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Correction to “CircSETBP1 Acts as a MiR-149-5p Sponge to Promote Intramuscular Fat Deposition by Regulating CRTCs”
Journal of Agricultural and Food Chemistry ( IF 6.1 ) Pub Date : 2023-06-08 , DOI: 10.1021/acs.jafc.3c03133 Yingke Liu , Yaqing Dou , Kunlong Qi , Chenlei Li , Chenglei Song , Xinjian Li , Xiuling Li , Ruimin Qiao , Kejun Wang , Xuelei Han
Journal of Agricultural and Food Chemistry ( IF 6.1 ) Pub Date : 2023-06-08 , DOI: 10.1021/acs.jafc.3c03133 Yingke Liu , Yaqing Dou , Kunlong Qi , Chenlei Li , Chenglei Song , Xinjian Li , Xiuling Li , Ruimin Qiao , Kejun Wang , Xuelei Han
Figure 1A has been modified. The corrected figure is given below. Figure 1. Identification of induced differentiation of porcine IM preadipocytes. (A–C) Cell growth on the first, fourth, and sixth day. (D, E) Differentiation was induced on the sixth and eighth day. (F) Oil red O staining of porcine IM preadipocytes differentiation on the eighth day. (G) Identification of induced differentiation marker genes. The data are expressed as the average ± SEM (n = 3). Significance was determined via t test analysis (* means P < 0.05 and ** means P < 0.01). In Figure 6A, the sequence of miR-149-5p was modified. The corrected figure is given below. Figure 6. CircSETBP1 targeting miR-149-5p was verified. (A) Structure of the circSETBP1 dual-luciferase reporter vector of porcine IM preadipocytes. (B) Dual-luciferase assay detected porcine circSETBP1 in 293T cells. (C) Expression of miR-149-5p after the overexpression of circSEBP1. In Figure 10A,B, the sequence of miR-149-5p was modified. The corrected figure is given below. The authors declare that these corrections do not change the results or conclusions of this paper. We sincerely apologize for having these errors in the article, and apologize for any inconvenience caused. Figure 10. Validation of the miR-149-5p target gene. (A, B) CRTC1 and CRTC2 3′UTR dual-luciferase reporter vector construction diagram in porcine IM preadipocytes and 3T3-L1 cells. (C) Dual-luciferase assay detected CRTC1 and CRTC2 in the porcine IM preadipocytes. (D) Dual-luciferase assay detected CRTC1 and CRTC2 in the 3T3-L1 cells. (E) Validation of mRNA levels of CRTC1 and CRTC2 in the porcine IM preadipocytes and 3T3-L1 cells. This article has not yet been cited by other publications. Figure 1. Identification of induced differentiation of porcine IM preadipocytes. (A–C) Cell growth on the first, fourth, and sixth day. (D, E) Differentiation was induced on the sixth and eighth day. (F) Oil red O staining of porcine IM preadipocytes differentiation on the eighth day. (G) Identification of induced differentiation marker genes. The data are expressed as the average ± SEM (n = 3). Significance was determined via t test analysis (* means P < 0.05 and ** means P < 0.01). Figure 6. CircSETBP1 targeting miR-149-5p was verified. (A) Structure of the circSETBP1 dual-luciferase reporter vector of porcine IM preadipocytes. (B) Dual-luciferase assay detected porcine circSETBP1 in 293T cells. (C) Expression of miR-149-5p after the overexpression of circSEBP1. Figure 10. Validation of the miR-149-5p target gene. (A, B) CRTC1 and CRTC2 3′UTR dual-luciferase reporter vector construction diagram in porcine IM preadipocytes and 3T3-L1 cells. (C) Dual-luciferase assay detected CRTC1 and CRTC2 in the porcine IM preadipocytes. (D) Dual-luciferase assay detected CRTC1 and CRTC2 in the 3T3-L1 cells. (E) Validation of mRNA levels of CRTC1 and CRTC2 in the porcine IM preadipocytes and 3T3-L1 cells.
中文翻译:
对“CircSETBP1 作为 MiR-149-5p 海绵通过调节 CRTC 促进肌内脂肪沉积”的更正
图 1A 已被修改。修正后的数字如下。图 1. 猪 IM 前脂肪细胞诱导分化的鉴定。(A–C) 第一天、第四天和第六天的细胞生长。(D、E)在第六天和第八天诱导分化。(F)第八天猪肌内前脂肪细胞分化的油红O染色。(G)诱导分化标记基因的鉴定。数据表示为平均值±SEM(n = 3)。通过t检验分析确定显着性(* 表示P < 0.05,** 表示P< 0.01)。在图6A中,miR-149-5p的序列被修改。修正后的数字如下。图 6. CircSETBP1 靶向 miR-149-5p 已得到验证。(A) 猪 IM 前脂肪细胞的 circSETBP1 双荧光素酶报告载体的结构。(B) 双荧光素酶测定在 293T 细胞中检测到猪 circSETBP1。(C) circSEBP1 过表达后 miR-149-5p 的表达。在图10A、B中,miR-149-5p的序列被修饰。修正后的数字如下。 作者声明这些更正不会改变本文的结果或结论。对于文章中的这些错误,我们深表歉意,并对由此造成的任何不便表示歉意。图 10. miR-149-5p 靶基因的验证。(A, B) CRTC1 和 CRTC2 3'UTR 双荧光素酶报告基因载体在猪 IM 前脂肪细胞和 3T3-L1 细胞中的构建图。(C) 双荧光素酶测定检测猪 IM 前脂肪细胞中的 CRTC1 和 CRTC2。(D) 双荧光素酶测定检测到 3T3-L1 细胞中的 CRTC1 和 CRTC2。(E) 猪 IM 前脂肪细胞和 3T3-L1 细胞中 CRTC1 和 CRTC2 mRNA 水平的验证。这篇文章尚未被其他出版物引用。图 1. 猪 IM 前脂肪细胞诱导分化的鉴定。(A–C) 第一天、第四天和第六天的细胞生长。(D,E)在第六天和第八天诱导分化。(F)第八天猪肌内前脂肪细胞分化的油红O染色。(G)诱导分化标记基因的鉴定。数据表示为平均值±SEM(n = 3)。通过t检验分析确定显着性(* 表示P < 0.05,** 表示P< 0.01)。图 6. CircSETBP1 靶向 miR-149-5p 已得到验证。(A) 猪 IM 前脂肪细胞的 circSETBP1 双荧光素酶报告载体的结构。(B) 双荧光素酶测定在 293T 细胞中检测到猪 circSETBP1。(C) circSEBP1 过表达后 miR-149-5p 的表达。图 10. miR-149-5p 靶基因的验证。(A, B) CRTC1 和 CRTC2 3'UTR 双荧光素酶报告基因载体在猪 IM 前脂肪细胞和 3T3-L1 细胞中的构建图。(C) 双荧光素酶测定检测猪 IM 前脂肪细胞中的 CRTC1 和 CRTC2。(D) 双荧光素酶测定检测到 3T3-L1 细胞中的 CRTC1 和 CRTC2。(E) 猪 IM 前脂肪细胞和 3T3-L1 细胞中 CRTC1 和 CRTC2 mRNA 水平的验证。
更新日期:2023-06-08
中文翻译:
对“CircSETBP1 作为 MiR-149-5p 海绵通过调节 CRTC 促进肌内脂肪沉积”的更正
图 1A 已被修改。修正后的数字如下。图 1. 猪 IM 前脂肪细胞诱导分化的鉴定。(A–C) 第一天、第四天和第六天的细胞生长。(D、E)在第六天和第八天诱导分化。(F)第八天猪肌内前脂肪细胞分化的油红O染色。(G)诱导分化标记基因的鉴定。数据表示为平均值±SEM(n = 3)。通过t检验分析确定显着性(* 表示P < 0.05,** 表示P< 0.01)。在图6A中,miR-149-5p的序列被修改。修正后的数字如下。图 6. CircSETBP1 靶向 miR-149-5p 已得到验证。(A) 猪 IM 前脂肪细胞的 circSETBP1 双荧光素酶报告载体的结构。(B) 双荧光素酶测定在 293T 细胞中检测到猪 circSETBP1。(C) circSEBP1 过表达后 miR-149-5p 的表达。在图10A、B中,miR-149-5p的序列被修饰。修正后的数字如下。