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Whole Grain Proso Millet (Panicum miliaceum L.) Attenuates Hyperglycemia in Type 2 Diabetic Mice: Involvement of miRNA Profile
Journal of Agricultural and Food Chemistry ( IF 6.1 ) Pub Date : 2023-06-09 , DOI: 10.1021/acs.jafc.2c08184
Xu Deng 1 , Jiayi Liang 1 , Lehui Wang 1 , Li Niu 1 , Jin Xiao 1 , Qianqian Guo 1 , Xuebo Liu 1 , Chunxia Xiao 1
Affiliation  

This work aimed to investigate the hypoglycemic effects and underlying mechanism of whole grain proso millet (Panicum miliaceum L.; WPM) on type 2 diabetes mellitus (T2DM). The results showed that WPM supplementation significantly reduced fasting blood glucose (FBG) and serum lipid levels in T2DM mice induced by a high-fat diet (HFD) combined with streptozotocin (STZ), with improved glucose tolerance, liver and kidney injury, and insulin resistance. In addition, WPM significantly inhibited the expression of gluconeogenesis-related genes G6pase, Pepck, Foxo1, and Pgc-1α. Further study by miRNA high-throughput sequencing revealed that WPM supplementation mainly altered the liver miRNA expression profile of T2DM mice by increasing the expression of miR-144–3p_R-1 and miR-423–5p, reducing the expression of miR-22–5p_R-1 and miR-30a-3p. GO and KEGG analyses showed that the target genes of these miRNAs were mainly enriched in the PI3K/AKT signaling pathway. WPM supplementation significantly increased the level of PI3K, p-AKT, and GSK3β in the liver of T2DM mice. Taken together, WPM exerts antidiabetic effects by improving the miRNA profile and activating the PI3K/AKT signaling pathway to inhibit gluconeogenesis. This study implies that PM can act as a dietary supplement to attenuate T2DM.

中文翻译:

全麦 Proso 小米 (Panicum miliaceum L.) 减轻 2 型糖尿病小鼠的高血糖:miRNA 谱的参与

本研究旨在探讨全麦小米(Panicum miliaceum L.;WPM)对 2 型糖尿病(T2DM)的降血糖作用及其潜在机制。结果表明,补充WPM可显着降低高脂饮食(HFD)联合链脲佐菌素(STZ)诱导的T2DM小鼠的空腹血糖(FBG)和血脂水平,并改善糖耐量、肝肾损伤和胰岛素水平反抗。此外,WPM 显着抑制糖异生相关基因G6pasePepckFoxo1Pgc -1α的表达。miRNA高通量测序的进一步研究表明,补充WPM主要通过增加miR-144–3p_R-1和miR-423–5p的表达,减少miR-22–5p_R的表达来改变T2DM小鼠的肝脏miRNA表达谱。 -1 和 miR-30a-3p。GO和KEGG分析表明这些miRNA的靶基因主要富集于PI3K/AKT信号通路。补充 WPM 显着增加 T2DM 小鼠肝脏中 PI3K、p-AKT 和 GSK3β 的水平。综上所述,WPM 通过改善 miRNA 谱并激活 PI3K/AKT 信号通路抑制糖异生来发挥抗糖尿病作用。这项研究表明,PM 可以作为膳食补充剂来减轻 T2DM。
更新日期:2023-06-09
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