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Molecular characterization, immune functions and DNA protective effects of peroxiredoxin-1 gene in Antheraea pernyi Mol. Immunol. (IF 3.6) Pub Date : 2024-04-18 Muhammad Nadeem Abbas, Isma Gul, Zahra Khosravi, Jemirade Ifejola Amarchi, Xiang Ye, Lang Yu, Wu Siyuan, Hongjuan Cui
Peroxiredoxins are antioxidant proteins that detoxify peroxynitrite, hydrogen peroxide, and organic hydroperoxides, impacting various physiological processes such as immune responses, apoptosis, cellular homeostasis, and so on. In the present study, we identified and characterized peroxiredoxin 1 from (thereafter designated as ) that encodes a predicted 195 amino acid residue protein with a 21.8 kDa
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Recruitment or activation of mast cells in the liver aggravates the accumulation of fibrosis in carbon tetrachloride-induced liver injury Mol. Immunol. (IF 3.6) Pub Date : 2024-04-15 Mingkang Zhang, Jinru Yang, Yufan Yuan, Yan Zhou, Yazhi Wang, Ruirui Cui, Yimai Maliu, Fen Xu, Xin’an Wu
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Peritoneal B1 and B2 cells respond differently to LPS and IL-21 stimulation Mol. Immunol. (IF 3.6) Pub Date : 2024-04-13 Dandan Li, Yanfen Ma, Yinsha Miao, Sasa Liu, Yu Bi, Yanhong Ji, Qifei Wu, Can Zhou, Yunfeng Ma
Peritoneal B cells can be divided into B1 cells (CD11bCD19) and B2 cells (CD11bCD19) based on CD11b expression. B1 cells play a crucial role in the innate immune response by producing natural antibodies and cytokines. B2 cells share similar traits with B1 cells, influenced by the peritoneal environment. However, the response of both B1 and B2 cells to the same stimuli in the peritoneum remains uncertain
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Neuroplastin splice variants Np55 and Np65: Who is doing the job in macrophages? Mol. Immunol. (IF 3.6) Pub Date : 2024-04-13 Kristina Langnaese, Nikhil Tiwari, Klaus-Dieter Fischer, Ulrich Thomas, Mark Korthals
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SHIP inhibition mediates select TREM2-induced microglial functions Mol. Immunol. (IF 3.6) Pub Date : 2024-04-12 Gautham S. Ramakrishnan, William L. Berry, Angela Pacherille, William G. Kerr, John D. Chisholm, Chiara Pedicone, Mary Beth Humphrey
Microglia play a pivotal role in the pathology of Alzheimer's Disease (AD), with the Triggering Receptor Expressed on Myeloid cells 2 (TREM2) central to their neuroprotective functions. The R47H variant of TREM2 has emerged as a significant genetic risk factor for AD, leading to a loss-of-function phenotype in mouse AD models. This study elucidates the roles of TREM2 in human microglia-like HMC3 cells
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Ontogeny of myeloperoxidase (MPO) positive cells in flounder (Paralichthys olivaceus) Mol. Immunol. (IF 3.6) Pub Date : 2024-04-10 Qiujie Gan, Heng Chi, Chengcheng Liang, Letao Zhang, Roy Ambli Dalmo, Xiuzhen Sheng, Xiaoqian Tang, Jing Xing, Wenbin Zhan
Neutrophils represent an important asset of innate immunity. Neutrophils express myeloperoxidase (MPO) which is a heme-containing peroxidase involved in microbial killing. In this study, by using real-time quantitative PCR and Western blot analysis, the flounder MPO (MPO) was observed to be highly expressed in the head kidney, followed by spleen, gill, and intestine during ontogeny – during developmental
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Humanization of a mouse anti-human complement C6 monoclonal antibody as a potential therapeutic for certain complement-mediated diseases Mol. Immunol. (IF 3.6) Pub Date : 2024-04-10 Lingjun Zhang, Kathryn Armour, Jin Y. Chen, Agathi Mylona, Maojing Yang, Gregers R. Andersen, Jaroslaw P. Maciejewki, Preeti Bakrania, Feng Lin
The assembly of tissue-damaging membrane attack complexes (MACs; C5b–9) is a major mechanism by which excessive complement activation causes diseases. We previously developed a mouse anti-human C6 monoclonal antibody (mAb) 1C9 that selectively inhibits the assembly of MACs in human and non-human primates. In this project, we found that 1C9 also cross-reacted with rat and guinea pig C6, and determined
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KLF5-mediated pyroptosis of airway epithelial cells leads to airway inflammation in asthmatic mice through the miR-182–5p/TLR4 axis Mol. Immunol. (IF 3.6) Pub Date : 2024-04-09 Zhi Lin, Rong Bao, Yang Niu, Xiaomei Kong
Asthma is viewed as an airway disease and an inflammatory condition. This study aims to reveal the role of Kruppel-like factor 5 (KLF5)-mediated pyroptosis of airway epithelial cells in airway inflammation in asthma. The asthmatic mouse model was established. The mice were infected with the lentivirus containing sh-KLF5, antagomiR-182–5p, and pc-Toll-like receptor 4 (TLR4). Airway hyperresponsiveness
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CD1 molecules: Beyond antigen presentation Mol. Immunol. (IF 3.6) Pub Date : 2024-04-04 Lauren Evans, Patricia Barral
CD1 molecules are well known for their role in binding and presenting lipid antigens to mediate the activation of CD1-restricted T cells. However, much less appreciated is the fact that CD1 molecules can have additional “unconventional” roles which impact the activation and functions of CD1-expressing cells, ultimately controlling tissue homeostasis as well as the progression of inflammatory and infectious
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Systems and Computational Screening identifies SRC and NKIRAS2 as Baseline Correlates of Risk (CoR) for Live Attenuated Oral Typhoid Vaccine (TY21a) associated Protection Mol. Immunol. (IF 3.6) Pub Date : 2024-03-29 Akshayata Naidu, Varin Garg, Deepna Balakrishnan, Vinaya C.R, Vino Sundararajan, Sajitha Lulu S
We investigated the molecular underpinnings of variation in immune responses to the live attenuated typhoid vaccine (Ty21a) by analyzing the baseline immunological profile. We utilized gene expression datasets obtained from the Gene Expression Omnibus (GEO) database (accession number: GSE100665) before and after immunization. We then employed two distinct computational approaches to identify potential
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Hyperactivation of succinate dehydrogenase promotes pyroptosis of macrophage via ROS-induced GSDMD oligomerization in acute liver failure Mol. Immunol. (IF 3.6) Pub Date : 2024-03-28 Jiao Yang, JingWen Liang, Cai Huang, ZaiCheng Wu, YanChang Lei
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Melanoma extracellular vesicles inhibit tumor growth and metastasis by stimulating CD8 T cells Mol. Immunol. (IF 3.6) Pub Date : 2024-03-21 Yuxi Dan, Jing Ma, Yuqing Long, Yao Jiang, Liaoqiong Fang, Jin Bai
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Paeonol interferes with lupus nephritis by regulating M1/M2 polarization of macrophages Mol. Immunol. (IF 3.6) Pub Date : 2024-03-18 Yuzhen Niu, Yingying Jin, Yongxi Hao, Wei Liang, Fan Tang, Ziyi Qin, Tao Liang, Le Shi
Systemic lupus erythematosus (SLE) is a complex autoimmune disease of unknown etiology. It is marked by the production of pathogenic autoantibodies and the deposition of immune complexes. Lupus nephritis (LN) is a prevalent and challenging clinical complications of SLE. Cortex Moutan contains paeonol as its main effective component. In this study, using the animal model of SLE induced by R848, it was
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CXCL1-CXCR2 axis mediates inflammatory response after sciatic nerve injury by regulating macrophage infiltration Mol. Immunol. (IF 3.6) Pub Date : 2024-03-16 Suli Jiang, Wei Li, Meiying Song, Jie Liang, Guixian Liu, Qiaochu Du, Luoyang Wang, Haining Meng, Lei Tang, Yanyan Yang, Bei Zhang
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Isoliquiritigenin reduces experimental autoimmune prostatitis by facilitating Nrf2 activation and suppressing the NLRP3 inflammasome pathway Mol. Immunol. (IF 3.6) Pub Date : 2024-03-16 Rui Feng, Tong Meng, Xiaohu Zhao, Weidong Yu, Haolin Li, Zicheng Wang, Jing Chen, Cheng Yang
Chronic prostatitis and chronic pelvic pain syndrome (CP/CPPS) lead to severe irritation and impaired sperm quality in males. However, current therapeutic options often fail to achieve satisfactory effects. Consequently, the investigation of novel treatment strategies or remedies holds substantial clinical importance. As a flavonoid monomer, isoliquiritigenin (ISL) has been shown to possess anti-inflammatory
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MS4A6D Promotes carrageenan-induced footpad swelling in mice through enhancing macrophages-derived inflammation Mol. Immunol. (IF 3.6) Pub Date : 2024-03-16 Jing Guo, Chenhui Wang, Qun Xiang, Zeqing Feng, Lei Fei, Guoning Guo, Yongjun Shang, Yunfei An, Zhihua Ruan, Yongwen Chen, Xiaoyong Huang
Our previous work has demonstrated that the tetraspan MS4A6D interacts with MHC-II to be a complex that promotes macrophage activation (Mol Immunol. 2023; 160: 121–132), however, the exact role of MS4A6D in controlling macrophage-derived inflammation is still poorly understood. Here, we showed that -deficient () mice manifested a lower level of footpad swelling induced by subcutaneous injection of
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Melatonin improves salivary gland damage and hypofunction in pSS by inhibiting IL-6/STAT3 signaling through its receptor-dependent manner Mol. Immunol. (IF 3.6) Pub Date : 2024-03-10 Yi Liu, Fang Wang, Bo Cheng, Gang Zhou
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Recent advances in potential targets for myocardial ischemia reperfusion injury: Role of macrophages Mol. Immunol. (IF 3.6) Pub Date : 2024-03-05 Qigang Zhuang, Mingyue Li, Desheng Hu, Junyi Li
Myocardial ischemia-reperfusion injury (MIRI) is a complex process that occurs when blood flow is restored after myocardium infarction (MI) with exacerbated tissue damage. Macrophages, essential cell type of the immune response, play an important role in MIRI. Macrophage subpopulations, namely M1 and M2, are distinguished by distinct phenotypes and functions. In MIRI, macrophages infiltrate in infarcted
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Exploring the molecular and immune-landscape of lung cancer associated with cystic airspaces Mol. Immunol. (IF 3.6) Pub Date : 2024-03-01 Xiang Zheng, Li Qiu, Ying Huang, Ran Cheng, Sihe Huang, Ke Xu, Weipeng Cai, Yu Deng, Wei Wang, Xi Zhong, Fei Cui, Zhexue Hao, Jun Liu
To explore the molecular biological characteristics of lung cancer associated with cystic airspaces (LCCA) and its potential roles on prognosis. A total of 165 LCCAs and 201 non-LCCAs were enrolled in this study. Bulk RNA sequencing was implemented in eight LCCAs and nine non-LCCAs to explore the differentially expressed genes. TCGA data were used to analyze LCCA-specific genes that associated with
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Hydrogen ameliorates endotoxin-induced acute lung injury through AMPK-mediated bidirectional regulation of Caspase3 Mol. Immunol. (IF 3.6) Pub Date : 2024-02-29 Qian Li, Min Shi, Yang Ang, Pan Yu, Bing Wan, Bin Lin, Wei Chen, Zichuan Yue, Yadan Shi, Faqi Liu, Hao Wang, Manlin Duan, Yun Long, Hongguang Bao
Septic lung injury is characterized by uncontrollable inflammatory infiltrations and acute onset bilateral hypoxemia. Evidence has emerged of the beneficial effect of hydrogen in acute lung injury (ALI), but the underlying mechanism is unclear. In this research, the recovery action of hydrogen on lipopolysaccharide (LPS)-induced ALI in mice and A549 cells was investigated. The 7-day survival rate and
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Silencing of forkhead box C1 reduces nasal epithelial barrier damage in mice with allergic rhinitis via epigenetically upregulating secreted frizzled-related protein 5 Mol. Immunol. (IF 3.6) Pub Date : 2024-02-29 Zhaohui Shi, Tianfeng Zhao, Dingbo Li, Chong Wang, Yanjie Luo, Yangshan Zheng
Allergic rhinitis (AR) is caused by immunoglobulin E (IgE)-mediated reactions to inhaled allergens, which leads to mucosal inflammation and barrier dysfunction. The transcription factor forkhead box C1 (FOXC1) has been identified to be associated with allergic inflammation. This study sought to uncover the role of FOXC1 in AR. A murine model of AR was induced by repeated intranasal ovalbumin (OVA)
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The NLRP3 molecule is responsible for mediating the pyroptosis of intestinal mucosa cells and plays a crucial role in salmonellosis enteritis in chicks Mol. Immunol. (IF 3.6) Pub Date : 2024-02-29 Zhe Liu, Juan Du, Yanhong Wang, Haoyu Song, Ligong Lu, Rui Wu, Chenghao Jin
Salmonella enteritis in poultry can result in reduced immune function, decreased growth rate, and increased mortality. Many farm salmonella strains have developed severe drug resistance and are less susceptible to multiple antibiotics. In the post-antibiotic era, it is of great significance to identify the mechanism of salmonella-induced enteritis in chicks to protect their health and ensure food safety
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Human beta defensin 3 knockdown inhibits the proliferation and migration of airway smooth muscle cells through regulating the PI3K/AKT signaling pathway Mol. Immunol. (IF 3.6) Pub Date : 2024-02-28 Guiying Chen, Yuling Zheng, Nan Wu, Xia Yang, Shuqiang Qu
Asthma, a common pediatric pulmonary disease, significantly affects children’s healthy development. This study aimed to investigate the functions of human β defensin-3 (HBD-3) in asthma progression. For this purpose, blood samples from asthmatic and healthy children were collected. Moreover, the airway smooth muscle cells (ASMCs) were treated with platelet-derived growth factor BB (PDGF-BB) to develop
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Predicting the risk of interstitial lung disease in patients with primary Sjögren's syndrome: Novel nomogram and elevated Th2 cells Mol. Immunol. (IF 3.6) Pub Date : 2024-02-20 Yanlin Wang, Yuhan Jia, Yan Qin, Min Feng, Zhaojun Liang, Xiangcong Zhao, Chong Gao, Hui Guo, Jing Luo
Interstitial lung disease (ILD) is one of the most common pulmonary complications in patients with primary Sjögren’s syndrome (pSS). This study was performed to identify immunological risk factors of pSS-associated ILD (pSS-ILD) and to further establish and evaluate of nomograms predicting the risk of ILD in patients with pSS. A total of 622 patients with pSS (117 with ILD and 505 without lung involvement)
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SAMHD1 dysfunction induces IL-34 expression via NF-κB p65 in neuronal SH-SY5Y cells Mol. Immunol. (IF 3.6) Pub Date : 2024-02-17 Ling Zhang, Wenjing Wang, Ting Chen, Jiuhao Cui, Xin Li, Anran Liu, Rumeng Liu, Liwei Fang, Junhong Jiang, Li Yang, De Wu, Songcheng Ying
Dysfunctional mutations in SAMHD1 cause Aicardi-Goutières Syndrome, an autoinflammatory encephalopathy with elevated interferon-α levels in the cerebrospinal fluid. Whether loss of function mutations in SAMHD1 trigger the expression of other cytokines apart from type I interferons in Aicardi-Goutières Syndrome is largely unclear. This study aimed to explore whether SAMHD1 dysfunction regulated the
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Shared genetic architecture between hypothyroidism and rheumatoid arthritis: A large-scale cross-trait analysis Mol. Immunol. (IF 3.6) Pub Date : 2024-02-17 Ruiyan Liu, Xin Shang, Yu Fu, Ying Wang, Ping Wang, Shuxun Yan
In recent years, mounting evidence has indicated a co-morbid relationship between hypothyroidism and rheumatoid arthritis (RA), however, the shared genetic factors underlying this association remain unclear. This study aims to investigate the common genetic architecture between hypothyroidism and RA. Genome-wide association study (GWAS) summary statistics from recently published studies were utilized
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Efficacy of GalNAc C3 siRNAs in factor H-deficient mice with C3 glomerulopathy Mol. Immunol. (IF 3.6) Pub Date : 2024-02-17 Cristina Zanchi, Monica Locatelli, Domenico Cerullo, Verena Aumiller, Daniela Corna, Daniela Rottoli, Steffen Schubert, Marina Noris, Susanna Tomasoni, Giuseppe Remuzzi, Carlamaria Zoja, Ariela Benigni
Complement alternative pathway (AP) dysregulation drives C3 glomerulopathy (C3G), a rare renal disorder characterized by glomerular C3 deposition and glomerular damage, for which no effective treatments are available. Blockade of complement C3 is emerging as a viable therapeutic option. In an earlier study we showed that SLN500, a small interfering RNA targeting liver C3 synthesis, was able to limit
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Empagliflozin attenuates inflammation levels in autoimmune myocarditis through the STAT3 pathway and macrophage phenotype transformation Mol. Immunol. (IF 3.6) Pub Date : 2024-02-14 Wenxin Sheng, Jianshu Yu, Hao Zhang, Jingbo Zhang
To investigate the anti-inflammatory actions and molecular mechanisms of the sodium/glucose cotransporter 2 (SGLT-2) inhibitor empagliflozin on autoimmune myocarditis. The experimental autoimmune myocarditis (EAM) mouse model was constructed using peptides, and the therapeutic effects of empagliflozin on cardiac inflammation and fibrosis were observed using hematoxylin and eosin (HE), Sirius red staining
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SARS-CoV-2 spike protein receptor binding domain promotes IL-6 and IL-8 release via ATP/P2Y2 and ERK1/2 signaling pathways in human bronchial epithelia Mol. Immunol. (IF 3.6) Pub Date : 2024-02-14 Rui-Gang Zhang, Xing-Jian Liu, Yu-Ling Guo, Chun-Ling Chen
The spike protein of SARS-CoV-2 as well as its receptor binding domain (RBD) has been demonstrated to be capable of activating the release of pro-inflammatory mediators in endothelial cells and immune cells such as monocytes. However, the effects of spike protein or its RBD on airway epithelial cells and mechanisms underlying these effects have not been adequately characterized. Here, we show that
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The role of transforming growth factor beta in myopia development Mol. Immunol. (IF 3.6) Pub Date : 2024-02-10 Hsiangyu Ku, Jamie Jiin-Yi Chen, Wei Chen, Peng-Tai Tien, Hui-Ju Lin, Lei Wan, Gezhi Xu
Myopia is widely recognized as an epidemic. Studies have found a link between Transforming Growth Factor-beta (TGF-β) and myopia, but the specific molecular mechanisms are not fully understood. In this study, a monocular model in tree shrews () was established to verify the molecular mechanism of TGF-β in myopia. The results indicated that there were significant changes in TGF-βs during the treatment
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Anti-inflammation of LZTFL1 knockdown in OVA-induced asthmatic mice: Through ERK/GATA3 signaling pathway Mol. Immunol. (IF 3.6) Pub Date : 2024-02-03 Guihua Song, Mengmeng Sun, Yan Zhang, Bingxue Zhang, Minghao Peng, Beibei Bao
Asthma is a common chronic respiratory disease characterized by Th2-type inflammation in the airways. Leucine zip transcription factor-like 1 (LZTFL1) has been implicated in the regulation of Th2-related factors. The knockdown of LZTFL1 resulted in decreased levels of IL-4, IL-5, and IL-13. We hypothesize that LZTFL1 may have an effect on asthma. We established an acute asthmatic mouse model using
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ANXA3 interference inactivates ERK/ELK1 pathway to mitigate inflammation and apoptosis in sepsis-associated acute lung injury Mol. Immunol. (IF 3.6) Pub Date : 2024-02-03 Jifang Liang, Junkun Zhang, Jixiu Fan, Shuxian Chen, Weidong Wu
Acute lung injury (ALI) is a prevailing and deadly complication of sepsis coupled with increasing incidence and fatality rate. Annexin A3 (ANXA3) has been unraveled to be upregulated during sepsis. This study purposed to assess the role and the mechanism of ANXA3 in sepsis-induced ALI. After the construction of mouse model of sepsis, the pathological changes of mice lung tissues were estimated by H&E
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IFITM3 mediates inflammation induced myocardial injury through JAK2/STAT3 signaling pathway Mol. Immunol. (IF 3.6) Pub Date : 2024-02-01 Chunming Xiong, Bohan Li, Renxing Song, Zizhe Ma, Sally A. Huber, Wei Liu
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α-mangostin derivatives ameliorated mouse DSS-induced chronic colitis via regulating Th17/Treg balance Mol. Immunol. (IF 3.6) Pub Date : 2024-01-26 Yuying Yang, Yuqing Deng, Guoqiang Zhang, Xiaoting Xu, Xiaoxiao Xiong, Si Yu, Fanrong Peng, Xuyan Tian, Weiying Ye, Huanpeng Chen, Bolan Yu, Zhonghua Liu, Xixin He, Zhaofeng Huang
Th17 cell, an important subpopulation of helper T cell, plays an important role in the development of inflammatory bowel disease (IBD) and is thought to be a potential target for the treatment of IBD. In our previous study, we demonstrated that α-mangostin could relieve lupus nephritis via inhibiting Th17 cell function. In our preliminary study, we obtained four derivatives by adding chemical modification
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SNX10 promoted liver IR injury by facilitating macrophage M1 polarization via NLRP3 inflammasome activation Mol. Immunol. (IF 3.6) Pub Date : 2024-01-24 Dongming Wu, Yong Wang, Jian Xu, Dong Wang, Jiawei Zhang, Lijuan Meng, Yuanchang Hu, Ping Wang, Jinde Lin, Shun Zhou
Background Liver ischemia reperfusion (IR) injury is a common cause of liver dysfunction in patients post liver partial resection and liver transplantation. However, the cellular defense mechanisms underlying IR are not well understood. Macrophage mediated sterile inflammation plays critical roles in liver IR injury. Sorting nexin (SNX) 10, a member of the SNX family which functions in regulation of
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Influence of pre-B cell receptor deficiency on the immunoglobulin repertoires in peripheral blood B cells before and after immunization Mol. Immunol. (IF 3.6) Pub Date : 2024-01-24 Takeyuki Shimizu, Lin Sun, Kazuo Ohnishi
During B cell development, pre-B cell receptor (pre-BCR), comprising the immunoglobulin heavy chain (HC) and surrogate light chain (SLC), plays a crucial role. The expression of pre-BCR serves as a certification of HC quality, confirming its ability to associate with the SLC and light chain (LC). In mice lacking SLC, the absence of this quality control mechanism leads to a distorted repertoire of HCs
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Signaling pathways underlying TGF-β mediated suppression of IL-12A gene expression in monocytes Mol. Immunol. (IF 3.6) Pub Date : 2024-01-25 Tetiana Hourani, Mahtab Eivazitork, Thivya Balendran, Kevin MC. Lee, John A. Hamilton, Hong-Jian Zhu, Josephine Iaria, Andrew P. Morokoff, Rodney B. Luwor, Adrian A. Achuthan
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Immunoregulatory function of SP-A Mol. Immunol. (IF 3.6) Pub Date : 2024-01-19 Shu Dong, Hongyuan Pang, Fan Li, Mengqing Hua, Meng Liang, Chuanwang Song
Surfactant protein A (SP-A), a natural immune molecule, plays an important role in lung health. SP-A recognizes and binds microbial surface glycogroups through the C-type carbohydrate recognition domain, and then binds corresponding cell surface receptors (such as C1qRp, CRT-CD91 complex, CD14, SP-R210, Toll-like receptor, SIRP-α, CR3, etc.) through collagen-like region, and subsequently mediates biological
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Investigation of T cell-related hub genes in diabetic nephropathy by bioinformatics analysis and experiment validation Mol. Immunol. (IF 3.6) Pub Date : 2024-01-19 Hongyan Luo, Lirong Yang, Danna Ma, Xi Bao, Guoqing Zhang, Bo Li, Shilu Cao, Shunyao Liu, Li Bao, , Yali Zheng
Diabetic nephropathy(DN) remains a significant risk factor for cardiovascular and all-cause mortality, and end-stage renal disease (ESRD) associated with it is growing in prevalence.However, there is absolutely no curative strategy for DN. We subjected db/db and control mouse kidneys to transcriptional sequencing analysis to obtain transcriptome expression profile data in the diabetic nephropathy.We
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A novel Mycobacterium Tuberculosis antigen, MTB48 enhances inflammatory response in LPS-induced RAW264.7 macrophage immune cells Mol. Immunol. (IF 3.6) Pub Date : 2024-01-17 Jun-Young Park, Hee-Do Kim, Fukushi Abekura, Seung-Hak Cho, Cheorl-Ho Kim
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Cytoprotective effects of C1s enzyme in macrophages in atherosclerosis mediated through the LRP5 and Wnt/β-catenin pathway Mol. Immunol. (IF 3.6) Pub Date : 2024-01-12 Dong Yuan, Zhipeng Zheng, Cheng Shen, Jun Ye, Li Zhu
C1s enzyme (active C1s) is a subunit of the complement C1 complex that cleaves low-density lipoprotein receptor-related proteins 5 and 6, leading to Wnt/β-catenin pathway activation in some cell lines. Macrophages have two major functional polarization states (the classically activated M1 state and the alternatively activated M2 state) and play an essential role in atherosclerosis. An increasing amount
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N-terminal ectodomain of BTNL2 inhibits T cell activation via a non-canonical interaction with its putative receptor that results in a delayed progression of DSS-induced ulcerative colitis Mol. Immunol. (IF 3.6) Pub Date : 2024-01-13 Anita Hansda, Saumyadeep Goswami, Sarbartha Mukherjee, Aditya J. Basak, Shirin Dasgupta, Pritam Kumar Roy, Dibyendu Samanta, Gayatri Mukherjee
Butyrophilin-like 2 (BTNL2) is a T cell inhibitory molecule that interacts with unknown binding partners to modulate the immune response in a number of inflammatory and autoimmune diseases. In this study, we found that the inhibitory effects of BTNL2 on T cell activation and effector functions can be executed by its N-terminal IgV domain (BTNL2 IgV1) alone. Structure-guided mutation of key residues
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HDAC6-MYCN-CXCL3 axis mediates allergic inflammation and is necessary for allergic inflammation-promoted cellular interactions Mol. Immunol. (IF 3.6) Pub Date : 2024-01-03 Yoojung Kwon, Yunji Choi, Misun Kim, Hyein Jo, Myeong Seon Jeong, Hyun Suk Jung, Dooil Jeoung
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Towards detection of structurally-diverse glycated epitopes in native proteins: Single-chain antibody directed to non-A1c epitope in human haemoglobin Mol. Immunol. (IF 3.6) Pub Date : 2024-01-04 Miss Lucia Gaetani, Isabel Campos Pinto, Meng Li, Peter O’Connor, Susan Giorgi-Coll, Matthew Tyreman, Katherine L. Rumary, James A. Schouten, Paul Davis, Ann M. Dixon
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Single-cell transcriptional profiling reveals aberrant gene expression patterns and cell states in autoimmune diseases Mol. Immunol. (IF 3.6) Pub Date : 2023-12-29 Zhenyu Liu, Wujun Wei, Junning Zhang, Xueli Yang, Zhihui Feng, Biao Zhang, Xianliang Hou
Multiple sclerosis(MS), primary Sjögren syndrome (pSS), and systemic lupus erythematosus (SLE) share numerous clinical symptoms and serological characteristics. We analyzed 153550 cells of scRNA-seq data of 17 treatment-naive patients (5 MS, 5 pSS, and 7 SLE) and 10 healthy controls, and we examined the enrichment of biological processes, differentially expressed genes (DEGs), immune cell types, and
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Recombinant human scFv antibody fragments against phospholipase A2 from Naja naja and Echis carinatus snake venoms: In vivo neutralization and mechanistic insights Mol. Immunol. (IF 3.6) Pub Date : 2023-12-27 Amit Kumar, Zaid Kamal Madni, Shivam Chaturvedi, Dinakar M. Salunke
Snake envenomation results in a range of clinical sequelae, and widely used animal-based conventional antivenoms exhibit several limitations including the adverse immunological effects in human snake bite victims. Therefore, human monoclonal anti-snake venom antibodies or fragments can be an alternate therapy for overcoming the existing limitations. We developed venom-neutralizing humanized scFv antibodies
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Inhibition of cGAS-STING pathway by stress granules after activation of M2 macrophages by human mesenchymal stem cells against drug induced liver injury Mol. Immunol. (IF 3.6) Pub Date : 2023-12-27 Yao Wang, Sha She, Wenyuan Li, Jiling Zhu, Xun Li, Fan Yang, Kai Dai
Objective Cells can produce stress granules (SGs) to protect itself from damage under stress. The cGAS-STING pathway is one of the important pattern recognition pathways in the natural immune system. This study was investigated whether human mesenchymal stem cells (hMSCs) could protect the liver by inducing M2 macrophages to produce SGs during acute drug induced liver injury (DILI) induced by acetaminophen
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Characterization of the bispecific VHH antibody gefurulimab (ALXN1720) targeting complement component 5, and designed for low volume subcutaneous administration Mol. Immunol. (IF 3.6) Pub Date : 2023-12-23 Siddharth Jindal, Dennis Vestergaard Pedersen, Nimish Gera, Julian Chandler, Rekha Patel, Alyssa Neill, Josh Cone, Yuchun Zhang, Chao-Xing Yuan, Ellen E. Millman, Dan Carlin, Bridget Puffer, Douglas Sheridan, Gregers Rom Andersen, Paul Tamburini
The bispecific antibody gefurulimab (also known as ALXN1720) was developed to provide patients with a subcutaneous treatment option for chronic disorders involving activation of the terminal complement pathway. Gefurulimab blocks the enzymatic cleavage of complement component 5 (C5) into the biologically active C5a and C5b fragments, which triggers activation of the terminal complement cascade. Heavy-chain
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Corrigendum to: “Identification of an AnnexinB9 involve in white spot syndrome virus infection in red claw crayfish Cherax quadricarinatus” [Mol. Immunol. 162 (2023) 21–29] Mol. Immunol. (IF 3.6) Pub Date : 2023-12-21 Shan Liu, Tengping Jiang, Lei Dai
Abstract not available
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The SpyCatcher-SpyTag interaction mediates tunable anti-tumor cytotoxicity of NK cells Mol. Immunol. (IF 3.6) Pub Date : 2023-12-20 Changjiang Guo, Xiali Guo, Xiaojuan Li, Meng Dong, Xiang Wang, Shizhuang Cheng, Lingtong Zhi, Zhiyuan Niu, Wuling Zhu
Chimeric antigen receptor (CAR)-modified T and NK cell immunotherapy is a promising approach for cancer treatment. Due to the lack of tunability in anti-tumor activity, conventional CAR therapies have limited efficacy at low tumor antigen densities. To tune the CAR response to tumor cell surface antigens, we have developed a split CAR using the SpyCatcher-SpyTag system. The SpyCatcher serves as the
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B7-H3 promotes angiogenesis in rheumatoid arthritis Mol. Immunol. (IF 3.6) Pub Date : 2023-12-21 Jie Yang, Jian Xiong, Yuling Sun, Li Gu, Yachun Chen, Yundi Guo, Cuiping Liu, Jing Sun
Objective The primary pathological changes of rheumatoid arthritis (RA) include chronic synovial inflammation, bone destruction, and aggressive pannus formation on cartilage, in which angiogenesis plays a critical role. B7-H3, an important immune checkpoint molecule, represents a novel target in tumor therapy and plays a significant role in the pathogenesis of autoimmune diseases. However, its biological
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Mosquito allergy: Immunological aspects and clinical management Mol. Immunol. (IF 3.6) Pub Date : 2023-11-30 Jose Fernando Cantillo, Elizabeth Garcia, Enrique Fernandez-Caldas, Leonardo Puerta
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Corrigendum to “DNA damage-triggered activation of cGAS-STING pathway induces apoptosis in human keratinocyte HaCaT cells” [Mol. Immunol. 131 (2021) 6222] Mol. Immunol. (IF 3.6) Pub Date : 2023-11-24 Can Li, Weiwei Liu, Fang Wang, Toshihiko Hayashi, Kazunori Mizuno, Shunji Hattori, Hitomi Fujisaki, Takashi Ikejima
Abstract not available
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Immunomodulation by mesenchymal stem cells during osteogenic differentiation: Clinical implications during bone regeneration Mol. Immunol. (IF 3.6) Pub Date : 2023-11-26 Aditi Mahajan, Shalmoli Bhattacharyya
Critical bone defects resulting in delayed and non-union are a major concern in the field of orthopedics. Over the past decade, mesenchymal stem cells (MSCs) have become a promising frontier for bone repair and regeneration owing to their high expansion rate and osteogenic differentiation potential ex vivo. MSCs have also long been associated with their ability to modulate immune response in the recipients
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Molecular cloning of the hepcidin gene from crescent sweetlips (Plectorhinchus cinctus) and characterization of its encoded antimicrobial peptide Mol. Immunol. (IF 3.6) Pub Date : 2023-11-24 Peixin Wang, Zhongjing Lin, Shaoling Lin, Yujie Dai, Baodong Zheng, Yi Zhang, Jiamiao Hu
Hepcidin has been identified as an important antimicrobial peptide exerting important innate immunomodulatory activities in many fish species. In the present study, reverse transcription PCR coupled with the rapid amplification of cDNA ends was used to obtain the full-length cDNA of the crescent sweetlips hepcidin gene, which is 829 bp in length and includes an 273 bp ORF encoding a peptide with 90
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Identification and functional characterization of Caspase-9 in goldfish (Carassius auratus L.) in response to Aeromonas hydrophila infection Mol. Immunol. (IF 3.6) Pub Date : 2023-11-25 Xinyuan Yue, Jingyi Liu, Ziqi Ban, Xionglin Li, Jianhu Jiang, Jiasong Xie
Apoptosis plays a pivotal role in the immune response to combat pathogen infections. In mammals, caspase-9, abbreviated as Casp9, plays an irreplaceable role in the initiation phase of the apoptotic cascade. To investigate the role of Casp9 in teleosts, we conducted a functional characterization of Casp9 in goldfish (Carassius auratus L.). The open reading frame of GfCasp9 spans 1296 base pairs (bp)
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Interleukins-6 −174G/C (rs1800795) and −572C/G (rs1800796) polymorphisms and prostate cancer risk Mol. Immunol. (IF 3.6) Pub Date : 2023-11-20 Muhammad Sarfaraz Iqbal, Kaoqing Peng, Nimra Sardar, Muhammad Hasnain Iqbal, Muhammad Usman Ghani, Fouzia Tanvir, Di Gu, Zeng Guohua, Xiaolu Duan
Prostate cancer (PCa) is an aggressive cancer influenced by a complex interplay of genetic and environmental factors. Amongst these risk factors, the impact of Interleukin6 (IL6) gene polymorphisms in PCa risk has received a lot of attention. IL-6 is a cytokine that has been implicated in the pathogenesis of several malignancies, including PCa. Two IL-6 gene polymorphisms, − 174 G/C (rs1800795) and