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Overexpression of YTHDF3 increases the specific productivity of the recombinant protein in CHO cells by promoting the translation process Biotechnol. J. (IF 4.7) Pub Date : 2024-04-23 Zhao‐ming Cui, Ying‐ying Feng, Yan‐ping Gao, Hai‐tong Wang, Jiang‐tao Lu, Jia‐liang Guo, Hong‐yan Xu, Le‐le Qiu, Tian‐yun Wang, Yan‐long Jia
Due to their high‐quality characteristics, Chinese hamster ovary (CHO) cells have become the most widely used and reliable host cells for the production of recombinant therapeutic proteins in the biomedical field. Previous studies have shown that the m6A reader YTHDF3, which contains the YTH domain, can affect a variety of biological processes by regulating the translation and stability of target mRNAs
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Development of an efficient liposomal DOX delivery formulation for HCC therapy by targeting CK2α Biotechnol. J. (IF 4.7) Pub Date : 2024-04-23 Ruixia Zhao, Sinan Cheng, Xue Bai, Danying Zhang, Hongming Fang, Wanlin Che, Wenxuan Zhang, Yujuan Zhou, Wei Duan, Qiumin Liang, Li Xiao, Guochao Nie, Yingchun Hou
Hepatocellular carcinoma (HCC) is a digestive tract cancer with high mortality and poor prognosis, especially in China. Current chemotherapeutic drugs lead to poor prognosis, low efficacy, and high side effects due to weak targeting specificity and rapidly formed multidrug resistance (MDR). Based on the previous studies on the doxorubicin (DOX) formulation for cancer targeting therapy, we developed
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Tuning CpG motif position in nanostructured DNA for efficient immune stimulation Biotechnol. J. (IF 4.7) Pub Date : 2024-04-23 Mengmeng Tan, Natsuki Makiguchi, Kosuke Kusamori, Shoko Itakura, Yuki Takahashi, Yoshinobu Takakura, Makiya Nishikawa
It was previously demonstrated that polypod‐like nanostructured DNA (polypodna) comprising three or more oligodeoxynucleotides (ODNs) were useful for the delivery of ODNs containing cytosine–phosphate–guanine (CpG) motifs, or CpG ODNs, to immune cells. Although the immunostimulatory activity of single‐stranded CpG ODNs is highly dependent on CpG motif sequence and position, little is known about how
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Deinococcus wulumuqiensis R12 synthesized silver nanoparticles with peroxidase‐like activity for synergistic antibacterial application Biotechnol. J. (IF 4.7) Pub Date : 2024-04-23 Jingjia Liu, Nan Zhang, Bowen Shen, Liling Zhang, Zhidong Zhang, Liying Zhu, Ling Jiang
The use of a combination of several antibacterial agents for therapy holds great promise in reducing the dosage and side effects of these agents, improving their efficiency, and inducing potential synergistic therapeutic effects. Herein, this study provides an innovative antibacterial treatment strategy by synergistically combining R12‐AgNPs with H2O2 therapy. R12‐AgNPs were simply produced with the
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Accelerated generation of gene‐engineered monoclonal CHO cell lines using FluidFM nanoinjection and CRISPR/Cas9 Biotechnol. J. (IF 4.7) Pub Date : 2024-04-23 Justin S. Antony, Anabel Migenda Herranz, Tahereh Mohammadian Gol, Susanne Mailand, Paul Monnier, Jennifer Rottenberger, Alicia Roig‐Merino, Bettina Keller, Claudia Gowin, Maria Milla, Tobias A. Beyer, Markus Mezger
Chinese hamster ovary (CHO) cells are the commonly used mammalian host system to manufacture recombinant proteins including monoclonal antibodies. However unfavorable non‐human glycoprofile displayed on CHO‐produced monoclonal antibodies have negative impacts on product quality, pharmacokinetics, and therapeutic efficiency. Glycoengineering such as genetic elimination of genes involved in glycosylation
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Phase separation methods for protein purification: A meta‐analysis of purification performance and cost‐effectiveness Biotechnol. J. (IF 4.7) Pub Date : 2024-04-23 John S. Decker, Utsuki Yano, Romel Menacho Melgar, Michael D. Lynch
Protein purifications based on phase separations (e.g., precipitation and liquid‐liquid extraction) have seen little adoption in commercial protein drug production. To identify barriers, we analyzed the purification performance and economics of 290 phase separation purifications from 168 publications. First, we found that studies using Design of Experiments for optimization achieved significantly greater
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Biological autoluminescence enables effective monitoring of yeast cell electroporation Biotechnol. J. (IF 4.7) Pub Date : 2024-04-23 Martin Bereta, Michal Teplan, Tomáš Zakar, Hoang Vuviet, Michal Cifra, Djamel Eddine Chafai
The application of pulsed electric fields (PEFs) is becoming a promising tool for application in biotechnology, and the food industry. However, real‐time monitoring of the efficiency of PEF treatment conditions is challenging, especially at the industrial scale and in continuous production conditions. To overcome this challenge, we have developed a straightforward setup capable of real‐time detection
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A new look at the fluorescent protein‐based approach for identifying optimal coding sequence for recombinant protein expression in E. coli Biotechnol. J. (IF 4.7) Pub Date : 2024-04-16 Artur I. Zabolotskii, Natalia S. Riabkova
Due to the degeneracy of the genetic code, most amino acids are encoded by several codons. The choice among synonymous codons at the N‐terminus of genes has a profound effect on protein expression in Escherichia coli. This is often explained by the different contributions of synonymous codons to mRNA secondary structure formation. Strong secondary structures at the 5'‐end of mRNA interfere with ribosome
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Rainbow screening: Chromoproteins enable visualized molecular cloning Biotechnol. J. (IF 4.7) Pub Date : 2024-04-16 Fang Ba, Yufei Zhang, Wan‐Qiu Liu, Jian Li
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Fluid shear force and hydrostatic pressure jointly promote osteogenic differentiation of BMSCs by activating YAP1 and NFAT2 Biotechnol. J. (IF 4.7) Pub Date : 2024-04-16 Yi Zhou, Pan Guo, Ziyang Jin, Miaomiao Chai, Shuhong Zhang, Xianwei Wang, Wen‐Song Tan, Yan Zhou
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Recent advances in the synthesis of single‐stranded DNA in vitro Biotechnol. J. (IF 4.7) Pub Date : 2024-04-16 Shuai Li, Wei Tan, Xuemei Jia, Qing Miao, Ying Liu, Dayong Yang
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Depolymerization of the polyester–polyurethane by amidase GatA250 and enhancing the production of 4,4′‐methylenedianiline with cutinase LCC Biotechnol. J. (IF 4.7) Pub Date : 2024-04-16 Kaiyuan Xin, Jianqi Lu, Qingyi Zeng, Tianyang Zhang, Jiawei Liu, Jie Zhou, Weiliang Dong, Min Jiang
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Visual screening of CRISPR/Cas9 editing efficiency based on micropattern arrays for editing porcine cells Biotechnol. J. (IF 4.7) Pub Date : 2024-04-16 Wanliu Peng, Mengyu Gao, Xinglong Zhu, Xinmei Liu, Guang Yang, Shun Li, Yong Liu, Lang Bai, Jiayin Yang, Ji Bao
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Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases Biotechnol. J. (IF 4.7) Pub Date : 2024-04-09 Konstantinos Makryniotis, Efstratios Nikolaivits, George Taxeidis, Jasmina Nikodinovic-Runic, Evangelos Topakas
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Feeding strategies for Sporosarcina pasteurii cultivation unlock more efficient production of ureolytic biomass for MICP Biotechnol. J. (IF 4.7) Pub Date : 2024-04-06 Frédéric M. Lapierre, Robert Huber
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Unveiling the mechanism of efficient β‐phenylethyl alcohol conversion in wild‐type Saccharomyces cerevisiae WY319 through multi‐omics analysis Biotechnol. J. (IF 4.7) Pub Date : 2024-04-06 Chenghan Yang, Yilin Ren, Lihao Ge, Wenting Xu, Haifeng Hang, Ali Mohsin, Xiwei Tian, Ju Chu, Yingping Zhuang
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Proteomic analysis reveals that the co‐ordination of cytosolic and mitochondrial pathways is beneficial for sabinene biosynthesis in engineered Saccharomyces cerevisiae Biotechnol. J. (IF 4.7) Pub Date : 2024-04-06 Rui Hou, Mengying Shan, Xinxin Liu, Mingdong Yao, Kaiguang Yang, Ying Wang, Zhigang Sui, Zhen Liang, Yukui Zhang, Lihua Zhang
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Transcriptomic analysis provides an insight into the function of CmGH9B3, a key gene of β‐1, 4‐glucanase, during the graft union healing of oriental melon scion grafted onto squash rootstock Biotechnol. J. (IF 4.7) Pub Date : 2024-04-06 Yulei Zhu, Shengwei Hu, Jiahuan Min, Yingtong Zhao, Hanqi Yu, Muhammad Irfan, Chuanqiang Xu
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Production of ultra‐high‐molecular‐weight poly‐γ‐glutamic acid by a newly isolated Bacillus subtilis strain and genomic and transcriptomic analyses Biotechnol. J. (IF 4.7) Pub Date : 2024-04-06 Wei Zeng, Yuanyuan Liu, Lin Shu, Yin Guo, Linye Wang, Zhiqun Liang
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Cascade reactions with two non‐physiological partners for NAD(P)H regeneration via renewable hydrogen Biotechnol. J. (IF 4.7) Pub Date : 2024-04-06 Francisco Gasteazoro, Gianluca Catucci, Lisa Barbieri, Melissa De Angelis, Alessandro Dalla Costa, Sheila J. Sadeghi, Gianfranco Gilardi, Francesca Valetti
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Tri‐enzyme fusion of tryptophan halogenase achieves a concise strategy for coenzyme self‐sufficiency and the continuous halogenation of L‐tryptophan Biotechnol. J. (IF 4.7) Pub Date : 2024-04-06 Han‐Yu Liu, Feng Qian, Hai‐Min Zhang, Qian Gui, Yao‐Wu Wang, Pu Wang
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R2D ligase: Unveiling a novel DNA ligase with surprising DNA‐to‐RNA ligation activity Biotechnol. J. (IF 4.7) Pub Date : 2024-03-26 Sigurd Eidem Gundesø, Ulli Rothweiler, Elise Heimland, Yvonne Piotrowski, Inger Kristine Rødum, Jenny Johansson Söderberg, Ildikó Moustaka Gábor, Terese Solstad, Adele Williamson, Olav Lanes, Bernd Ketelsen Striberny
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Development of synthetic small regulatory RNA for Rhodococcus erythropolis Biotechnol. J. (IF 4.7) Pub Date : 2024-03-26 Lijuan Wang, Jie Hou, Kun Yang, Haonan Yu, Bo Zhang, Zhiqiang Liu, Yuguo Zheng
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An innovative hybrid modeling approach for simultaneous prediction of cell culture process dynamics and product quality Biotechnol. J. (IF 4.7) Pub Date : 2024-03-26 Jakub Polak, Zhuangrong Huang, Michael Sokolov, Moritz von Stosch, Alessandro Butté, C. Eric Hodgman, Michael Borys, Anurag Khetan
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Fc engineered anti‐virus therapeutic human IgG1 expressed in plants with altered binding to the neonatal Fc receptor Biotechnol. J. (IF 4.7) Pub Date : 2024-03-26 Sol‐Ah Park, Yoonji Lee, Hyunjoo Hwang, Jeong Hwan Lee, Yang Joo Kang, Yerin Kim, Caiquan Jin, Hyun Joo An, Yoo Jin Oh, Peter Hinterdorfer, Eunhye Kim, Sun Choi, Kisung Ko
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Construction and characterization of a functional variant hFGF7 with enhanced properties by circular permutation Biotechnol. J. (IF 4.7) Pub Date : 2024-03-26 Hye‐Ji Choi, Hanui Lee, Dae‐Eun Cheong, Su‐Kyoung Yoo, Dong‐Eun Lee, Geun‐Joong Kim
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Characterization of cellular responses and cell lysis to elevated hydrodynamic stress from benchtop perfusion bioreactors Biotechnol. J. (IF 4.7) Pub Date : 2024-03-26 Weijian Zhang, Qingyuan Ran, Liang Zhao, Qian Ye, Wen‐Song Tan
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Evaluation of in vitro corrosion behavior and biocompatibility of poly[xylitol‐(1,12‐dodecanedioate)](PXDD)‐HA coated porous iron for bone scaffolds applications Biotechnol. J. (IF 4.7) Pub Date : 2024-03-21 Abdul Hakim Md Yusop, Wan Fahmin Faiz Wan Ali, Farah Hidayah Jamaludin, Fatihhi Szali Januddi, Murni Nazira Sarian, Norazalina Saad, Tuck‐Whye Wong, Arif Hidayat, Hadi Nur
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Proteomic workflows for deep phenotypic profiling of 3D organotypic liver models Biotechnol. J. (IF 4.7) Pub Date : 2024-03-21 Stefania Koutsilieri, Evgeniya Mickols, Ákos Végvári, Volker M. Lauschke
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Design of a cofactor self‐sufficient whole‐cell biocatalyst for enzymatic asymmetric reduction via engineered metabolic pathways and multi‐enzyme cascade Biotechnol. J. (IF 4.7) Pub Date : 2024-03-21 Shuping Zou, Bing Zhang, Yuyue Han, Jinlong Liu, Kuo Zhao, Yaping Xue, Yuguo Zheng
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Highly chromophoric fluorescent‐labeled methionyl‐initiator tRNAs applicable in living cells Biotechnol. J. (IF 4.7) Pub Date : 2024-03-18 Jung Min Kim, Jinho Jung
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Recent advances and fundamentals of microalgae cultivation technology Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Julian M. Rozenberg, Boris A. Sorokin, Amina N. Mukhambetova, Anna A. Emelianova, Valentin V. Kuzmin, Oxana Y. Belogurova‐Ovchinnikova, Denis V. Kuzmin
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Rigid linker peptides improve the stability and anti‐inflammation effect of human serum albumin and α‐melanocyte‐stimulating hormone fusion proteins Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Yiyao Liu, Yang Li, Xueyan Wei, Inam Ullah, Shahab Uddin, Jiatao Wang, Runjie Xia, MeiZhu Wang, Hui Yang, Hongyu Li
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Predicting protein retention in ion‐exchange chromatography using an open source QSPR workflow Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Tim Neijenhuis, Olivier Le Bussy, Geoffroy Geldhof, Marieke E. Klijn, Marcel Ottens
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Modification of nitrilase based on computer screening and efficient biosynthesis of 4‐cyanobenzoic acid Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Yi‐Zhong Jin, Ji‐Dong Shen, Yu‐xin Wang, Hong‐Ming Shao, Hai‐Jian Xia, Jiao‐Hua Lin, Zhi‐Qiang Liu, Yu‐Guo Zheng
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Enhanced targeting: Production of filamentous bacteriophage monoclonal antibodies using the major coat protein pVIII as anchor Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Qing Li, Jianyu Zhu, Ting Yuan, Leina Dou, Xuezhi Yu, Jianzhong Shen, Huijuan Yang, Zhanhui Wang
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Tuning capsid formation dynamics in recombinant adeno‐associated virus producing synthetic cell lines to enhance full particle productivity Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Min Lu, Yu‐Chieh Lin, Han‐Jung Kuo, Wen Cai, Qian Ye, Liang Zhao, Wei‐Shou Hu
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Enhanced synthesis of S‐adenosyl‐L‐methionine through combinatorial metabolic engineering and Bayesian optimization in Saccharomyces cerevisiae Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Wenhan Xiao, Xiangliu Shi, Haowei Huang, Xiaogang Wang, Wenshu Liang, Jianguo Xu, Fei Liu, Xiaomei Zhang, Guoqiang Xu, Jinsong Shi, Zhenghong Xu
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Promoter screening and identification for metabolic regulation in Acremonium chrysogenum Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Ling Liu, Runge Li, Xinrun Zhang, Zhen Chen, Ali Mohsin, Haifeng Hang, Xiwei Tian, Ju Chu
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Enhancing the production of adeno‐associated virus (AAV)2 and AAV9 with high full capsid ratio in HEK293 cells through design‐of‐experiment optimization of triple plasmid ratio Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Sungje Park, Seunghyeon Shin, Haeshin Lee, Jae‐Hyung Jang, Gyun Min Lee
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Standardized approach for accurate and reliable model development of ion‐exchange chromatography based on parameter‐by‐parameter method and consideration of extra‐column effects Biotechnol. J. (IF 4.7) Pub Date : 2024-03-14 Yu‐Cheng Chen, Hui‐Li Lu, Rong‐Zhu Wang, Guo Sun, Xue‐Qin Zhang, Jing‐Qi Liang, Alois Jungbauer, Shan‐Jing Yao, Dong‐Qiang Lin
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Combining directed evolution with high cell permeability for high‐level cadaverine production in engineered Escherichia coli Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Xuemei Liu, Ruoshi Luo, Dan Wang, Kaixing Xiao, Fanzhen Lin, Ya Qi Kang, Xue Xia, Xiaojie Zhou, Ge Hu
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Computer‐aided design to enhance the stability of aldo‐keto reductase KdAKR Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Chen Dai, Jia‐Xin Tian, Yu‐Feng Chen, Yue‐Han Ni, Lei Cui, Hai‐Xing Cao, Lin‐Lin Song, Shen‐Yuan Xu, Ya‐Jun Wang, Yu‐Guo Zheng
The aldo‐keto reductase (AKR) KdAKR from Kluyvermyces dobzhanskii can reduce t‐butyl 6‐chloro‐(5S)‐hydroxy‐3‐oxohexanoate ((5S)‐CHOH) to t‐butyl 6‐chloro‐(3R,5S)‐dihydroxyhexanoate ((3R,5S)‐CDHH), which is the key chiral intermediate of rosuvastatin. Herein, a computer‐aided design that combined the use of PROSS platform and consensus design was employed to improve the stability of a previously constructed
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A single‐cell genomic strategy for alternative transcript start sites identification Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Yanling Peng, Qitong Huang, Danli Liu, Siyuan Kong, Rui Kamada, Keiko Ozato, Yubo Zhang, Jun Zhu
Alternative transcription start sites (TSSs) usage plays a critical role in gene transcription regulation in mammals. However, precisely identifying alternative TSSs remains challenging at the genome‐wide level. We report a single‐cell genomic technology for alternative TSSs annotation and cell heterogeneity detection. In the method, we utilize Fluidigm C1 system to capture individual cells of interest
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Enhancing short‐chain fatty acids production via acidogenic fermentation of municipal sewage sludge: Effect of sludge characteristics and peroxydisulfate pre‐oxidation Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Faxin Liu, Wen Cheng, Jianping Xu, Tian Wan, Min Wang, Jiehui Ren, Maomao Ning, Hui Zhang, Xiaoping Zhou
This study first employed a combined pretreatment of low‐dose peroxy‐disulfate (PDS) and initial pH 10 to promote short‐chain fatty acids (SCFAs) production via acidogenic fermentation using different types of sewage sludge as substrates. The experimental results showed that the yield of maximal SCFAs and acetate proportion after the combined pretreatment were 1513.82 ± 28.25 mg chemical oxygen demand
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Evaluation of the cytotoxic activity of sorafenib‐loaded camel milk casein nanoparticles against hepatocarcinoma cells Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Aastha Mittal, Neelam Mahala, Nikhil Hanamant Dhanawade, Sunil Kumar Dubey, Uma S. Dubey
Sorafenib, a multikinase inhibitor is used to treat hepatocellular and renal carcinoma. However, a low solubility impedes its bioavailability and thus, effectiveness. This study aims to enhance its effectiveness by using novel camel milk casein nanoparticles as a delivery system. This study evaluates the cytotoxicity of sorafenib encapsulated in camel milk casein nanoparticles against human hepatocarcinoma
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High yield sterile filtration process for highly concentrated lentiviral vectors Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Jessica E. Vogel, Monica Terrao, Sarah Schwingal, Laura Kapitza, Daniel Brigulla, Vicky Pirzas, Holger Laux, Tobias Brandt
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Octadecyl and sulfonyl modification of diatomite synergistically improved the immobilization efficiency of lipase and its application in the synthesis of pine sterol esters Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Yifei Zhang, Wei Zhang, Guangzheng Ma, Binbin Nian, Yi Hu
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Lipidomic and transcriptomic analysis of the increase in eicosapentaenoic acid under cobalamin deficiency of Schizochytrium sp Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Lin‐Hui Yang, Meng‐zhen Liu, Zi‐Lei Chen, Ling‐Ling Tong, Dong‐Sheng Guo
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Human proinsulin production in the milk of transgenic cattle Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Paulo S. Monzani, Juliano R. Sangalli, Rafael V. Sampaio, Samuel Guemra, Renato Zanin, Paulo R. Adona, Maria A. Berlingieri, Luiz F. C. Cunha‐Filho, Irma Y. Mora‐ Ocampo, Carlos P. Pirovani, Flávio V. Meirelles, Matthew B. Wheeler, Otavio M. Ohashi
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Suspension culture promoted the expansion of NK‐92 cells ex vivo by enhancing the expression of IL‐2 receptor Biotechnol. J. (IF 4.7) Pub Date : 2024-03-13 Huimin Huang, Shumin Zhang, Yuanyuan Zhao, Ruisheng Xu, Wen‐song Tan, Haibo Cai
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A large‐scale production of mesenchymal stem cells and their exosomes for an efficient treatment against lung inflammation Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Jinsong Zhang, Ruyi Lin, Yingyu Li, Jiawen Wang, Huiqing Ding, Panfeng Fang, Yingzhi Huang, Jing Shi, Jianqing Gao, Tianyuan Zhang
Mesenchymal stem cells (MSCs) and their produced exosomes have demonstrated inherent capabilities of inflammation‐guided targeting and inflammatory modulation, inspiring their potential applications as biologic agents for inflammatory treatments. However, the clinical applications of stem cell therapies are currently restricted by several challenges, and one of them is the mass production of stem cells
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Encapsulation of pristine and silica‐coated human adipose‐derived mesenchymal stem cells in gelatin colloidal hydrogels for tissue engineering and bioprinting applications Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Marta M. Maciel, Negar Hassani Besheli, Tiago R. Correia, João F. Mano, Sander C. G. Leeuwenburgh
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Lung‐specific exosomes for doxorubicin delivery in lung adenocarcinoma therapy Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Fengqiang Yu, Yanxun Chen, Weiqiang Yi, Maohao Guan, Nanlong Lin, Yi Zhuo, Jianbo Lin, Fancai Lai
Doxorubicin (DOX) could be utilized to treat lung adenocarcinoma (LUAD), while dose‐limiting cardiotoxicity limits its clinical utilization. MDA‐MB‐231 cell‐derived exosomes show lung‐specific organotropism features. In this study, we aimed to explore the potential of MDA‐MB‐231 cell‐derived exosomes in DOX specific delivery to the lung. MDA‐MB‐231 cell‐derived exosomes were coincubated with to construct
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A refined Uni‐vector prime editing system improves genome editing outcomes in mammalian cells Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Ching‐Hui Huang, Szu‐Ying Chiu, Yu‐Chi Chou, Kou‐Juey Wu
Prime editing is an advanced technology in CRISPR/Cas research with increasing numbers of improved methodologies. The original multi‐vector method hampers the efficiency and precision of prime editing and also has inherent difficulty in generating homozygous mutations in mammalian cells. To overcome these technical issues, we developed a Uni‐vector prime editing system, wherein the major components
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Engineering cannabinoid production in Saccharomyces cerevisiae Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Christina Schmidt, Marco Aras, Oliver Kayser
Phytocannabinoids are natural products with highly interesting pharmacological properties mainly produced by plants. The production of cannabinoids in a heterologous host system has gained interest in recent years as a promising alternative to production from plant material. However, the systems reported so far do not achieve industrially relevant titers, highlighting the need for alternative systems
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Overexpression of Klotho gene using CRISPR/Cas9 induces apoptosis and inhibits cell motility in the human colorectal cancer cells Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Merve Nur Soykan, Sibel Gunes
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Effect of extracellular matrices on production and potency of mesenchymal stem cell‐derived exosomes Biotechnol. J. (IF 4.7) Pub Date : 2024-02-26 Rittika Somadder, Lina Faraj, Saurav Datta, Mathumai Kanapathipillai, Gargi Ghosh