Separation and Purification Technology ( IF 8.6 ) Pub Date : 2023-05-27 , DOI: 10.1016/j.seppur.2023.124226 Aye Aye Myint, Sabrinna Wulandari, Jongho Choi, Sang Jun Sim, Jaehoon Kim
Efficient and eco-friendly methods for extracting bioactive molecules are crucial for achieving economically viable microalgae biorefinery. In this study, high-value-added intracellular bioactive compounds were efficiently recovered directly from the red cysts of wet Haematococcus pluvialis. An integrated processes involving liquid dimethyl ether (l-DME) extraction at 45 °C and 2 MPa for 90 min and subsequent dimethyl sulfoxide (DMSO) extraction at 65 °C for 5 min enabled almost complete recovery of astaxanthin (99.6%) and total fatty acids (99.8%) from wet H. pluvialis cyst cells. Notably, this green and sustainable strategy did not involve high-cost and energy-intensive drying, or cell wall-disruption steps. l-DME extraction produced a solvent-free, dry extract with high astaxanthin (43.9 mg g−1 dry extract) and essential fatty acids (ω3, ω6, and ω9; 290.1 mg g−1 dry extract) contents; whereas the astaxanthin and fatty acid contents of the DMSO extract produced from raw H. pluvialis cyst cells (freeze-dried and ball-milled at 200 rpm for 30 min) were 1.5- and 1.7-fold lower, respectively. The dry extract also exhibited 1.6-fold greater antioxidant activity than the DMSO extract, thereby indicating its potential for direct application in nutraceutical, pharmaceutical, and cosmeceutical formulations with high bioactivity and safety.
中文翻译:
基于二甲醚的高产率直接从湿雨生红球藻中回收虾青素和脂肪酸
用于提取生物活性分子的高效且环保的方法对于实现经济上可行的微藻生物精炼至关重要。在这项研究中,高附加值的细胞内生物活性化合物直接从湿雨生红球藻的红色包囊中高效回收。包括在 45 °C 和 2 MPa 条件下进行 90 分钟液体二甲醚 ( l -DME) 提取以及随后在 65 °C 条件下进行 5 分钟二甲亚砜 (DMSO) 提取的综合工艺几乎可以完全回收虾青素 (99.6%) 和总虾青素来自湿H. pluvialis囊肿细胞的脂肪酸 (99.8%) 。值得注意的是,这种绿色和可持续的战略不涉及高成本和能源密集型干燥或细胞壁破坏步骤。升-DME 提取产生了一种无溶剂的干提取物,具有高虾青素(43.9 mg g -1干提取物)和必需脂肪酸(ω3、ω6 和 ω9;290.1 mg g -1干提取物)含量;而从原始雨生雨生囊肿细胞(冷冻干燥并以 200 rpm 球磨 30 分钟)产生的 DMSO 提取物中虾青素和脂肪酸含量分别低 1.5 倍和 1.7 倍。干提取物还表现出比 DMSO 提取物高 1.6 倍的抗氧化活性,从而表明它具有直接应用于具有高生物活性和安全性的保健品、药物和药妆配方的潜力。