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  • Electricity generation and microbial community in long-running microbial fuel cell for high-salinity mustard tuber wastewater treatment
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-11-16
    Linfang Zhang, Guokai Fu, Zhi Zhang

    High-salinity mustard tuber wastewater (MTWW) was utilized to obtain effluent treatment and recover bio-energy simultaneously in a microbial fuel cell (MFC). Since the long-term performance of MFCs in MTWW remains unclear, this study examined electricity generation and the microbial community that developed over a 195-day test. The MFC produced electricity over the entire testing period, with stable power output obtained on days 32–120. In the stable phase, a maximum power density of 12.43 W·m−3 was observed, and the internal resistance, open circuit voltage, and columbic efficiency (CE) were 148 Ω, 756 mV and 36.7 ± 1.2%, respectively. Chemical Oxygen Demand (COD) and Biochemical Oxygen Demand (BOD) removal continuously increased to 89.0 ± 1.5% and 98.6 ± 2.0%, respectively, the maximum rates that were obtained at the end of the experiment, respectively. In addition, 16S rRNA gene sequencing analysis showed that hydrolytic/fermentative bacteria could be considered as the bioanode core microbiome, constituting 36.90% of the microbiome. Sulfate-reducing bacteria (SRB), including Dethiosulfovibrio, Thermovirga, Desulfovibrio, and Desulfuromonas, eventually outcompeted the exoelectrogens completely, causing an irreversible loss in CE. This study provides more ideas for treatment and utilization of high-salinity MTWW.

    更新日期:2018-11-17
  • Electroautotrophy of Thioalkalivibrio nitratireducens
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-11-16
    Mickaël Rimboud, Wafa Achouak

    The electrochemical behavior and electro-autotrophy of a halo-alkaliphilic chemo-autotrophic Ectothiorhodospiraceae isolated from a soda lake, Thioalkalivibrio nitratireducens, were investigated using electrochemical methods and confocal fluorescence microscopy. The electrocatalysis of oxygen reduction was observed at −0.25 V/Ag/AgCl (−0.055 V/ SHE) with bioelectrodes polarized at −0.3 V/Ag/AgCl (−0.105 V/SHE), displaying a maximum catalytic current density of −620 mA m−2 (voltammetry). No catalytic signal toward nitrate reduction was observed under anaerobia. The microscopic observation of the polarized electrodes compared to non-polarized ones, however, revealed a bacterial proliferation both under aerobic and anaerobic conditions, demonstrating the ability of Tv. nitratireducens to grow with the polarized electrode as sole electron source.

    更新日期:2018-11-17
  • Interaction of prednisone with dsDNA at silver nanoparticles/poly(glyoxal-bis(2-hydroxyanil))/dsDNA modified electrode and its analytical application
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-11-16
    Gözde Aydoğdu Tığ, Derya Koyuncu Zeybek, Bülent Zeybek, Şule Pekyardımcı

    This paper reports the fabrication of an electrochemical DNA biosensor for the electrochemical determination of prednisone (PRD), which is a synthetic corticosteroid. For this purpose, silver nanoparticles (AgNPs) and a new polymer film poly(glyoxal-bis(2-hydroxyanil)) (P(GBHA)) were electrochemically deposited on a glassy carbon electrode (GCE), respectively. Then, an electrochemical DNA biosensor was prepared onto this electrode surface (GCE/AgNPs/P(GBHA)) by the immobilization of dsDNA using a chronoamperometry method. The proposed electrode was characterized by FESEM, XPS, and cyclic voltammetry (CV). The interaction between the PRD and dsDNA immobilized on the GCE/AgNPs/P(GBHA) electrode was investigated via a differential pulse voltammetry (DPV) method and UV–Vis spectrophotometry. The experimental factors affecting the interaction between the PRD concentration and dsDNA were optimized. The fabricated biosensor showed a wide linear response in a PRD concentration range of 1.0–50.0 μg mL−1 depending on both the adenine and guanine base signals. The detection limit based on the guanine and adenine signals was 0.3 μg mL−1 and 0.25 μg mL−1, respectively. The sensor exhibited excellent anti-interferential ability, good stability and reproducibility and was satisfactorily employed for the electrochemical assay of PRD in serum samples. The new DNA biosensor can be utilized for the sensitive, accurate and rapid analysis of PRD.

    更新日期:2018-11-17
  • Capacitively coupled electrical stimulation of rat chondroepiphysis explants: A histomorphometric analysis
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-11-08
    J.J. Vaca-González, J.F. Escobar, J.M. Guevara, Y.A. Hata, G. Gallego Ferrer, D.A. Garzón-Alvarado

    The growth plate is a cartilaginous layer present from the gestation period until the end of puberty where it ossifies joining diaphysis and epiphysis. During this period several endocrine, autocrine, and paracrine processes within the growth plate are carried out by chondrocytes; therefore, a disruption in cellular functions may lead to pathologies affecting bone development. It is known that electric fields impact the growth plate; however, parameters such as stimulation time and electric field intensity are not well documented. Accordingly, this study presents a histomorphometrical framework to assess the effect of electric fields on chondroepiphysis explants. Bones were stimulated with 3.5 and 7 mV/cm, and for each electric field two exposure times were tested for 30 days (30 min and 1 h). Results evidenced that electric fields increased the hypertrophic zones compared with controls. In addition, a stimulation of 3.5 mV/cm applied for 1 h preserved the columnar cell density and its orientation. Moreover, a pre-hypertrophy differentiation in the center of the chondroepiphysis was observed when explants were stimulated during 1 h with both electric fields. These findings allow the understanding of the effect of electrical stimulation over growth plate organization and how the stimulation modifies chondrocytes morphophysiology.

    更新日期:2018-11-09
  • Coalesced thermal and electrotransfer mediated delivery of plasmid DNA to the skin
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-10-23
    Anna Bulysheva, James Hornef, Chelsea Edelblute, Chunqi Jiang, Karl Schoenbach, Cathryn Lundberg, Muhammad Arif Malik, Richard Heller

    Efficient gene delivery and expression in the skin can be a promising minimally invasive technique for therapeutic clinical applications for immunotherapy, vaccinations, wound healing, cancer, and peripheral artery disease. One of the challenges for efficient gene electrotransfer (GET) to skin in vivo is confinement of expression to the epithelium. Another challenge involves tissue damage. Optimizing gene expression profiles, while minimizing tissue damage are necessary for therapeutic applications. Previously, we established that heating pretreatment to 43 °C enhances GET in vitro. We observed a similar trend in vivo, with an IR-pretreatment for skin heating prior to GET. Currently, we tested a range of GET conditions in vivo in guinea pigs with and without preheating the skin to ~43 °C. IR-laser heating and conduction heating were tested in conjunction with GET. In vivo electrotransfer to the skin by moderately elevating tissue temperature can lead to enhanced gene expression, as well as achieve gene transfer in epidermal, dermal, hypodermal and muscle tissue layers.

    更新日期:2018-10-23
  • Functionalized carbon nanotube adsorption interfaces for electron transfer studies of galactose oxidase
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-10-19
    Mulugeta B. Wayu, Michael J. Pannell, Najwa Labban, William S. Case, Julie A. Pollock, Michael C. Leopold
    更新日期:2018-10-22
  • Bioelectrochemical Nitrogen fixation (e-BNF): Electro-stimulation of enriched biofilm communities drives autotrophic nitrogen and carbon fixation
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-10-16
    Laura Rago, Sarah Zecchin, Federica Villa, Andrea Goglio, Anna Corsini, Lucia Cavalca, Andrea Schievano

    A new approach to microbial electrosynthesis is proposed, aimed at producing whole biomass from N2 and inorganic carbon, by electrostimulation of complex microbial communities. On a carbon-based conductor under constant polarization (−0.7 V vs SHE), an electroactive biofilm was enriched with autotrophic nitrogen fixing microorganims and led to biomass synthesis at higher amounts (up to 18 fold), as compared to controls kept at open circuit (OC). After 110 days, the electron transfer had increased by 30-fold, as compared to abiotic conditions. Metagenomics evidenced Nif genes associated with autotrophs (both Archaea and Bacteria) only in polarized biofilms, but not in OC. With this first proof of concept experiment, we propose to call this promising field ‘bioelectrochemical nitrogen fixation’ (e-BNF): a possible way to ‘power’ biological nitrogen fixation, organic carbon storage and soil fertility against desertification, and possibly a new tool to study the development of early prokaryotic life in extreme environments.

    更新日期:2018-10-17
  • 更新日期:2018-10-11
  • Label-free independent quantitation of viable and non-viable cells using a multivariable multi-resonant sensor
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-10-10
    Radislav A. Potyrailo, Jon Dieringer, Victoria Cotero, Yongjae Lee, Steve Go, Matthew Schulmerich, Gunnar Malmquist, Andreas Castan, Klaus Gebauer, Vincent Pizzi

    Biological cells are utilized for diverse biotechnological and bioengineering purposes ranging from the production of biopharmaceuticals, to cell therapy, “human-on-a-chip” drug and toxicology assays, and drug-resistance tests. In these and other applications, it is critical to quantify the levels of not only viable but also non-viable cells. While traditional off-line cell-staining methods are available for counting of non-viable cells, many applications cannot periodically remove cells for their off-line analysis because of the risk of contamination or workflow logistics. Here we show in-situ label-free quantitation of viable and non-viable cells with multivariable multi-resonant sensors. We used Chinese hamster ovary (CHO) cells in suspension culture in single-use bioreactors as a representative example. The resonant sensor design strategy permitted enhanced sensor sensitivity versus conventional non-resonant measurements and probed the spectral dispersion of viable and non-viable cells with multiple resonances. These capabilities of label-free in-situ analysis of cell viability can be attractive in diverse cell applications such as cell suspensions, adhered cells, and their 3D assemblages.

    更新日期:2018-10-11
  • Molecular and histological study on the effects of electrolytic electroporation on the liver
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-09-30
    Yanpeng Lv, Yanfang Zhang, Boris Rubinsky

    This study examined the temporal physiological and molecular events following the treatment of the liver with a tissue ablation modality that combined electroporation with electrolysis (E2). Rat liver was treated with an E2 waveform and the tissue examined, 1 h, 3 h, 6 h and 24 h with: H&E, Masson Trichrome, TUNEL stains and Western blot. H&E and TUNEL stains have shown that cell death began to be evident 3 h and hepatocyte regeneration was seen 24 h after treatment. H&E and Masson trichrome have shown that the extracellular matrix and the large lumens, appeared intact after E2. Western blot has shown the following molecular events after E2: cleaved caspase 3–downgraded at 1 h, upgraded at 24 h (apoptosis); cleaved Caspase 1 and cleaved GSDMD–upgraded at 6 h (pyroptosis), RIP3–upgraded at 1 h, MLKL–upgraded at 3 h (necroptosis). The mechanism of cell death was possible initiated by necroptosis pathway. Pyroptosis pathway was also activated. The observation that cell death from E2 was by programed necrosis and the details on the temporal molecular pathways, may have value for the recent attempt to combine electroporation mediated ablation with immunological treatment, by demonstrating that the cell death from E2 involves an inflammatory response and by providing data that could be used to design the optimal timing for the injection of immunological adjuvants.

    更新日期:2018-10-01
  • The efficiency of heart protection with HTK or HTK-N depending on the type of ischemia
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-09-20
    Michael Schaefer, Martha-Maria Gebhard, Wolfgang Gross

    We investigated isolated guinea pig hearts (n = 121) in an ischemia/ reperfusion model with the aim to compare the efficiency of the cardioplegic solution HTK with its novel replacement HTKN. Following consolidation with Tyrode's solution, ischemia started either immediately or after preceding cardioplegia with HTK, HTKN, or modified HTK enriched with Ca. Ischemia lasted either 80 min at 30 °C, or 360 min at 5 °C, or 81 min at 30 °C with intermittent cardioplegic perfusion. During ischemia we measured intracellular calcium (iCa++) and the time of gap junction uncoupling (t-in). During reperfusion we measured the reestablishment of cell coupling (t-ret), left ventricular developed pressure (LVDP), and heart rhythm (VC-RR). In 5 °C groups, iCa++ at t-in was significantly higher than before ischemia, and longest t-in, shortest t-ret, and best VC-RR were observed after HTK-protection. Of all 30 °C groups, the intermittent group with modified HTK showed shortest t-ret, best VC-RR, and the highest LVDP. At 5 °C, HTK groups had higher LVDP than HTK-N groups, but not at 30 °C. The data suggest that the higher calcium level in the HTK-N solution improves reperfusion after short ischemia at 30 °C but for long lasting ischemia at 5 °C it is beneficial to use the HTK solution.

    更新日期:2018-09-21
  • Effect of external resistance on the sensitivity of microbial fuel cell biosensor for detection of different types of pollutants
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-09-17
    Yue Yi, Beizhen Xie, Ting Zhao, Zhaoming Li, Devard Stom, Hong Liu
    更新日期:2018-09-18
  • Low-cost nanowired α-MnO2/C as an ORR catalyst in air-cathode microbial fuel cell
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-09-15
    Mir Reza Majidi, Fatemeh Shahbazi Farahani, Mirghasem Hosseini, Iraj Ahadzadeh

    In this work, low cost α-MnO2 nanowires and α-MnO2 nanowires supported on carbon Vulcan (α-MnO2/C) have been synthesized via a simple and facile hydrothermal method for application in microbial fuel cells. The prepared samples have been characterized by X-ray diffraction (XRD), Raman spectroscopy and field emission scanning electron microscopy (FE-SEM). Electrocatalytic activities of the samples have been evaluated by means of cyclic voltammetry (CV), linear sweep voltammetry (LSV) and electrochemical impedance spectroscopy (EIS) in a neutral phosphate buffer solution. EIS was performed at different potentials to gain further insight into the kinetic properties of α-MnO2/C. Both catalysts were used in air cathode microbial fuel cells to achieve power densities of 180 and 111 mWm−2 for α-MnO2/C and pristine α-MnO2 nanowires, respectively. α-MnO2/C functions as a good and economical alternative for Pt free catalysts in practical MFC applications, as shown by the findings of stability test and voltage generation cycles in long-term operation of MFC.

    更新日期:2018-09-17
  • A novel bioelectrochemical method for real-time nitrate monitoring
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-09-15
    Shi-gang Su, Hao-yi Cheng, Ting-ting Zhu, Hong-cheng Wang, Ai-jie Wang

    Nitrate is one of the most common pollutants in the water environment. A key factor for the effective control and removal of nitrate is the ability to accurately determine the nitrate concentration in groundwater and the secondary effluent of wastewater treatment plants. Here, a bioelectrochemical method for real-time detection of the nitrate was developed. In this work, a kinetic model was developed to describe the correlation between the nitrate concentration and the current. Standard addition experiments showed the relative error between indicator predictions and ion chromatographic values ranged from 3.14% to 9.74%. The monitoring results of secondary effluent showed that the system could give a good response at different nitrate concentrations. The average error of not >10.85% between the indicator predictions and ion chromatographic values was demonstrated. This study offers a new method for the development of sustainable bioelectrochemical system (BES)-based technology for the real-time detection of nitrate in groundwater and the secondary effluent.

    更新日期:2018-09-17
  • 更新日期:2018-09-07
  • Cell-seeded 3D scaffolds as in vitro models for electroporation
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-08-30
    Paola Brun, Monica Dettin, Luca Giovanni Campana, Fabrizio Dughiero, Paolo Sgarbossa, Clara Bernardello, Anna Lisa Tosi, Annj Zamuner, Elisabetta Sieni

    Electroporation of cells is usually studied using cell suspensions or monolayer cultures. 3D scaffolds for cell culture have been recently designed in order to reproduce in vitro the complex and multifactorial environment experimented in vivo by cells. In fact, it is well known that 2D cell cultures are not able to simulate the complex interactions between the cells and their extracellular matrix (ECM). Recently, some examples of 3D models, like spheroids, have been investigated also in the electroporation field. Spheroids have been proposed in electrochemotherapy (ECT) studies to mimic tumor in vivo conditions: they are easy-to-handle 3D models but their sensitivity to electric field pulses depends from their diameter and, more interestingly, despite being relevant for intercellular junctions, they are not so much so for cell-ECM interactions. In this work, we propose a 3D macroscopic myxoid matrix for cell culture that would mimic the in vivo environment of myxoid stroma tumors. The myxoid stroma consists of abundant basic substances with large amounts of glycosaminoglycans (hyaluronic acid) and proteoglycans, poor collagen fibers and no elastin content. In the proposed approach, tumor cells seeded on 3D scaffolds mimic of myxoid stroma can establish both cell-cell and cell-ECM 3D interactions. The MCF7 cells (human breast adenocarcinoma cell line) were seeded in complete culture medium. Cell cultures were incubated at 37 °C for either 24 h, 3 days or 7 day. Some samples were used to assess cell vitality using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) test and others for electroporation tests and for histopathological analysis. The electroporation has been verified by the fluorescent dye Propidium cellular uptake. The proposed myxoid stroma scaffold induces cell proliferation and shows fibrous structures produced by cells, the concentration of which increases with culture time. The proposed matrix will be used for further investigations as a new scaffold for cell culture. Tumor cells grown into these new scaffolds will be used to evaluate electroporation including the stroma effect.

    更新日期:2018-08-31
  • Effect of isoelectric point on cheese whey wastewater treatment using a microbial electrochemical system
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-08-28
    Batoul Mohebrad, Abbas Rezaee, Beheshteh Sohrabi

    In this study, a microbial electrochemical system (MES) was employed to investigate the effect of isoelectric point (IEP) on cheese whey wastewater treatment. The experiments were carried out in a bioreactor equipped with a semicircular carbon cloth and stainless steel electrodes as anode and cathode, respectively. The effects of IEP, whey protein concentration, electrical current, and time were studied. The IEP of the whey protein was determined at pH 5.9. The optimum electrical current was obtained at 6 mA for synthetic cheese whey wastewater. The results of rotary exponential doping showed that the third structure of proteins chenges to the second structure at the IEP. The highest protein removal (98%) was obtained at pH 6. The results showed that 76%, 83%, and 98% protein removal were achieved at 2, 4, and 8 h, respectively.

    更新日期:2018-08-30
  • 更新日期:2018-07-12
  • Nanosecond bipolar pulse generators for bioelectrics
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-04-26
    Shu Xiao, Chunrong Zhou, Enbo Yang, Sambasiva R. Rajulapati

    Biological effects caused by a nanosecond pulse, such as cell membrane permeabilization, peripheral nerve excitation and cell blebbing, can be reduced or cancelled by applying another pulse of reversed polarity. Depending on the degree of cancellation, the pulse interval of these two pulses can be as long as dozens of microseconds. The cancellation effect diminishes as the pulse duration increases. To study the cancellation effect and potentially utilize it in electrotherapy, nanosecond bipolar pulse generators must be made available. An overview of the generators is given in this paper. A pulse forming line (PFL) that is matched at one end and shorted at the other end allows a bipolar pulse to be produced, but no delay can be inserted between the phases. Another generator employs a combination of a resistor, an inductor and a capacitor to form an RLC resonant circuit so that a bipolar pulse with a decaying magnitude can be generated. A third generator is a converter, which converts an existing unipolar pulse to a bipolar pulse. This is done by inserting an inductor in a transmission line. The first phase of the bipolar pulse is provided by the unipolar pulse's rising phase. The second phase is formed during the fall time of the unipolar pulse, when the inductor, which was previously charged during the flat part of the unipolar pulse, discharges its current to the load. The fourth type of generator uses multiple MOSFET switches stacked to turn on a pre-charged, bipolar RC network. This approach is the most flexible in that it can generate multiphasic pulses that have different amplitudes, delays, and durations. However, it may not be suitable for producing short nanosecond pulses (<100 ns), whereas the PFL approach and the RLC approach with gas switches are used for this range. Thus, each generator has its own advantages and applicable range.

    更新日期:2018-07-12
  • An electrochemical sensing approach for scouting microbial chemolithotrophic metabolisms
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-05-01
    Albert Saavedra, Federico Figueredo, Eduardo Cortón, Ximena C. Abrevaya
    更新日期:2018-07-12
  • Binder materials for the cathodes applied to self-stratifying membraneless microbial fuel cell
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-04-19
    Xavier Alexis Walter, John Greenman, Ioannis Ieropoulos

    The recently developed self-stratifying membraneless microbial fuel cell (SSM-MFC) has been shown as a promising concept for urine treatment. The first prototypes employed cathodes made of activated carbon (AC) and polytetrafluoroethylene (PTFE) mixture. Here, we explored the possibility to substitute PTFE with either polyvinyl-alcohol (PVA) or PlastiDip (CPD; i.e. synthetic rubber) as binder for AC-based cathode in SSM-MFC. Sintered activated carbon (SAC) was also tested due to its ease of manufacturing and the fact that no stainless steel collector is needed. Results indicate that the SSM-MFC having PTFE cathodes were the most powerful measuring 1617 μW (11 W·m−3 or 101 mW·m−2). SSM-MFC with PVA and CPD as binders were producing on average the same level of power (1226 ± 90 μW), which was 24% less than the SSM-MFC having PTFE-based cathodes. When balancing the power by the cost and environmental impact, results clearly show that PVA was the best alternative. Power wise, the SAC cathodes were shown being the less performing (≈1070 μW). Nonetheless, the lower power of SAC was balanced by its inexpensiveness. Overall results indicate that (i) PTFE is yet the best binder to employ, and (ii) SAC and PVA-based cathodes are promising alternatives that would benefit from further improvements.

    更新日期:2018-07-12
  • Electrochemical behavior of pyrite in sulfuric acid in presence of amino acids belonging to the amino acid sequence of rusticyanin
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-05-01
    Biljana S. Maluckov, Miodrag N. Mitrić

    The impact of different concentrations of three amino acids (cysteine, histidine and methionine) which are part of the amino acid sequence of rusticyanin on dissolution of pyrite is investigated by the application of electrochemical techniques. Cyclic voltammetric studies conducted in the anodic direction from corrosion potential have shown that in the vicinity of corrosion potential, histidine and methionine do not influence dissolution of pyrite independently on their concentrations. On the other hand, cysteine and solutions of these amino acids in the molar ratios Cys:His:Met/1:1:1 and Cys:His:Met/1:2:1 accelerate dissolution at concentrations 10−2 mol L−1 and 10−3 mol L−1. Potentiodynamic polarization measurements showed that methionine does not affect the anodic and cathodic dissolution at all concentrations, while histidine does not affect significantly on the anodic dissolution at all concentrations. Cysteine and solutions of three amino acids in the molar ratio Cys:His:Met/1:1:1 and Cys:His:Met/1:2:1 cause intensive cathodic inhibition and anodic activation at concentrations 10−2 mol L−1 and 10−3 mol L−1 respectively.

    更新日期:2018-07-12
  • 更新日期:2018-07-12
  • Hydrogen peroxide biosensor based on chitosan/2D layered double hydroxide composite for the determination of H2O2
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-04-23
    Jian Yuan, Sheng Xu, Hong-Yan Zeng, Xi Cao, A. Dan Pan, Gao-Fei Xiao, Peng-Xuan Ding

    The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV–vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy. The use of CMC could impact the textural and surface chemical properties of the LDH-CMC composites, where the composites still maintained the 2D layered structure. Incorporating a moderate amount of CMC could increase both the surface area and the permanent charge density of the composites, leading to improved electrochemical performances. The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area. The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02–6.0 mmol·L−1 with a low detection limit of 12.4 μmol·L−1, good anti-interference ability and long-term storage stability. The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.

    更新日期:2018-07-12
  • Severe microbiologically influenced corrosion of S32654 super austenitic stainless steel by acid producing bacterium Acidithiobacillus caldus SM-1
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-04-23
    Yuqiao Dong, Baota Jiang, Dake Xu, Chengying Jiang, Qi Li, Tingyue Gu

    Microbiologically influenced corrosion (MIC) of S32654 (654SMO) super austenitic stainless steel (SASS) by acid producing bacterium (APB), Acidithiobacillus caldus SM-1, a strain of sulfur-oxidizing bacteria (SOB) used in biohydrometallurgy field, was investigated using electrochemical measurements and surface characterizations during a 14-day immersion test. The results indicated that S32654 SASS was susceptible to MIC by APB, and A. caldus SM-1 was capable of producing an aggressive acidic environment underneath the biofilm, resulting in the dissolution of the passive film and severe pitting attacks against S32654 SASS, which is commonly regarded as a corrosion resistant material.

    更新日期:2018-07-12
  • A novel drug-free strategy of nano-pulse stimulation sequence (NPSS) in oral cancer therapy: In vitro and in vivo study
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-04-19
    Jinsong Guo, Feihong Dong, Lian Ding, Kaile Wang, Jue Zhang, Jing Fang

    Nano-pulse stimulation (NPS) is a novel technology to induce cancer apoptosis. In this study, based on the energy-dose effect of NPS, we designed a special NPS sequence (NPSS) with low field intensity. The effectiveness and mechanisms of NPSS on oral cancer therapy were evaluated by cell proliferation assays, microscopic investigation, JC-1 mitochondrial membrane potential assay, tumor inhibition assays, immunohistochemistry (IHC) assay, Ca2+, NOS and ROS detection assays, respectively. The results demonstrated that NPSS treatment significantly inhibited oral cancer growth in vitro and in vivo. Furthermore, we found that NPSS treatment induced an obviously apoptosis and mitochondrial membrane potential (ΔΨm) reduction in Cal-27 cells. Notably, further experiments revealed that the mechanisms of crosstalk signaling between NO, ROS and Ca2+ involvement in NPSS treatment. In conclusion, this is a proof-of-concept study that provides a potential alternative strategy for developing and applying NPSS in oral cancer therapy.

    更新日期:2018-07-12
  • Investigation of ceramic MFC stacks for urine energy extraction
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-03-21
    Asimina Tremouli, John Greenman, Ioannis Ieropoulos

    Two ceramic stacks, terracotta (t-stack) and mullite (m-stack), were developed to produce energy when fed with neat undiluted urine. Each stack consisted of twelve identical microbial fuel cells (MFCs) which were arranged in cascades and tested under different electrical configurations. Despite voltage reversal, the m-stack produced a maximum power of 800 μW whereas the t-stack produced a maximum of 520 μW after 62.6 h of operation. Moreover, during the operation, both systems were subject to blockage possibly due to struvite. To the Authors' best knowledge, this is the first time that such a phenomenon in ceramic MFC membranes is shown to be the direct result of sub-optimal performance, which confirms the hypothesis that ceramic membranes can continue operating long-term, if the MFCs produce maximum power (high rate of e− transfer). Furthermore, it is shown that once the ceramic membrane is blocked, it may prove difficult to recover in-situ.

    更新日期:2018-07-12
  • Biological effects in photodynamic treatment combined with electropermeabilization in wild and drug resistant breast cancer cells
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-04-19
    Joanna Weżgowiec, Julita Kulbacka, Jolanta Saczko, Joanna Rossowska, Grzegorz Chodaczek, Małgorzata Kotulska

    Electrochemotherapy became one of the therapeutic protocols successfully used in oncology. However, biological effects occurring in cells, especially those which are drug resistant, have not been studied thoroughly. This study presents response of wild and drug resistant breast cancer cells to classical photodynamic therapy with Photofrin or experimental photodynamic therapy with cyanine IR-775, combined with electroporation.Photodynamic reaction or electroporation alone had no cytotoxic effect, but their combination significantly disturbed cellular functions. Applying electroporation allowed the drugs to increase its accumulation, especially for a poorly permeant cyanine in drug resistant cells. FACS analysis showed that even at relatively mild electric field, ca. 90% of cells were permeabilized. High intracellular concentration of drugs triggered the cellular defense system through increased expression of glutathione S-transferase and multidrug resistance proteins (MDR1 and MRP7), particularly in drug resistant cells. Finally, expressively decreased cell metabolism and proliferation, as well as formation of apoptotic bodies and fragmentation of cells were observed after the combined treatment.The results show that electroporation can be used for effective delivery of photosensitizers, even to drug resistant breast cancer cells, which was not tested before. This shows that electro-photodynamic treatment could be a promising approach to overcome a problem of drug resistance in cancer cells.

    更新日期:2018-07-12
  • On the interaction of the highly charged peptides casocidins with biomimetic membranes
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-04-19
    Lucia Becucci, Giovanni Aloisi, Andrea Scaloni, Simonetta Caira, Rolando Guidelli

    Casocidin I and II (CI and CII) are structurally related antimicrobial peptides made of 39 and 31 amino acids, respectively, which derive from natural proteolytic processing of αs2-casein and adopt an ordered α-helical structure in biomimetic membranes. Their putative membrane-permeabilizing activity was investigated at Hg-supported self-assembled monolayers (SAMs) and at tethered bilayer lipid membranes (tBLMs); the latter consisted of a monolayer of 2,3,di-O-phytanyl-sn-glycerol-1-tetraethylene-glycol-d,l-α lipoic acid ester thiolipid (DPTL), with a dioleoylphosphatidylcholine (DOPC) or dioleoylphosphatidylserine (DOPS) monolayer on top of it. Interaction of CI and CII with these biomimetic membranes was studied by four electrochemical techniques at pH 3, 5.4 and 6.8. Peptide incorporation in tBLMs was attempted via scans of electrochemical impedance spectra. Experiments demonstrated that CI and CII penetrate SAMs as well as the distal DOPC monolayer of DPTL/DOPC tBLMs, but not the proximal phytanyl monolayer, with the only exception of CII at pH 5.4. Conversely, CII permeabilized DPTL/DOPS tBLMs to a moderate extent at all investigated pH values by forming holes across the membrane, but not ion channels. Structural distribution of charged residues seemed to prevent CII from having a hydrophobic side of the α-helix capable of stabilizing a regular ion channel in the lipid matrix.

    更新日期:2018-07-12
  • Bioelectrical coupling in multicellular domains regulated by gap junctions: A conceptual approach
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-04-21
    Javier Cervera, Alexis Pietak, Michael Levin, Salvador Mafe
    更新日期:2018-07-12
  • Design and fabrication of an electrochemical aptasensor using Au nanoparticles/carbon nanoparticles/cellulose nanofibers nanocomposite for rapid and sensitive detection of Staphylococcus aureus
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-04-27
    Saba Ranjbar, Saeed Shahrokhian

    Since that pathogenic bacteria are major threats to human health, this paper describes the fabrication of an effective and durable sensing platform based on gold nanoparticles/carbon nanoparticles/cellulose nanofibers nanocomposite (AuNPs/CNPs/CNFs) at the surface of glassy carbon electrode for sensitive and selective detection of Staphylococcus aureus (S. aureus). The AuNPs/CNPs/CNFs nanocomposite with the high surface area, excellent conductivity, and good biocompatibility was used for self-assembled of the thiolated specific S. aureus aptamer as a sensing element. The surface morphology of AuNPs/CNPs/CNFs nanocomposite was characterized with field emission scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), dynamic light scattering (DLS) and ultraviolet-visible (UV–Vis) spectrophotometric methods. Each aptasensor modification step was monitored with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. The fabricated aptasensor exhibited a wide linear dynamic range (1.2 × 101 to 1.2 × 108) CFU mL−1 with a LOD of 1 CFU mL−1 and was be capable to accurate detection and determination of Staphylococcus aureus in human blood serum as a clinical sample with a complex matrix.

    更新日期:2018-05-03
  • Growth and current production of mixed culture anodic biofilms remain unaffected by sub-microscale surface roughness
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-04-12
    Melanie Pierra, Mehdi Golozar, Xu Zhang, Antonin Prévoteau, Michael de Volder, Dominiek Reynaerts, Korneel Rabaey

    Bioelectrochemical systems couple electricity demand/supply to the metabolic redox reactions of microorganisms. Generally, electrodes act not only as electron acceptors/donors, but also as physical support for an electroactive biofilm. The microorganism-electrode interface can be modified by changing the chemical and/or topographical features of the electrode surface. Thus far, studies have reported conflicting results on the impact of the electrode surface roughness on the growth and current production of biofilms. Here, the surface roughness of the glassy carbon electrodes was successfully modified at the sub-microscale using micro electrodischarge machining, while preserving the surface chemistry of the parent glassy carbon. All microbial electrodes showed similar startup time, maximum current density, charge transport ability across the biofilm and biomass production. Interestingly, an increase in the average surface cavity depth was observed for the biofilm top layer as a function of the electrode surface roughness (from 7 μm to 16 μm for a surface roughness of 5 nm to 682 nm, respectively). These results indicated that the surface roughness at a sub-microscale does not significantly impact the attachment or current production of mixed culture anodic biofilms on glassy carbon. Likely earlier observations were associated with changes in surface chemistry, rather than surface topography.

    更新日期:2018-04-17
  • Different permeabilization patterns of splenocytes and thymocytes to combination of pulsed electric and magnetic field treatments
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-04-10
    Vitalij Novickij, Auksė Zinkevičienė, Justinas Valiulis, Jurgita Švedienė, Algimantas Paškevičius, Eglė Lastauskienė, Svetlana Markovskaja, Jurij Novickij, Irutė Girkontaitė
    更新日期:2018-04-11
  • Engineered fungus derived FAD-dependent glucose dehydrogenase with acquired ability to utilize hexaammineruthenium(III) as an electron acceptor
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-04-10
    Madoka Okurita, Nanami Suzuki, Noya Loew, Hiromi Yoshida, Wakako Tsugawa, Kazushige Mori, Katsuhiro Kojima, David C. Klonoff, Koji Sode

    Fungal FAD-dependent glucose dehydrogenases (FADGDHs) are considered to be superior enzymes for glucose sensor strips because of their insensitivity to oxygen and maltose. One highly desirable mediator for enzyme sensor strips is hexaammineruthenium(III) chloride because of its low redox potential and high storage stability. However, in contrast to glucose oxidase (GOx), fungal FADGDH cannot utilize hexaammineruthenium(III) as electron acceptor. Based on strategic structure comparison between FADGDH and GOx, we constructed a mutant of Aspergillus flavus-derived FADGDH, capable of utilizing hexaammineruthenium(III) as electron acceptor: AfGDH-H403D. In AfGDH-H403D, a negative charge introduced at the pathway-entrance leading to the FAD attracts the positively charged hexaammineruthenium(III) and guides it into the pathway. The corresponding amino acid in wild-type GOx is negatively charged, which explains the ability of GOx to utilize hexaammineruthenium(III) as electron acceptor. Electrochemical measurements showed a response current of 46.0 μA for 10 mM glucose with AfGDH-H403D and hexaammineruthenium(III), similar to that with wild-type AfGDH and ferricyanide (47.8 μA). Therefore, AfGDH-H403D is suitable for constructing enzyme electrode strips with hexaammineruthenium(III) chloride as sole mediator. Utilization of this new, improved fungal FADGDH should lead to the development of sensor strips for blood glucose monitoring with increased accuracy and less stringent packing requirements.

    更新日期:2018-04-11
  • Development of an adaptive electroporation system for intratumoral plasmid DNA delivery
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-04-09
    Douglas W. Brown, Arya J. Bahrami, David A. Canton, Anandaroop Mukhopadhyay, Jean S. Campbell, Robert H. Pierce, Richard J. Connolly

    Intratumoral electroporation of plasmid DNA encoding the proinflammatory cytokine interleukin 12 promotes innate and adaptive immune responses correlating with anti-tumor effects. Clinical electroporation conditions are fixed parameters optimized in preclinical tumors, which consist of cells implanted into skin. These conditions have little translatability to clinically relevant tumors, as implanted models cannot capture the heterogeneity encountered in genetically engineered mouse models or clinical tumors. Variables affecting treatment outcome include tumor size, degree of vascularization, fibrosis, and necrosis, which can result in suboptimal gene transfer and variable therapeutic outcomes. To address this, a feedback controlled electroporation generator was developed, which is capable of assessing the electrochemical properties of tissue in real time. Determination of these properties is accomplished by impedance spectroscopy and equivalent circuit model parameter estimation. Model parameters that estimate electrical properties of cell membranes are used to adjust electroporation parameters for each applied pulse. Studies performed in syngeneic colon carcinoma tumors (MC38) and spontaneous mammary tumors (MMTV-PyVT) demonstrated feedback-based electroporation is capable of achieving maximum expression of reporter genes with significantly less variability and applied energy. These findings represent an advancement to the practice of gene electro-transfer, as reducing variability and retaining transfected cell viability is paramount to treatment success.

    更新日期:2018-04-10
  • A highly sensitive electrochemical biosensor for phenol derivatives using a graphene oxide-modified tyrosinase electrode
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-04-09
    Yue Wang, Fengge Zhai, Yasushi Hasebe, Hongmin Jia, Zhiqiang Zhang

    The fabrication, characterization and analytical performance were investigated for a phenol biosensor based on the covalent bonding of tyrosinase (TYR) onto a graphene oxide (GO)-modified glassy carbon electrode (GCE) via glutaraldehyde (GA). The surface morphology of the modified electrode was studied by atomic force microscope (AFM) and field-emission scanning electron microscopy (FE-SEM). The fabricated TYR/GA/GO/GCE biosensor showed very good stability, reproducibility, sensitivity and practical usage. The catechol biosensor exhibited a wide sensing linear range from 5 × 10−8 M to 5 × 10−5 M, a lower detection limit of 3 × 10−8 M, a current maximum (Imax) of 65.8 μA and an apparent Michaelis constant (Kmapp) of 169.9 μM.

    更新日期:2018-04-10
  • Gold nanoparticle-decorated reduced-graphene oxide targeting anti hepatitis B virus core antigen
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-04-09
    Mohamad Farid Abd Muain, Kooi Hoong Cheo, Muhamad Nadzmi Omar, Amir Syahir Amir Hamzah, Hong Ngee Lim, Abu Bakar Salleh, Wen Siang Tan, Asilah Ahmad Tajudin

    Hepatitis B virus core antigen (HBcAg) is the major structural protein of hepatitis B virus (HBV). The presence of anti-HBcAg antibody in a blood serum indicates that a person has been exposed to HBV. This study demonstrated that the immobilization of HBcAg onto the gold nanoparticles-decorated reduced graphene oxide (rGO-en-AuNPs) nanocomposite could be used as an antigen-functionalized surface to sense the presence of anti-HBcAg. The modified rGO-en-AuNPs/HBcAg was then allowed to undergo impedimetric detection of anti-HBcAg with anti-estradiol antibody and bovine serum albumin as the interferences. Upon successful detection of anti-HBcAg in spiked buffer samples, impedimetric detection of the antibody was then further carried out in spiked human serum samples. The electrochemical response showed a linear relationship between electron transfer resistance and the concentration of anti-HBcAg ranging from 3.91 ng mL−1 to 125.00 ng mL−1 with lowest limit of detection (LOD) of 3.80 ng mL−1 at 3 σ m−1. This established method exhibits potential as a fast and convenient way to detect anti-HBcAg.

    更新日期:2018-04-10
  • An impedimetric biosensor for DNA damage detection and study of the protective effect of deferoxamine against DNA damage
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-03-28
    Seyedeh Zeinab Mousavisani, Jahan Bakhsh Raoof, Reza Ojani, Zahra Bagheryan
    更新日期:2018-04-07
  • Controlled synthesis of mixed molecular nanostructures from folate and deferrioxamine-Ga(III) on gold and tuning their performance for cancer cells
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-03-28
    Reza Karimi Shervedani, Fatemeh Yaghoobi, Mostafa Torabi, Fatemeh Rahnemaye Rahsepar, Marzieh Samiei Foroushani
    更新日期:2018-04-07
  • The second phase of bipolar, nanosecond-range electric pulses determines the electroporation efficiency
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-03-29
    Andrei G. Pakhomov, Sergey Grigoryev, Iurii Semenov, Maura Casciola, Chunqi Jiang, Shu Xiao

    Bipolar cancellation refers to a phenomenon when applying a second electric pulse reduces (“cancels”) cell membrane damage by a preceding electric pulse of the opposite polarity. Bipolar cancellation is a reason why bipolar nanosecond electric pulses (nsEP) cause weaker electroporation than just a single unipolar phase of the same pulse. This study was undertaken to explore the dependence of bipolar cancellation on nsEP parameters, with emphasis on the amplitude ratio of two opposite polarity phases of a bipolar pulse. Individual cells (CHO, U937, or adult mouse ventricular cardiomyocytes (VCM)) were exposed to either uni- or bipolar trapezoidal nsEP, or to nanosecond electric field oscillations (NEFO). The membrane injury was evaluated by time-lapse confocal imaging of the uptake of propidium (Pr) or YO-PRO-1 (YP) dyes and by phosphatidylserine (PS) externalization. Within studied limits, bipolar cancellation showed little or no dependence on the electric field intensity, pulse repetition rate, chosen endpoint, or cell type. However, cancellation could increase for larger pulse numbers and/or for longer pulses. The sole most critical parameter which determines bipolar cancellation was the phase ratio: maximum cancellation was observed with the 2nd phase of about 50% of the first one, whereas a larger 2nd phase could add a damaging effect of its own. “Swapping” the two phases, i.e., delivering the smaller phase before the larger one, reduced or eliminated cancellation. These findings are discussed in the context of hypothetical mechanisms of bipolar cancellation and electroporation by nsEP.

    更新日期:2018-04-06
  • Viability and membrane lipid composition under a 57 mT static magnetic field in Salmonella Hadar
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-03-29
    Ramla Ben Mouhoub, Alya El May, Imen Boujezza, Mohamed Marouen Sethom, Moncef Feki, Ahmed Landoulsi

    The aim of this work is to demonstrate the effects of a static magnetic field (SMF) with an induction 12 equal to 57 mT on the viability and membrane lipid composition of Salmonella Hadar. Results showed an increase in the viability of exposed bacteria compared to controls after 9 h of exposure. Analysis with gas chromatography of total lipids (TLs) and different fractions of phospholipids: phosphatidylglycerols (PGs), phosphatidylethanolamines (PEs), and cardiolipins (CLs), separated by thin layer chromatography revealed changes in fatty acid levels during exposure. For TLs, the unsaturated fatty acids/saturated fatty acids ratio (UFAs/SFAs) had significantly increased after 9 h of exposure. The variation of this ratio seems to be essentially due to the increase of the proportion of unsaturated fatty acids with 18 carbons, in particular C18:1. The analyses of fatty acid composition carried out on the scale of each fraction of phospholipids showed that CLs contributed significantly to the increase of the proportion of the unsaturated fatty acids between 6 and 9 h of exposure thanks to their unsaturated chains with 18 carbons (especially C18:2). CLs appear to be the main phospholipid involved in the adaptation of S. Hadar membranes to the SMF.

    更新日期:2018-04-06
  • An insoluble iron complex coated cathode enhances direct electron uptake by Rhodopseudomonas palustris TIE-1
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-04-05
    Karthikeyan Rengasamy, Tahina Ranaivoarisoa, Rajesh Singh, Arpita Bose

    Microbial electrosynthesis (MES) is a promising bioelectrochemical approach to produce biochemicals. A previous study showed that Rhodopseudomonas palustris TIE-1 can directly use poised electrodes as electron donors for photoautotrophic growth at cathodic potentials that avoid electrolytic H2 production (photoelectroautotrophy). To make TIE-1 an effective biocatalyst for MES, we need to improve its electron uptake ability and growth under photoelectroautotrophic conditions. Because TIE-1 interacts with various forms of iron while using it as a source of electrons for photoautotrophy (photoferroautotrophy), we tested the ability of iron-based redox mediators to enhance direct electron uptake. Our data show that soluble iron cannot act as a redox mediator for electron uptake by TIE-1 from a cathode poised at +100 mV vs. Standard Hydrogen electrode. We then tested whether an immobilized iron-based redox mediator Prussian blue (PB) can enhance electron uptake by TIE-1. Chronoamperometry indicates that cathodic current uptake by TIE-1 increased from 1.47 ± 0.04 to 5.6 ± 0.09 μA/cm2 (3.8 times). Overall, our data show that immobilized PB can enhance direct electron uptake by TIE-1.

    更新日期:2018-04-06
  • Proteus vulgaris - Pt electrode system for urea to nitrogen conversion in synthetic urine
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-04-04
    Myreisa Morales-Cruz, Marjorie López-Nieves, Roberto Morales-Hernández, Gian C. Rivera-Crespo, Gary A. Toranzos, Ileana González-González, Carlos R. Cabrera

    One of the most challenging problems when trying to recycle urine for different purposes is the removal of urea. In this project we studied an ureolysis system using the bacterium Proteus vulgaris for the transformation of urea to ammonia and its subsequent oxidation to nitrogen at a Pt working electrode. Our system was tested under different pH, microbial reaction times, and urea and bacteria concentrations. Our results indicate that a pH 8 is optimal for the combined Proteus vulgaris urease activity and the ammonia oxidation reaction at a Pt electrode. The reaction time and concentration dependence on the ammonia oxidation reaction current densities was also studied. Results showed limited ammonia oxidation under high urea concentrations in ~2.5 × 109 cfu/mL Proteus vulgaris in synthetic urine.

    更新日期:2018-04-06
Some contents have been Reproduced with permission of the American Chemical Society.
Some contents have been Reproduced by permission of The Royal Society of Chemistry.
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华中师范大学化学生物学中心招聘化学类博士后
哈尔滨工业大学刘绍琴教授课题组诚招博士后、科研助理
南方科技大学讲座教授郑智平团队——行政秘书招聘启事
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