This review focuses on the biogenesis and composition of the eukaryotic DNA replication fork, with an emphasis on the enzymes that synthesize DNA and repair discontinuities on the lagging strand of the replication fork. Physical and genetic methodologies aimed at understanding these processes are discussed. The preponderance of evidence supports a model in which DNA polymerase ε (Pol ε) carries out the bulk of leading strand DNA synthesis at an undisturbed replication fork. DNA polymerases α and δ carry out the initiation of Okazaki fragment synthesis and its elongation and maturation, respectively. This review also discusses alternative proposals, including cellular processes during which alternative forks may be utilized, and new biochemical studies with purified proteins that are aimed at reconstituting leading and lagging strand DNA synthesis separately and as an integrated replication fork.

中文翻译：

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真核DNA复制叉。

这篇综述集中在真核DNA复制叉的生物发生和组成上，着重于合成DNA和修复复制叉的落后链上的不连续性的酶。讨论了旨在理解这些过程的物理和遗传方法。大量证据支持这样一种模型，其中DNA聚合酶ε（Polε）在不受干扰的复制叉处进行大量的前导DNA合成。DNA聚合酶α和δ分别启动Okazaki片段的合成以及其延伸和成熟。这篇评论还讨论了替代方案，包括在蜂窝过程中可以利用替代叉的过程，