nano-probes in imaging cancer cells. Erythrocytes were isolated from bovine whole blood and depleted of hemoglobin to form erythrocyte ghosts (EGs). EGs were then extruded through nano-sized porous membranes in the presence of 10-100 µm ICG or ICG:albumin (1:1 molar ratio) to form ICG- or IbA-doped NETs. The resulting nano-sized constructs were characterized for their diameters, zeta potentials, absorption, and fluorescence emission spectra. We used fluorescence microscopic imaging to evaluate the capability of the constructs in imaging SKOV3 ovarian cancer cells. Based on dynamic light scattering measurements, ICG- and IbA-doped NETs had similar diameter distributions (Z-average diameter of 236 and 238 nm, respectively) in phosphate buffer saline supplemented with 10% fetal bovine serum, which remained nearly constant over the course of two hours at 37°C. Despite a much lower loading efficiency of IbA (≈ 0.7 – 8%) as compared to ICG (10 – 45%), the integrated normalized fluorescence emission of IbA-NETs was two to sixfold higher than ICG-doped NETs. IbA-NETs also demonstrated an enhanced capability in fluorescence imaging of SKOV3 ovarian cancer cells, and can serve as potentially effective nano-probes for fluorescence imaging of cancerous cells.

    更新日期:2018-06-30
  • Engineering Microvascular Networks in LED Light-Cured Cell-Laden Hydrogels
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-26
    Nelson Monteiro, Wenting He, Cristiane Miranda Franca, Avathamsa Athirasala, Luiz E. Bertassoni
    更新日期:2018-06-27
  • Microneedles Integrated with ZnO Quantum-Dot-Capped Mesoporous Bioactive Glasses for Glucose-Mediated Insulin Delivery
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-26
    Bin Xu, Qinying Cao, Yang Zhang, Weijiang Yu, Jiangying Zhu, Depeng Liu, Guohua Jiang
    更新日期:2018-06-27
  • Toxicity of Nanomaterials: Exposure, Pathways, Assessment, and Recent Advances
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-25
    Priyanka Ganguly, Ailish Breen, Suresh C. Pillai
    更新日期:2018-06-27
  • Hemopressin-Based pH-Sensitive Hydrogel: A Potential Bioactive Platform for Drug Delivery
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-25
    Huy Minh Dao, Jun Chen, Bernabe S. Tucker, Vinoy Thomas, Ho-Wook Jun, Xing-Cong Li, Seongbong Jo
    更新日期:2018-06-27
  • Effect of ice nucleation and cryoprotectants during high subzero preservation in endothelialized microchannels
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-26
    Shannon N. Tessier, Lindong Weng, Will D. Moyo, Sam H. Au, Keith H. K. Wong, Cindy Angpraseuth, Amy E. Stoddard, Chenyue Lu, Linda T. Nieman, Rebecca Sandlin, Korkut Uygun, Shannon L Stott, Mehmet Toner

    Cryopreservation is of significance in areas including tissue engineering, regenerative medicine and organ transplantation. We investigated endothelial cell attachment and membrane integrity in a microvasculature model at high subzero temperatures in the presence of extracellular ice. The results show that in the presence of heterogeneous extracellular ice formation induced by ice nucleating bacteria, endothelial cells showed improved attachment at temperature minimums of -6°C. However, as temperatures decreased below -6°C, endothelial cells required additional cryoprotectants. The glucose analog, 3-O-methyl-d-glucose (3-OMG), rescued cell attachment optimally at 100 mM (cells/lane was 34, as compared to 36 for controls), while 2% and 5% polyethylene glycol (PEG) were equally effective at -10°C (88 and 86.4% intact membranes). Finally, engineered capillaries were stored for 72 hours at -10°C in a preservation solution consisting of the University of Wisconsin (UW) solution, Snomax, 3-OMG, PEG, glycerol, and trehalose whereby cell attachment was not significantly different from unfrozen controls, although membrane integrity was compromised. These findings enrich our knowledge about the direct impact of extracellular ice on endothelial cells. Specifically, we show that by controlling the ice nucleation temperature and uniformity we can preserve cell attachment and membrane integrity. Further, we demonstrate the strength of leveraging endothelialized microchannels to fuel discoveries in cryopreservation of thick tissues and solid organs.

    更新日期:2018-06-27
  • Biodegradable, Biomimetic Elastomeric, Photoluminescent and Broad-spectrum Antibacterial Polycitrate-Polypeptide-based Membrane towards Multifunctional Biomedical Implants
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-25
    Feng Li, Yajuan Su, Guofu Pi, Peter X Ma, Bo Lei

    Present biomedical membranes in guiding tissue regeneration applications are almost non-biodegradable or deficient in functionality. The development of biodegradable biomaterials with multifunctional properties including biomimetic elastomeric behavior, self-anti-infection, non-invasive monitoring and good biocompatibility, has attracted much attention. Here, we report a biodegradable and biocompatible polycitrate-(ε-polypeptide)-based (PCE) biomedical elastomers membrane with intrinsical broad-spectrum antibacterial activity and photoluminescent capacity for multifunctional guiding tissue regenerative applications. PCE showed highly elastomeric mechanical behavior (~300% elongation and ~100% recovery) and biomimetic mechanical properties against several native tissues. PCE film also possessed highly efficient broad-spectrum antibacterial activity in vitro and in vivo. The inherent photoluminescent properties of PCE film endowed their real-time non-invasive monitoring capacity in vivo. Owe to the biocompatible structure (polycitrate and natural polypeptide), PCE film demonstrated significantly high cytocompatibility and hemocompatibility in vitro and low inflammatory response in vivo. Our study may provide a new strategy to design next generation multifunctional biodegradable biomedical implants membrane for smart guiding tissue regenerative medicine applications.

    更新日期:2018-06-27
  • Increased Cardiomyocyte Alignment and Intracellular Calcium Transients Using Micropatterned and Drug-Releasing Poly(Glycerol Sebacate) Elastomers
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-21
    Chenghao Zhu, Andrew E. Rodda, Vinh X. Truong, Yue Shi, Kun Zhou, John M. Haynes, Bing Wang, Wayne D. Cook, John S. Forsythe
    更新日期:2018-06-22
  • Chemical Treatment Method for Obtaining Clean and Intact Pollen Shells of Different Species
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-21
    Pedro Gonzalez-Cruz, Md Jasim Uddin, Shashwati U. Atwe, Noureddine Abidi, Harvinder Singh Gill
    更新日期:2018-06-22
  • Microspheres of Carboxymethyl Chitosan, Sodium Alginate, and Collagen as a Hemostatic Agent in Vivo
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-20
    Jia Jin, Zhixiao Ji, Ming Xu, Chenyu Liu, Xiaoqing Ye, Weiyao Zhang, Si Li, Dan Wang, Wenping Zhang, Jianqing Chen, Fei Ye, Zhengbing Lv
    更新日期:2018-06-22
  • An Injectable Decellularized Matrix That Improves Mesenchymal Stem Cell Engraftment for Therapeutic Angiogenesis
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-20
    Gun-Jae Jeong, Seuk Young Song, Mikyung Kang, Seokhyeong Go, Hee Su Sohn, Byung-Soo Kim
    更新日期:2018-06-22
  • Impact of Pore Size and Surface Chemistry of Porous Silicon Particles and Structure of Phospholipids on Their Interactions
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-19
    Dongfei Liu, Katriina Lipponen, Peng Quan, Xiaocao Wan, Hongbo Zhang, Ermei Mäkilä, Jarno Salonen, Risto Kostiainen, Jouni Hirvonen, Tapio Kotiaho, Hélder A. Santos
    更新日期:2018-06-20
  • Advanced Cell and Tissue Biomanufacturing
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-19
    Kaiming Ye, David L. Kaplan, Gang Bao, Christopher Bettinger, Gabor Forgacs, Cheng Dong, Ali Khademhosseini, Yonggang Ke, Kam Leong, Athanassios Sambanis, Wei Sun, Peng Yin
    更新日期:2018-06-20
  • Robust Thin Film Surface with a Selective Antibacterial Property Enabled via a Cross-Linked Ionic Polymer Coating for Infection-Resistant Medical Applications
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-15
    Goro Choi, Gu Min Jeong, Myung Seok Oh, Munkyu Joo, Sung Gap Im, Ki Jun Jeong, Eunjung Lee
    更新日期:2018-06-15
  • Total Eradication of Bacterial Infection in Root Canal Treatment: An Electrochemical Approach
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-14
    Abhijith Segu, Divya Bijukumar, Tina Trinh, Manila Nuchhe Pradhan, Qian Xie, Sukotjo Cortino, Mathew T. Mathew
    更新日期:2018-06-15
  • 更新日期:2018-06-15
  • 更新日期:2018-06-13
  • Multifunctional Hybrid Liposome as a Theranostic Platform for Magnetic Resonance Imaging Guided Photothermal Therapy
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-11
    Chunyang Zhang, Dan Wu, Liejing Lu, Xiaohui Duan, Jie Liu, Xiaoyan Xie, Xintao Shuai, Jun Shen, Zhong Cao
    更新日期:2018-06-12
  • 更新日期:2018-06-08
  • Recent Advances in Biomaterials Science and Engineering Research in India: A Minireview
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-07
    Sunita P. Victor, Shivaram Selvam, Chandra P. Sharma
    更新日期:2018-06-07
  • Mussel-Inspired Peptide Coatings on Titanium Implant to Improve Osseointegration in Osteoporotic Condition
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-07
    Huan Zhao, Yingkang Huang, Wen Zhang, Qianping Guo, Wenguo Cui, Zhiyong Sun, David Eglin, Lei Liu, Guoqing Pan, Qin Shi
    更新日期:2018-06-07
  • Processing and Properties of Chitosan Inks for 3D Printing of Hydrogel Microstructures
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-07
    Qinghua Wu, Daniel Therriault, Marie-Claude Heuzey
    更新日期:2018-06-07
  • A Thermoresponsive Citrate-Based Graphene Oxide Scaffold Enhances Bone Regeneration from BMP9-Stimulated Adipose-Derived Mesenchymal Stem Cells (MSCs)
    ACS Biomater. Sci. Eng. (IF 4.432) Pub Date : 2018-06-07
    Chen Zhao, Zongyue Zeng, Nader Taheri Qazvini, Xinyi Yu, Ruyi Zhang, Shujuan Yan, Yi Shu, Yunxiao Zhu, Chongwen Duan, Elliot Bishop, Jiayan Lei, Wenwen Zhang, Chao Yang, Ke Wu, Ying Wu, Liping An, Shifeng Huang, Xiaojuan Ji, Cheng Gong, Chengfu Yuan, Linghuan Zhang, Wei Liu, Bo Huang, Yixiao Feng, Bo Zhang, Zhengyu Dai, Yi Shen, Xi Wang, Wenping Luo, Leonardo Oliveira, Aravind Athiviraham, Michael J. Lee, Jennifer M. Wolf, Guillermo A Ameer, Russell R Reid, Tong-Chuan He, Wei Huang

    Effective bone tissue engineering is important to overcome the unmet clinical challenges as more than1.6 million bone grafts are done annually in the United States. Successful bone tissue engineering needs minimally three critical constituents, osteoprogenitor cells, osteogenic factors, and osteoinductive/osteoconductive scaffolds. Osteogenic progenitors are derived from multipotent mesenchymal stem cells (MSCs), which can be prepared from numerous tissue sources including adipose tissue. We previously showed that BMP9 is the most osteogenic BMP and induces robust bone formation of immortalized mouse adipose-derived MSCs (iMADs) entrapped in a citrate-based thermoresponsive hydrogel referred to as PPCNg. As graphene and its derivatives emerge as promising biomaterials, here we develop a novel thermosensitive and injectable hybrid material by combining graphene oxide (GO) with PPCNg (designated as GO-P) and characterize its ability to promote bone formation. We demonstrate that the thermoresponsive behavior of the hybrid material is maintained while effectively supporting MSC survival and proliferation. Furthermore, GO-P induces early bone-forming marker alkaline phosphatase (ALP) and potentiates BMP9-induced expression of osteogenic regulators and bone markers, as well as angiogenic factor VEGF in MSCs. In vivo studies show BMP9-transduced MSCs entrapped in the GO-P scaffold form well-mineralized and highly-vascularized trabecular bone. Thus, these results indicate that GO-P hybrid material may function as a new biocompatible, injectable scaffold with osteoinductive and osteoconductive activities for bone regeneration.

    更新日期:2018-06-07
Some contents have been Reproduced with permission of the American Chemical Society.
Some contents have been Reproduced by permission of The Royal Society of Chemistry.
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