显示样式:     当前期刊: Bioelectrochemistry    加入关注       排序: 导出
我的关注
我的收藏
您暂时未登录!
登录
  • Exfoliated molybdenum di-sulfide (MoS2) electrode for hydrogen production in microbial electrolysis cell
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-05-19
    Shmuel Rozenfeld, Hanan Teller, Michal Schechter, Ravit Farber, Olga Krichevski, Alex Schechter, Rivka Cahan

    The most widely reported catalyst in microbial electrochemical cells (MEC) cathodes is platinum (Pt). The disadvantages of Pt include its high cost and sensitivity to various molecules. In this research an exfoliated molybdenum di-sulfide (MoS2-EF) catalyst was synthesized. The size of the obtained particles was 200 ± 50 nm, 50-fold smaller than the pristine MoS2 catalyst. The MoS2-EF Raman spectrum displays the E12g and A1g peaks at 373 cm−1 and 399 cm−1. Electrochemical characterization by linear sweep voltammetry (LSV) of a rotating disc electrode RDE showed that the current density of Pt in 0.5 M H2SO4 was 3.3 times higher than MoS2-EF. However, in phosphate buffer (pH-7) electrolyte this ratio diminished to 1.9. The polarization curve of Pt, MoS2-EF and the pristine MoS2 electrodes, at −1.3 V in MEC configuration in abiotic conditions exhibit current densities of 17.46, 12.67 and 3.09 mA cm−2, respectively. Hydrogen evolution rates in the same MEC with a Geobacter sulfurreducens anode and Pt, MoS2-EF and the pristine MoS2 cathodes were 0.106, 0.133 and 0.083 m3 d−1 m−3, respectively. The results in this study show that MoS2-EF led to highly purified hydrogen and that this catalyst can serve as an electrochemical active and cost-effective alternative to Pt.

    更新日期:2018-05-27
  • Nanomaterials for facilitating microbial extracellular electron transfer: Recent progress and challenges
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-05-05
    Peng Zhang, Jia Liu, Youpeng Qu, Da Li, Weihua He, Yujie Feng
    更新日期:2018-05-24
  • Interaction of DNA and mononucleotides with theophylline investigated using electrochemical biosensors and biosensing
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-05-03
    Katarína Nemčeková, Ján Labuda, Viktor Milata, Jana Blaškovičová, Jozef Sochr
    更新日期:2018-05-22
  • Investigation of interactions of Comtan with human serum albumin by mathematically modeled voltammetric data: A study from bio-interaction to biosensing
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-05-10
    Ali R. Jalalvand, Sirous Ghobadi, Hector C. Goicoechea, Hui-Wen Gu, Esmael Sanchooli

    In this work, voltammetric data recorded at a glassy carbon electrode (GCE) were separately used to investigate the interactions of entacapone (Comtan, CAT) with human serum albumin (HSA). Then, an augmented data matrix was constructed by the combination of voltammetric and spectroscopic data and simultaneously analysed by multivariate curve resolution-alternating least squares (MCR-ALS) to obtain more information about CAT-HSA interactions. The absence of rotational ambiguities in results obtained by MCR-ALS was verified with the help of MCR-BANDS and we confirmed that the results were unambiguous and reliable. Binding of CAT to HSA was also modeled by molecular docking and the results were compatible with those of obtained by recording experimental data. Hard-modeling of combined voltammetric and spectroscopic data by EQUISPEC as an efficient chemometric algorithm helped us to compute binding constant of CAT-HSA complex specie which was in a good agreement with the binding constant value obtained by direct analysis of experimental data. For electrochemical sensing of serum albumin two amperometric measurements were performed to determine HSA in 2–27 nM and 27–70 nM with a limit of detection of 0.51 nM and a sensitivity of 1.84 μA nM−1.

    更新日期:2018-05-17
  • Electrodeposited styrylquinolinium dye as molecular electrocatalyst for coupled redox reactions
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-05-10
    Yolina Hubenova, Rumyana Bakalska, Mario Mitov
    更新日期:2018-05-17
  • Electrotrophic activity and electrosynthetic acetate production by Desulfobacterium autotrophicum HRM2
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-05-04
    Zehra Zaybak, Bruce E. Logan, John M. Pisciotta

    Electroautotrophic microorganisms accept electrons from a cathode as source of reducing equivalents to drive CO2 fixation by poorly understood mechanisms. Acetogenic bacteria were the first group found to possess the capability for electroautotrophic metabolism in pure culture with associated electrosynthesis of acetate as primary metabolite. Identification of additional electrotrophic species can contribute to our understanding of this unusual form of metabolism. Here, bioelectrochemical techniques, chemical analysis and microscopy were used to determine electrotrophic metabolism of Desulfobacterium autotrophicum HRM2. Chronoamperometry showed increasing current uptake over 21 days of incubation in duplicate bioelectrochemical system sets. Linear sweep voltammetry indicated peak current uptake at −243 mV. High performance liquid chromatography (HPLC) analysis quantified acetate accumulation in anaerobic minimal media containing inorganic carbon as sole carbon source, consistent with electrosynthesis. Scanning electron microscopy and live/dead staining by epifluorescence microscopy analysis indicated viable 1–2 μm cells after 76 days of cultivation under electroautotrophic conditions. The genome of Db. autotrophicum HRM2 is fully sequenced and, thus, could provide insight into the biochemical and physiological mechanisms by which electrotrophic cells utilize cathode-derived electrons. This research expands the diversity of facultative autotrophs capable of electrotrophic metabolism to include the sulfate-reducing marine bacterium Db. autotrophicum HRM2.

    更新日期:2018-05-11
  • Electricity generation and microbial communities in microbial fuel cell powered by macroalgal biomass
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-05-05
    Nannan Zhao, Yinan Jiang, Merlin Alvarado-Morales, Laura Treu, Irini Angelidaki, Yifeng Zhang
    更新日期:2018-05-11
  • Reactivation of standard [NiFe]-hydrogenase and bioelectrochemical catalysis of proton reduction and hydrogen oxidation in a mediated-electron-transfer system
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-05-05
    Saeko Shiraiwa, Keisei So, Yu Sugimoto, Yuki Kitazumi, Osamu Shirai, Koji Nishikawa, Yoshiki Higuchi, Kenji Kano
    更新日期:2018-05-11
  • The anti-biofouling behavior of high voltage pulse electric field (HPEF) mediated by carbon fiber composite coating in seawater
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-25
    Tiantian Feng, Jinyi Wu, Ke Chai, Pengpeng Yang

    One of the most important research areas in the marine industry is to investigate new and effective anti-biofouling technologies. In this study, high voltage pulse electric field (HPEF) mediated by carbon fiber (CF) composite coating was utilized to prevent the fouling of bacteria, microalgae and barnacle larvae in seawater. The plate count, 2, 3, 5-triphenyl-tetrazolium chloride (TTC) reduction assay and neutral red (NR) staining and larval motility detection showed that the inactivation rates were at the highest levels, which reached 99.1%, 99.9%, 99.7%, 98.7% and 85% respectively for Pseudomonas sp., Vibrio sp., iron bacteria, Navicula sp. and the second stage nauplii of Balanus reticulatus, under the HPEF with 19 kV pulse amplitude, 23.15 kHz frequency and 0.5 duty cycle. The field-emission scanning electron microscopy (FE-SEM) of Navicula sp. revealed that the HPEF brought about the cell lysis and the cell organic matter release on the coating, which could be the mechanism of the inactivation by the HPEF. Additionally, the FE-SEM and Raman spectroscopy indicated that the HPEF hardly damaged the coating.

    更新日期:2018-05-09
  • Electrochemical behavior of pyrite in sulfuric acid in presence of amino acids belonging to the amino acid sequence of rusticyanin
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-05-01
    Biljana S. Maluckov, Miodrag N. Mitrić

    The impact of different concentrations of three amino acids (cysteine, histidine and methionine) which are part of the amino acid sequence of rusticyanin on dissolution of pyrite is investigated by the application of electrochemical techniques. Cyclic voltammetric studies conducted in the anodic direction from corrosion potential have shown that in the vicinity of corrosion potential, histidine and methionine do not influence dissolution of pyrite independently on their concentrations. On the other hand, cysteine and solutions of these amino acids in the molar ratios Cys:His:Met/1:1:1 and Cys:His:Met/1:2:1 accelerate dissolution at concentrations 10−2 mol L−1 and 10−3 mol L−1. Potentiodynamic polarization measurements showed that methionine does not affect the anodic and cathodic dissolution at all concentrations, while histidine does not affect significantly on the anodic dissolution at all concentrations. Cysteine and solutions of three amino acids in the molar ratio Cys:His:Met/1:1:1 and Cys:His:Met/1:2:1 cause intensive cathodic inhibition and anodic activation at concentrations 10−2 mol L−1 and 10−3 mol L−1 respectively.

    更新日期:2018-05-08
  • An electrochemical sensing approach for scouting microbial chemolithotrophic metabolisms
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-05-01
    Albert Saavedra, Federico Figueredo, Eduardo Cortón, Ximena C. Abrevaya
    更新日期:2018-05-08
  • Binder materials for the cathodes applied to self-stratifying membraneless microbial fuel cell
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-19
    Xavier Alexis Walter, John Greenman, Ioannis Ieropoulos

    The recently developed self-stratifying membraneless microbial fuel cell (SSM-MFC) has been shown as a promising concept for urine treatment. The first prototypes employed cathodes made of activated carbon (AC) and polytetrafluoroethylene (PTFE) mixture. Here, we explored the possibility to substitute PTFE with either polyvinyl-alcohol (PVA) or PlastiDip (CPD; i.e. synthetic rubber) as binder for AC-based cathode in SSM-MFC. Sintered activated carbon (SAC) was also tested due to its ease of manufacturing and the fact that no stainless steel collector is needed. Results indicate that the SSM-MFC having PTFE cathodes were the most powerful measuring 1617 μW (11 W·m−3 or 101 mW·m−2). SSM-MFC with PVA and CPD as binders were producing on average the same level of power (1226 ± 90 μW), which was 24% less than the SSM-MFC having PTFE-based cathodes. When balancing the power by the cost and environmental impact, results clearly show that PVA was the best alternative. Power wise, the SAC cathodes were shown being the less performing (≈1070 μW). Nonetheless, the lower power of SAC was balanced by its inexpensiveness. Overall results indicate that (i) PTFE is yet the best binder to employ, and (ii) SAC and PVA-based cathodes are promising alternatives that would benefit from further improvements.

    更新日期:2018-05-08
  • 更新日期:2018-05-07
  • Hydrogen peroxide biosensor based on chitosan/2D layered double hydroxide composite for the determination of H2O2
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-23
    Jian Yuan, Sheng Xu, Hong-Yan Zeng, Xi Cao, A. Dan Pan, Gao-Fei Xiao, Peng-Xuan Ding

    The composites (LDH-CMC) composed of carboxymethyl chitosan (CMC) and 2D ZnAl layered double hydroxide (LDH) were successfully prepared using the one-step urea method; these composites were characterized by XRD, FT-IR, UV–vis DRS, SEM, BJH/BET, TG-DTG and pHzpc analyses, cyclic voltammetry, and electrochemical impedance spectroscopy. The use of CMC could impact the textural and surface chemical properties of the LDH-CMC composites, where the composites still maintained the 2D layered structure. Incorporating a moderate amount of CMC could increase both the surface area and the permanent charge density of the composites, leading to improved electrochemical performances. The LDH-CMC composite was used as a support matrix for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode to construct a biosensor that provides a biocompatible microenvironment for HRP and a pathway for H2O2 diffusion via the high surface area. The HRP biosensor displayed a satisfactory sensitivity and fast response (<3 s) toward H2O2 over a wide linear range of 0.02–6.0 mmol·L−1 with a low detection limit of 12.4 μmol·L−1, good anti-interference ability and long-term storage stability. The proposed HRP biosensor was found to be a sensitive, rapid, and disposable sensor with low cost, easy preparation and high selectivity; thus, the proposed biosensor can be used for the real-time detection of trace H2O2 in the biological, clinical and environmental fields.

    更新日期:2018-05-05
  • Design and fabrication of an electrochemical aptasensor using Au nanoparticles/carbon nanoparticles/cellulose nanofibers nanocomposite for rapid and sensitive detection of Staphylococcus aureus
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-27
    Saba Ranjbar, Saeed Shahrokhian

    Since that pathogenic bacteria are major threats to human health, this paper describes the fabrication of an effective and durable sensing platform based on gold nanoparticles/carbon nanoparticles/cellulose nanofibers nanocomposite (AuNPs/CNPs/CNFs) at the surface of glassy carbon electrode for sensitive and selective detection of Staphylococcus aureus (S. aureus). The AuNPs/CNPs/CNFs nanocomposite with the high surface area, excellent conductivity, and good biocompatibility was used for self-assembled of the thiolated specific S. aureus aptamer as a sensing element. The surface morphology of AuNPs/CNPs/CNFs nanocomposite was characterized with field emission scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), dynamic light scattering (DLS) and ultraviolet-visible (UV–Vis) spectrophotometric methods. Each aptasensor modification step was monitored with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. The fabricated aptasensor exhibited a wide linear dynamic range (1.2 × 101 to 1.2 × 108) CFU mL−1 with a LOD of 1 CFU mL−1 and was be capable to accurate detection and determination of Staphylococcus aureus in human blood serum as a clinical sample with a complex matrix.

    更新日期:2018-05-03
  • Nanosecond bipolar pulse generators for bioelectrics
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-26
    Shu Xiao, Chunrong Zhou, Enbo Yang, Sambasiva R. Rajulapati

    Biological effects caused by a nanosecond pulse, such as cell membrane permeabilization, peripheral nerve excitation and cell blebbing, can be reduced or cancelled by applying another pulse of reversed polarity. Depending on the degree of cancellation, the pulse interval of these two pulses can be as long as dozens of microseconds. The cancellation effect diminishes as the pulse duration increases. To study the cancellation effect and potentially utilize it in electrotherapy, nanosecond bipolar pulse generators must be made available. An overview of the generators is given in this paper. A pulse forming line (PFL) that is matched at one end and shorted at the other end allows a bipolar pulse to be produced, but no delay can be inserted between the phases. Another generator employs a combination of a resistor, an inductor and a capacitor to form an RLC resonant circuit so that a bipolar pulse with a decaying magnitude can be generated. A third generator is a converter, which converts an existing unipolar pulse to a bipolar pulse. This is done by inserting an inductor in a transmission line. The first phase of the bipolar pulse is provided by the unipolar pulse's rising phase. The second phase is formed during the fall time of the unipolar pulse, when the inductor, which was previously charged during the flat part of the unipolar pulse, discharges its current to the load. The fourth type of generator uses multiple MOSFET switches stacked to turn on a pre-charged, bipolar RC network. This approach is the most flexible in that it can generate multiphasic pulses that have different amplitudes, delays, and durations. However, it may not be suitable for producing short nanosecond pulses (<100 ns), whereas the PFL approach and the RLC approach with gas switches are used for this range. Thus, each generator has its own advantages and applicable range.

    更新日期:2018-05-03
  • 更新日期:2018-05-03
  • A novel drug-free strategy of nano-pulse stimulation sequence (NPSS) in oral cancer therapy: In vitro and in vivo study
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-19
    Jinsong Guo, Feihong Dong, Lian Ding, Kaile Wang, Jue Zhang, Jing Fang

    Nano-pulse stimulation (NPS) is a novel technology to induce cancer apoptosis. In this study, based on the energy-dose effect of NPS, we designed a special NPS sequence (NPSS) with low field intensity. The effectiveness and mechanisms of NPSS on oral cancer therapy were evaluated by cell proliferation assays, microscopic investigation, JC-1 mitochondrial membrane potential assay, tumor inhibition assays, immunohistochemistry (IHC) assay, Ca2+, NOS and ROS detection assays, respectively. The results demonstrated that NPSS treatment significantly inhibited oral cancer growth in vitro and in vivo. Furthermore, we found that NPSS treatment induced an obviously apoptosis and mitochondrial membrane potential (ΔΨm) reduction in Cal-27 cells. Notably, further experiments revealed that the mechanisms of crosstalk signaling between NO, ROS and Ca2+ involvement in NPSS treatment. In conclusion, this is a proof-of-concept study that provides a potential alternative strategy for developing and applying NPSS in oral cancer therapy.

    更新日期:2018-05-01
  • Bioelectrical coupling in multicellular domains regulated by gap junctions: A conceptual approach
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-21
    Javier Cervera, Alexis Pietak, Michael Levin, Salvador Mafe
    更新日期:2018-05-01
  • Biological effects in photodynamic treatment combined with electropermeabilization in wild and drug resistant breast cancer cells
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-19
    Joanna Weżgowiec, Julita Kulbacka, Jolanta Saczko, Joanna Rossowska, Grzegorz Chodaczek, Małgorzata Kotulska

    Electrochemotherapy became one of the therapeutic protocols successfully used in oncology. However, biological effects occurring in cells, especially those which are drug resistant, have not been studied thoroughly. This study presents response of wild and drug resistant breast cancer cells to classical photodynamic therapy with Photofrin or experimental photodynamic therapy with cyanine IR-775, combined with electroporation. Photodynamic reaction or electroporation alone had no cytotoxic effect, but their combination significantly disturbed cellular functions. Applying electroporation allowed the drugs to increase its accumulation, especially for a poorly permeant cyanine in drug resistant cells. FACS analysis showed that even at relatively mild electric field, ca. 90% of cells were permeabilized. High intracellular concentration of drugs triggered the cellular defense system through increased expression of glutathione S-transferase and multidrug resistance proteins (MDR1 and MRP7), particularly in drug resistant cells. Finally, expressively decreased cell metabolism and proliferation, as well as formation of apoptotic bodies and fragmentation of cells were observed after the combined treatment. The results show that electroporation can be used for effective delivery of photosensitizers, even to drug resistant breast cancer cells, which was not tested before. This shows that electro-photodynamic treatment could be a promising approach to overcome a problem of drug resistance in cancer cells.

    更新日期:2018-05-01
  • Investigation of ceramic MFC stacks for urine energy extraction
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-03-21
    Asimina Tremouli, John Greenman, Ioannis Ieropoulos

    Two ceramic stacks, terracotta (t-stack) and mullite (m-stack), were developed to produce energy when fed with neat undiluted urine. Each stack consisted of twelve identical microbial fuel cells (MFCs) which were arranged in cascades and tested under different electrical configurations. Despite voltage reversal, the m-stack produced a maximum power of 800 μW whereas the t-stack produced a maximum of 520 μW after 62.6 h of operation. Moreover, during the operation, both systems were subject to blockage possibly due to struvite. To the Authors' best knowledge, this is the first time that such a phenomenon in ceramic MFC membranes is shown to be the direct result of sub-optimal performance, which confirms the hypothesis that ceramic membranes can continue operating long-term, if the MFCs produce maximum power (high rate of e− transfer). Furthermore, it is shown that once the ceramic membrane is blocked, it may prove difficult to recover in-situ.

    更新日期:2018-05-01
  • Severe microbiologically influenced corrosion of S32654 super austenitic stainless steel by acid producing bacterium Acidithiobacillus caldus SM-1
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-23
    Yuqiao Dong, Baota Jiang, Dake Xu, Chengying Jiang, Qi Li, Tingyue Gu

    Microbiologically influenced corrosion (MIC) of S32654 (654SMO) super austenitic stainless steel (SASS) by acid producing bacterium (APB), Acidithiobacillus caldus SM-1, a strain of sulfur-oxidizing bacteria (SOB) used in biohydrometallurgy field, was investigated using electrochemical measurements and surface characterizations during a 14-day immersion test. The results indicated that S32654 SASS was susceptible to MIC by APB, and A. caldus SM-1 was capable of producing an aggressive acidic environment underneath the biofilm, resulting in the dissolution of the passive film and severe pitting attacks against S32654 SASS, which is commonly regarded as a corrosion resistant material.

    更新日期:2018-05-01
  • On the interaction of the highly charged peptides casocidins with biomimetic membranes
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-19
    Lucia Becucci, Giovanni Aloisi, Andrea Scaloni, Simonetta Caira, Rolando Guidelli

    Casocidin I and II (CI and CII) are structurally related antimicrobial peptides made of 39 and 31 amino acids, respectively, which derive from natural proteolytic processing of αs2-casein and adopt an ordered α-helical structure in biomimetic membranes. Their putative membrane-permeabilizing activity was investigated at Hg-supported self-assembled monolayers (SAMs) and at tethered bilayer lipid membranes (tBLMs); the latter consisted of a monolayer of 2,3,di-O-phytanyl-sn-glycerol-1-tetraethylene-glycol-d,l-α lipoic acid ester thiolipid (DPTL), with a dioleoylphosphatidylcholine (DOPC) or dioleoylphosphatidylserine (DOPS) monolayer on top of it. Interaction of CI and CII with these biomimetic membranes was studied by four electrochemical techniques at pH 3, 5.4 and 6.8. Peptide incorporation in tBLMs was attempted via scans of electrochemical impedance spectra. Experiments demonstrated that CI and CII penetrate SAMs as well as the distal DOPC monolayer of DPTL/DOPC tBLMs, but not the proximal phytanyl monolayer, with the only exception of CII at pH 5.4. Conversely, CII permeabilized DPTL/DOPS tBLMs to a moderate extent at all investigated pH values by forming holes across the membrane, but not ion channels. Structural distribution of charged residues seemed to prevent CII from having a hydrophobic side of the α-helix capable of stabilizing a regular ion channel in the lipid matrix.

    更新日期:2018-04-29
  • Growth and current production of mixed culture anodic biofilms remain unaffected by sub-microscale surface roughness
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-12
    Melanie Pierra, Mehdi Golozar, Xu Zhang, Antonin Prévoteau, Michael de Volder, Dominiek Reynaerts, Korneel Rabaey

    Bioelectrochemical systems couple electricity demand/supply to the metabolic redox reactions of microorganisms. Generally, electrodes act not only as electron acceptors/donors, but also as physical support for an electroactive biofilm. The microorganism-electrode interface can be modified by changing the chemical and/or topographical features of the electrode surface. Thus far, studies have reported conflicting results on the impact of the electrode surface roughness on the growth and current production of biofilms. Here, the surface roughness of the glassy carbon electrodes was successfully modified at the sub-microscale using micro electrodischarge machining, while preserving the surface chemistry of the parent glassy carbon. All microbial electrodes showed similar startup time, maximum current density, charge transport ability across the biofilm and biomass production. Interestingly, an increase in the average surface cavity depth was observed for the biofilm top layer as a function of the electrode surface roughness (from 7 μm to 16 μm for a surface roughness of 5 nm to 682 nm, respectively). These results indicated that the surface roughness at a sub-microscale does not significantly impact the attachment or current production of mixed culture anodic biofilms on glassy carbon. Likely earlier observations were associated with changes in surface chemistry, rather than surface topography.

    更新日期:2018-04-17
  • Different permeabilization patterns of splenocytes and thymocytes to combination of pulsed electric and magnetic field treatments
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-10
    Vitalij Novickij, Auksė Zinkevičienė, Justinas Valiulis, Jurgita Švedienė, Algimantas Paškevičius, Eglė Lastauskienė, Svetlana Markovskaja, Jurij Novickij, Irutė Girkontaitė
    更新日期:2018-04-11
  • Engineered fungus derived FAD-dependent glucose dehydrogenase with acquired ability to utilize hexaammineruthenium(III) as an electron acceptor
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-10
    Madoka Okurita, Nanami Suzuki, Noya Loew, Hiromi Yoshida, Wakako Tsugawa, Kazushige Mori, Katsuhiro Kojima, David C. Klonoff, Koji Sode

    Fungal FAD-dependent glucose dehydrogenases (FADGDHs) are considered to be superior enzymes for glucose sensor strips because of their insensitivity to oxygen and maltose. One highly desirable mediator for enzyme sensor strips is hexaammineruthenium(III) chloride because of its low redox potential and high storage stability. However, in contrast to glucose oxidase (GOx), fungal FADGDH cannot utilize hexaammineruthenium(III) as electron acceptor. Based on strategic structure comparison between FADGDH and GOx, we constructed a mutant of Aspergillus flavus-derived FADGDH, capable of utilizing hexaammineruthenium(III) as electron acceptor: AfGDH-H403D. In AfGDH-H403D, a negative charge introduced at the pathway-entrance leading to the FAD attracts the positively charged hexaammineruthenium(III) and guides it into the pathway. The corresponding amino acid in wild-type GOx is negatively charged, which explains the ability of GOx to utilize hexaammineruthenium(III) as electron acceptor. Electrochemical measurements showed a response current of 46.0 μA for 10 mM glucose with AfGDH-H403D and hexaammineruthenium(III), similar to that with wild-type AfGDH and ferricyanide (47.8 μA). Therefore, AfGDH-H403D is suitable for constructing enzyme electrode strips with hexaammineruthenium(III) chloride as sole mediator. Utilization of this new, improved fungal FADGDH should lead to the development of sensor strips for blood glucose monitoring with increased accuracy and less stringent packing requirements.

    更新日期:2018-04-11
  • Development of an adaptive electroporation system for intratumoral plasmid DNA delivery
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-09
    Douglas W. Brown, Arya J. Bahrami, David A. Canton, Anandaroop Mukhopadhyay, Jean S. Campbell, Robert H. Pierce, Richard J. Connolly

    Intratumoral electroporation of plasmid DNA encoding the proinflammatory cytokine interleukin 12 promotes innate and adaptive immune responses correlating with anti-tumor effects. Clinical electroporation conditions are fixed parameters optimized in preclinical tumors, which consist of cells implanted into skin. These conditions have little translatability to clinically relevant tumors, as implanted models cannot capture the heterogeneity encountered in genetically engineered mouse models or clinical tumors. Variables affecting treatment outcome include tumor size, degree of vascularization, fibrosis, and necrosis, which can result in suboptimal gene transfer and variable therapeutic outcomes. To address this, a feedback controlled electroporation generator was developed, which is capable of assessing the electrochemical properties of tissue in real time. Determination of these properties is accomplished by impedance spectroscopy and equivalent circuit model parameter estimation. Model parameters that estimate electrical properties of cell membranes are used to adjust electroporation parameters for each applied pulse. Studies performed in syngeneic colon carcinoma tumors (MC38) and spontaneous mammary tumors (MMTV-PyVT) demonstrated feedback-based electroporation is capable of achieving maximum expression of reporter genes with significantly less variability and applied energy. These findings represent an advancement to the practice of gene electro-transfer, as reducing variability and retaining transfected cell viability is paramount to treatment success.

    更新日期:2018-04-10
  • A highly sensitive electrochemical biosensor for phenol derivatives using a graphene oxide-modified tyrosinase electrode
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-09
    Yue Wang, Fengge Zhai, Yasushi Hasebe, Hongmin Jia, Zhiqiang Zhang

    The fabrication, characterization and analytical performance were investigated for a phenol biosensor based on the covalent bonding of tyrosinase (TYR) onto a graphene oxide (GO)-modified glassy carbon electrode (GCE) via glutaraldehyde (GA). The surface morphology of the modified electrode was studied by atomic force microscope (AFM) and field-emission scanning electron microscopy (FE-SEM). The fabricated TYR/GA/GO/GCE biosensor showed very good stability, reproducibility, sensitivity and practical usage. The catechol biosensor exhibited a wide sensing linear range from 5 × 10−8 M to 5 × 10−5 M, a lower detection limit of 3 × 10−8 M, a current maximum (Imax) of 65.8 μA and an apparent Michaelis constant (Kmapp) of 169.9 μM.

    更新日期:2018-04-10
  • Gold nanoparticle-decorated reduced-graphene oxide targeting anti hepatitis B virus core antigen
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-09
    Mohamad Farid Abd Muain, Kooi Hoong Cheo, Muhamad Nadzmi Omar, Amir Syahir Amir Hamzah, Hong Ngee Lim, Abu Bakar Salleh, Wen Siang Tan, Asilah Ahmad Tajudin

    Hepatitis B virus core antigen (HBcAg) is the major structural protein of hepatitis B virus (HBV). The presence of anti-HBcAg antibody in a blood serum indicates that a person has been exposed to HBV. This study demonstrated that the immobilization of HBcAg onto the gold nanoparticles-decorated reduced graphene oxide (rGO-en-AuNPs) nanocomposite could be used as an antigen-functionalized surface to sense the presence of anti-HBcAg. The modified rGO-en-AuNPs/HBcAg was then allowed to undergo impedimetric detection of anti-HBcAg with anti-estradiol antibody and bovine serum albumin as the interferences. Upon successful detection of anti-HBcAg in spiked buffer samples, impedimetric detection of the antibody was then further carried out in spiked human serum samples. The electrochemical response showed a linear relationship between electron transfer resistance and the concentration of anti-HBcAg ranging from 3.91 ng mL−1 to 125.00 ng mL−1 with lowest limit of detection (LOD) of 3.80 ng mL−1 at 3 σ m−1. This established method exhibits potential as a fast and convenient way to detect anti-HBcAg.

    更新日期:2018-04-10
  • An impedimetric biosensor for DNA damage detection and study of the protective effect of deferoxamine against DNA damage
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-03-28
    Seyedeh Zeinab Mousavisani, Jahan Bakhsh Raoof, Reza Ojani, Zahra Bagheryan
    更新日期:2018-04-07
  • Controlled synthesis of mixed molecular nanostructures from folate and deferrioxamine-Ga(III) on gold and tuning their performance for cancer cells
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-03-28
    Reza Karimi Shervedani, Fatemeh Yaghoobi, Mostafa Torabi, Fatemeh Rahnemaye Rahsepar, Marzieh Samiei Foroushani
    更新日期:2018-04-07
  • The second phase of bipolar, nanosecond-range electric pulses determines the electroporation efficiency
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-03-29
    Andrei G. Pakhomov, Sergey Grigoryev, Iurii Semenov, Maura Casciola, Chunqi Jiang, Shu Xiao

    Bipolar cancellation refers to a phenomenon when applying a second electric pulse reduces (“cancels”) cell membrane damage by a preceding electric pulse of the opposite polarity. Bipolar cancellation is a reason why bipolar nanosecond electric pulses (nsEP) cause weaker electroporation than just a single unipolar phase of the same pulse. This study was undertaken to explore the dependence of bipolar cancellation on nsEP parameters, with emphasis on the amplitude ratio of two opposite polarity phases of a bipolar pulse. Individual cells (CHO, U937, or adult mouse ventricular cardiomyocytes (VCM)) were exposed to either uni- or bipolar trapezoidal nsEP, or to nanosecond electric field oscillations (NEFO). The membrane injury was evaluated by time-lapse confocal imaging of the uptake of propidium (Pr) or YO-PRO-1 (YP) dyes and by phosphatidylserine (PS) externalization. Within studied limits, bipolar cancellation showed little or no dependence on the electric field intensity, pulse repetition rate, chosen endpoint, or cell type. However, cancellation could increase for larger pulse numbers and/or for longer pulses. The sole most critical parameter which determines bipolar cancellation was the phase ratio: maximum cancellation was observed with the 2nd phase of about 50% of the first one, whereas a larger 2nd phase could add a damaging effect of its own. “Swapping” the two phases, i.e., delivering the smaller phase before the larger one, reduced or eliminated cancellation. These findings are discussed in the context of hypothetical mechanisms of bipolar cancellation and electroporation by nsEP.

    更新日期:2018-04-06
  • Viability and membrane lipid composition under a 57 mT static magnetic field in Salmonella Hadar
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-03-29
    Ramla Ben Mouhoub, Alya El May, Imen Boujezza, Mohamed Marouen Sethom, Moncef Feki, Ahmed Landoulsi

    The aim of this work is to demonstrate the effects of a static magnetic field (SMF) with an induction 12 equal to 57 mT on the viability and membrane lipid composition of Salmonella Hadar. Results showed an increase in the viability of exposed bacteria compared to controls after 9 h of exposure. Analysis with gas chromatography of total lipids (TLs) and different fractions of phospholipids: phosphatidylglycerols (PGs), phosphatidylethanolamines (PEs), and cardiolipins (CLs), separated by thin layer chromatography revealed changes in fatty acid levels during exposure. For TLs, the unsaturated fatty acids/saturated fatty acids ratio (UFAs/SFAs) had significantly increased after 9 h of exposure. The variation of this ratio seems to be essentially due to the increase of the proportion of unsaturated fatty acids with 18 carbons, in particular C18:1. The analyses of fatty acid composition carried out on the scale of each fraction of phospholipids showed that CLs contributed significantly to the increase of the proportion of the unsaturated fatty acids between 6 and 9 h of exposure thanks to their unsaturated chains with 18 carbons (especially C18:2). CLs appear to be the main phospholipid involved in the adaptation of S. Hadar membranes to the SMF.

    更新日期:2018-04-06
  • An insoluble iron complex coated cathode enhances direct electron uptake by Rhodopseudomonas palustris TIE-1
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-05
    Karthikeyan Rengasamy, Tahina Ranaivoarisoa, Rajesh Singh, Arpita Bose

    Microbial electrosynthesis (MES) is a promising bioelectrochemical approach to produce biochemicals. A previous study showed that Rhodopseudomonas palustris TIE-1 can directly use poised electrodes as electron donors for photoautotrophic growth at cathodic potentials that avoid electrolytic H2 production (photoelectroautotrophy). To make TIE-1 an effective biocatalyst for MES, we need to improve its electron uptake ability and growth under photoelectroautotrophic conditions. Because TIE-1 interacts with various forms of iron while using it as a source of electrons for photoautotrophy (photoferroautotrophy), we tested the ability of iron-based redox mediators to enhance direct electron uptake. Our data show that soluble iron cannot act as a redox mediator for electron uptake by TIE-1 from a cathode poised at +100 mV vs. Standard Hydrogen electrode. We then tested whether an immobilized iron-based redox mediator Prussian blue (PB) can enhance electron uptake by TIE-1. Chronoamperometry indicates that cathodic current uptake by TIE-1 increased from 1.47 ± 0.04 to 5.6 ± 0.09 μA/cm2 (3.8 times). Overall, our data show that immobilized PB can enhance direct electron uptake by TIE-1.

    更新日期:2018-04-06
  • Proteus vulgaris - Pt electrode system for urea to nitrogen conversion in synthetic urine
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-04
    Myreisa Morales-Cruz, Marjorie López-Nieves, Roberto Morales-Hernández, Gian C. Rivera-Crespo, Gary A. Toranzos, Ileana González-González, Carlos R. Cabrera

    One of the most challenging problems when trying to recycle urine for different purposes is the removal of urea. In this project we studied an ureolysis system using the bacterium Proteus vulgaris for the transformation of urea to ammonia and its subsequent oxidation to nitrogen at a Pt working electrode. Our system was tested under different pH, microbial reaction times, and urea and bacteria concentrations. Our results indicate that a pH 8 is optimal for the combined Proteus vulgaris urease activity and the ammonia oxidation reaction at a Pt electrode. The reaction time and concentration dependence on the ammonia oxidation reaction current densities was also studied. Results showed limited ammonia oxidation under high urea concentrations in ~2.5 × 109 cfu/mL Proteus vulgaris in synthetic urine.

    更新日期:2018-04-06
  • Elucidation of the intra- and inter-molecular electron transfer pathways of glucoside 3-dehydrogenase
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-03-07
    Ryota Miyazaki, Tomohiko Yamazaki, Keiichi Yoshimatsu, Katsuhiro Kojima, Ryutaro Asano, Koji Sode, Wakako Tsugawa

    Glucoside 3‑dehydrogenase (G3DH) is a flavin adenine dinucleotide (FAD)-containing oxidoreductase that catalyzes the oxidation of the hydroxy group on the C-3 position of pyranose and shows broad substrate specificity by oxidizing many saccharides. Due to unique site specificity and wide substrate specificity, G3DHs can be used for synthesis of sugar derivatives, anodic catalysis in biofuel cells, multi-sugar analysis using enzyme electrode, and for enzymatic detection of 1,5‑anhydro‑d‑glucitol, a clinical marker for diabetes. However, few studies have focused on the fundamental biochemical properties of G3DH, including its electron transfer pathway. In this study, we isolated the G3DH gene from Rhizobium radiobacter, a homologue of marine bacterial G3DH, and reported that the isolated gene fragment contains the genes encoding the G3DH catalytic subunit (subunit I), G3DH hitch-hiker subunit (subunit II), and cytochrome c-like molecule (CYTc). Furthermore, we report the recombinant expression of G3DH from R. radiobacter in Escherichia coli, the characterization of recombinant G3DH and the investigation of the molecular electron pathway of G3DH. We first prepared the G3DH subunit I-II complex using a co-expression vector for both subunits. The G3DH subunit I-II complex showed dye-mediated G3DH activity toward methyl‑α‑d‑glucoside (MαG). Electron paramagnetic resonance (EPR) and inductively coupled plasma optical emission spectroscopy (ICP-OES) analyses revealed that subunit I contains an iron-sulfur cluster. We, then, prepared recombinant CYTc and revealed that it is capable of accepting electrons from the catalytic subunit of G3DH by absorption spectrum analysis. These results suggested that R. radiobacter G3DH possesses an iron‑sulfur cluster that may play an important role in the electron transfer from FAD to cytochrome c like molecule, which is an external electron acceptor of G3DH. Furthermore, we demonstrated that CYTc mediate the electron transfer from G3DH to electrode without the artificial electron mediator.

    更新日期:2018-04-04
  • Construction of photo-driven bioanodes using thylakoid membranes and multi-walled carbon nanotubes
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-04-03
    Ryosuke Takeuchi, Arato Suzuki, Kento Sakai, Yuki Kitazumi, Osamu Shirai, Kenji Kano

    A photo-driven bioanode was constructed using the thylakoid membrane from spinach, carbon nanotubes, and an artificial mediator. By considering a linear free-energy relationship in the electron transfer from the thylakoid membrane to the mediators, and the oxygen resistance of the reduced mediators, 1,2-naphthoquinone was selected as the most suitable mediator for the photo-driven bioanode. Water-dispersed multi-walled carbon nanotubes served as scaffolds to hold the thylakoid membrane on a porous electrode. The constructed photo-driven bioanode exhibited a photocurrent density of over 100 μA cm−2 at a photon flux density of 1500 μmol m−2 s−1.

    更新日期:2018-04-04
  • Plasma membrane depolarization and permeabilization due to electric pulses in cell lines of different excitability
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-03-21
    Janja Dermol-Černe, Damijan Miklavčič, Matej Reberšek, Primož Mekuč, Sylvia M. Bardet, Ryan Burke, Delia Arnaud-Cormos, Philippe Leveque, Rodney O'Connor
    更新日期:2018-04-03
  • Electrochemical immunosensor for highly sensitive and quantitative detection of tumor necrosis factor-α in human serum
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-03-20
    Ajay Kumar Yagati, Min-Ho Lee, Junhong Min

    Highly sensitive and selective biosensors for accurate determination of specific protein biomarkers at low levels in serum are a prerequisite for the present healthcare systems. Therefore, here we developed a label-free impedimetric method for tumor necrosis factor-alpha (TNF-α) detection using reduced graphene oxide (RGO) with gold nanoparticles (AuNP) on an indium tin oxide (ITO) microdisk electrodes. The detection mechanism relies on resistance change occurs due to [Fe(CN)6]3−/4− redox probe movement towards the conductive channels of the AuNP-RGO films gated by the recognition of the target biomarker by its anti-TNF-α antibody. The conductivity of the AuNP-RGO structures enhanced by 10-fold in comparison with bare electrode chips. The observed resistance changes at 2 Hz (ΔR at 2Hz) enabled the quantification of various concentrations of TNF-α in human serum (CHS-TNF-α). The antibody-adsorbed electrode showed a good increment of resistance change (ΔR) with additions of antigen concentration. The sensor possesses a linear range of 1–1000 pg/ml had a detection limit of 0.67 pg/ml (38.51 fM) and 0.78 pg/ml (44.83 fM) in PBS and human serum, respectively.

    更新日期:2018-03-28
  • Expressional changes in stemness markers post electrochemotherapy in pancreatic cancer cells
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-03-15
    M. Shahzad Ali, Kheshwant S. Gill, Giuseppe Saglio, Daniela Cilloni, Declan M. Soden, Patrick F. Forde

    Pancreatic cancer is one of the most lethal cancers with high metastatic potential and strong chemoresistance. The capability of a tumor to grow and propagate is dependent on a small subset of cells within a tumor, termed cancer stem cells. Cancer stem cells exhibit great tumorigenicity and are closely correlated with drug resistance and tumor recurrence. The aim of our study was to illustrate electrochemotherapy as an effective treatment for pancreatic cancer along with the expression change in stemness genes (Nanog, Sox2 and Oct3/4) in pancreatic cancer cells post electrochemotherapy with bleomycin, cisplatin and oxaliplatin. Our results showed the enhanced expression of Nanog and decreased expression level of Oct3/4 after electrochemotherpy. We thus propose that these stemness markerS may have important roles in the initiation and/or recurrence of pancreatic cancer, and consequently may serve as important molecular diagnostics and/or therapeutic targets for the development of novel treatment strategies in pancreatic cancer patients. In conclusion, targeting these stemness factors could potentially improve electrochemotherapy as a treatment and preventing recurrence.

    更新日期:2018-03-24
  • Gold nanoparticles/4-aminothiophenol interfaces for direct electron transfer of horseradish peroxidase: Enzymatic orientation and modulation of sensitivity towards hydrogen peroxide detection
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-03-17
    G.A. Huerta-Miranda, A.A. Arrocha-Arcos, M. Miranda-Hernández

    Hydrogen peroxide electrochemical detection by horseradish peroxidase has been widely studied. The use of gold nanoparticles to prepare electrode/enzyme bioconjugates has attracted attention due to their catalytic properties. In this work, it is reported the use of gold nanoparticles and 4-aminothiophenol as a scaffold to obtain a suitable matrix for enzyme bioconjugation with horseradish peroxidase. A critical factor in biosensors design and development is the enzymatic electrochemical activity understanding. Comparison of voltammetric studies of the heme prosthetic group showed a reversible electrochemical behavior when the enzymes were immobilized in a well-dispersed gold deposit; on the other hand, a discrete redox response was observed on a randomly deposited gold electrode. These results show that the distance between enzymes is essential. Hydrogen peroxide catalysis and the enzymatic behavior were analyzed considering two types of nanoparticles dispositions. The catalytic behavior observed in the well-dispersed nanoparticles configuration suggests a preserved enzyme folding, a decrease of steric impediments, and appears to be a better immobilization strategy. In contrast, the randomly electrodeposited gold electrode decreased the enzyme orientation and the electrochemical activity. The advantages of this methodology are the electrode fabrication affordable cost and the enzymatic direct electron transfer response improvement.

    更新日期:2018-03-23
  • Recommendations and requirements for reporting on applications of electric pulse delivery for electroporation of biological samples
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-03-12
    M. Cemazar, G. Sersa, W. Frey, D. Miklavcic, J. Teissié

    Electric field-induced membrane changes are an important approach in the life sciences. However, the developments in knowledge and translational applications face problems of reproducibility. Indeed, a quick survey of the literature reveals a lack of transparent and comprehensive reporting of essential technical information in many papers. Too many of the published scientific papers do not contain sufficient information for proper assessment of the presented results. The general rule/guidance in reporting experimental data should require details on exposure conditions such that other researchers are able to evaluate, judge and reproduce the experiments and data obtained. To enhance dissemination of information and reproducibility of protocols, it is important to agree upon nomenclature and reach a consensus on documentation of experimental methods and procedures. This paper offers recommendations and requirements for reporting on applications of electric pulse delivery for electroporation of biological samples in life science.

    更新日期:2018-03-22
  • 更新日期:2018-03-20
  • A novel model for predicting bioelectrochemical performance of microsized-MFCs by incorporating bacterial chemotaxis parameters and simulation of biofilm formation
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-03-12
    Mohammad Kalantar, Mohammad Mahdi Mardanpour, Soheila Yaghmaei

    Bacterial transport parameters play a fundamental role in microbial population dynamics, biofilm formation and bacteria dispersion. In this study, the novel model was extended based on the capability of microsized microbial fuel cells (MFCs) as amperometric biosensors to predict the cells' chemotactic and bioelectrochemical properties. The model prediction results coincide with the experimental data of Shewanella oneidensis and chemotaxis mutant of P. aeruginosa bdlA and pilT strains, indicating the complementary role of numerical predictions for bioscreening applications of microsized MFCs. Considering the general mechanisms for electron transfer, substrate biodegradation, microbial growth and bacterial dispersion are the main features of the presented model. In addition, the genetic algorithm method was implemented by minimizing the objective function to estimate chemotaxis properties of the different strains. Microsized MFC performance was assessed by analyzing the microbial activity in the biofilm and the anolyte.

    更新日期:2018-03-19
  • 更新日期:2018-03-15
  • Bacterial electroactivity and viability depends on the carbon nanotube-coated sponge anode used in a microbial fuel cell
    Bioelectrochemistry (IF 3.346) Pub Date : 
    Hanyue Ma, Tian Xia, Congcong Bian, Huihui Sun, Zhuang Liu, Chao Wu, Xia Wang, Ping Xu

    The anode material is vital to improve the power generation of a microbial fuel cell (MFC). In this study, a carbon nanotube (CNT)-coated sponge with macro-porous structure, high surface area, and high conductivity was constructed as an anode to encapsulate Escherichia coli K12 (E. coli K12) cells. To achieve high power generation of the MFC, the optimal concentration of the CNT coating the sponge was found to be 30 mg mL−1. At this concentration, a maximum power density of 787 W m−3 and a chemical oxygen demand (COD) removal of 80.9% were obtained with a long stable electricity generation process in batch mode. This indicates that the biofilm on the CNT (30 mg mL−1)-coated sponge possessed excellent electroactivity and stability. Scanning electron microscope (SEM) images confirmed that the CNT-coated sponge provided a suitable microenvironment for E. coli K12 cells to maintain their attachment and colonization. Additionally, a CNT-dependent viability phenomenon of the E. coli K12 cells was discovered after electricity generation. This CNT-dependent viability of the E. coli K12 cells was stable and sustainable after storage at −20 °C in a milk tube for one year.

    更新日期:2018-03-07
  • Ethanol generation, oxidation and energy production in a cooperative bioelectrochemical system
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-02-27
    Kamila C. Pagnoncelli, Andressa R. Pereira, Graziela C. Sedenho, Thiago Bertaglia, Frank N. Crespilho

    Integrating in situ biofuel production and energy conversion into a single system ensures the production of more robust networks as well as more renewable technologies. For this purpose, identifying and developing new biocatalysts is crucial. Herein, is reported a bioelectrochemical system consisting of alcohol dehydrogenase (ADH) and Saccharomyces cerevisiae, wherein both function cooperatively for ethanol production and its bioelectrochemical oxidation. Here, it is shown that it is possible to produce ethanol and use it as a biofuel in a tandem manner. The strategy is to employ flexible carbon fibres (FCF) electrode that could adsorb both the enzyme and the yeast cells. Glucose is used as a substrate for the yeast for the production of ethanol, while the enzyme is used to catalyse the oxidation of ethanol to acetaldehyde. Regarding the generation of reliable electricity based on electrochemical systems, the biosystem proposed in this study operates at a low temperature and ethanol production is proportional to the generated current. With further optimisation of electrode design, we envision the use of the cooperative biofuel cell for energy conversion and management of organic compounds.

    更新日期:2018-03-06
  • Cytocompatible tantalum films on Ti6Al4V substrate by filtered cathodic vacuum arc deposition
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-02-27
    Ay Ching Hee, Huiliang Cao, Yue Zhao, Sina S. Jamali, Avi Bendavid, Philip J. Martin

    Tantalum films were deposited on negatively biased Ti6Al4V substrates using filtered cathodic vacuum arc deposition to enhance the corrosion resistance of the Ti6Al4V alloy. The effect of substrate voltage bias on the microstructure, mechanical and corrosion properties was examined and the cytocompatibility of the deposited films was verified with mammalian cell culturing. The Ta films deposited with substrate bias of −100 V and −200 V show a mixture of predominantly β phase and minority of α phase. The Ta/−100 V film shows adhesive failure at the Ti/Ta interface and a cohesive fracture is observed in Ta/−200 V film. The Ta/−100 V showed a significant improvement in corrosion resistance, which is attributed to the stable oxide layer. The in-vitro cytocompatibility of the materials was investigated using rat bone mesenchymal stem cells, and the results show that the Ta films have no adverse effect on mammalian cell adhesion and spreading proliferation.

    更新日期:2018-02-28
  • Light-dependent processes on the cathode enhance the electrical outputs of sediment microbial fuel cells
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-02-27
    Ivo Bardarov, Mario Mitov, Desislava Ivanova, Yolina Hubenova
    更新日期:2018-02-28
  • 更新日期:2018-02-16
  • Effect of surface nano/micro-structuring on the early formation of microbial anodes with Geobacter sulfurreducens: Experimental and theoretical approaches
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-02-15
    Pierre Champigneux, Cyril Renault-Sentenac, David Bourrier, Carole Rossi, Marie-Line Delia, Alain Bergel

    Smooth and nano-rough flat gold electrodes were manufactured with controlled Ra of 0.8 and 4.5 nm, respectively. Further nano-rough surfaces (Ra 4.5 nm) were patterned with arrays of micro-pillars 500 μm high. All these electrodes were implemented in pure cultures of Geobacter sulfurreducens, under a constant potential of 0.1 V/SCE and with a single addition of acetate 10 mM to check the early formation of microbial anodes. The flat smooth electrodes produced an average current density of 0.9 A·m−2. The flat nano-rough electrodes reached 2.5 A·m−2 on average, but with a large experimental deviation of ±2.0 A·m−2. This large deviation was due to the erratic colonization of the surface but, when settled on the surface, the cells displayed current density that was directly correlated to the biofilm coverage ratio. The micro-pillars considerably improved the experimental reproducibility by offering the cells a quieter environment, facilitating biofilm development. Current densities of up to 8.5 A·m−2 (per projected surface area) were thus reached, in spite of rate limitation due to the mass transport of the buffering species, as demonstrated by numerical modelling. Nano-roughness combined with micro-structuring increased current density by a factor close to 10 with respect to the smooth flat surface.

    更新日期:2018-02-16
  • Enhanced electrosynthesis performance of Moorella thermoautotrophica by improving cell permeability
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-02-08
    Shanshan Chen, Yanlun Fang, Xianyue Jing, Hailin Luo, Jing Chen, Shungui Zhou

    Microbial electrosynthesis systems (MES) are promising devices in which microbes obtain electrons from electrodes to produce extracellular multicarbon compounds. This study investigates whether improvement in cell permeability can enhance electrosynthesis performance of Gram-positive Moorella thermoautotrophica in MES. Results showed that when ≤30 mg/L penicillin was added, the cell permeability was doubled, and the electron uptake per biomass (including both cathode-associated biomass and suspended biomass) was 1.84 times that of the control, while formate and acetate production rates per biomass were 1.96 and 2.23 times those of the control, respectively. Enhanced cell permeability caused higher redox activities of outmost cytochrome C and increased release of redox electron shuttles, both of which were beneficial to extracellular electron uptake. Coulombic efficiencies increased from 73% ± 3% to 88% ± 3% with better cell permeability, demonstrating that higher proportion of electrical energy recovered in the chemical-production reaction. This research demonstrates that making a moderate decrease in peptidoglycan of cell walls to improve cell permeability can enhance electron uptakes and chemical production rates of Gram-positive microbes in MES, which would serve as a base for the future genetic modification study of superior electrosynthesis strains.

    更新日期:2018-02-15
  • Development of a glucose sensor employing quick and easy modification method with mediator for altering electron acceptor preference
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-02-09
    Mika Hatada, Noya Loew, Yuka Takahashi, Junko Okuda-Shimazaki, Wakako Tsugawa, Ashok Mulchandani, Koji Sode

    Enzyme based electrochemical biosensors are divided into three generations according to their type of electron transfer from the cofactors of the enzymes to the electrodes. Although the 3rd generation sensors using direct electron transfer (DET) type enzymes are ideal, the number of enzyme types which possess DET ability is limited. In this study, we report of a glucose sensor fabricated using mediator-modified glucose dehydrogenase (GDH), that was fabricated by a new quick-and-easy method using the pre-functionalized amine reactive phenazine ethosulfate (arPES). Thus mediator-modified glucose dehydrogenase (GDH) obtained the ability to transfer electrons to bulky electron acceptors as well as electrodes. The concentration of glucose was successfully measured using electrodes with immobilized PES-modified GDH, without addition of external electron mediators. Therefore, continuous monitoring systems can be developed based on this “2.5th generation” electron transfer principle utilizing quasi-DET. Furthermore, we successfully modified two other diagnostically relevant enzymes, glucose 3-dehydrogenase and lactate oxidase, with PES. Therefore, various kinds of diagnostic enzymes can achieve quasi-DET ability simply by modification with arPES, suggesting that continuous monitoring systems based on the 2.5th generation principle can be developed for various target molecules.

    更新日期:2018-02-10
  • Microbial desalination cell with sulfonated sodium polyether ether ketone as cation exchange membranes for enhancing power generation and desalination
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-02-09
    Francisco Lopez Moruno, Juan E. Rubio, Plamen Atanassov, José M. Cerrato, Christopher G. Arges, Carlo Santoro

    Microbial desalination cell (MDC) is a bioelectrochemical system capable of oxidizing organics, generating electricity and reduce the salinity content into the desalination chamber. As it is designed, anion and cation exchange membranes play an important role on the selective removal of ions from the desalination chamber. In this work, sulfonated sodium (Na+) poly(ether ketone) (SPEEK) cation exchange membranes (CEM) were tested in combination with quaternary ammonium chloride poly(2,6-dimethyl 1,4-phenylene oxide) (QAPPO) anion exchange membrane (AEM). Non-patterned and patterned (with increased, lateral different topographical features) CEMs were investigated and assessed in this work. The results were contrasted against a commercially available CEM. This work used real seawater from the Pacific Ocean in the desalination chamber. The results displayed a high desalination rate and power generation for all the membranes, with a maximum of 78.6 ± 2.0% in salinity reduction and 235 ± 7 mW m−2 in power generation for the MDCs with a non-patterned (i.e., flat) CEM. Desalination rate and power generation achieved are higher with synthesized SPEEK membranes when compared with an available commercial CEM. An optimized combination of these types of membranes substantially improves the performances of this bioelectrochemical system, making the system more suitable for real applications.

    更新日期:2018-02-10
  • Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-02-09
    Ana S. Peinetti, Helena Ceretti, Martín Mizrahi, Graciela A. González, Silvana A. Ramírez, Félix G. Requejo, Javier M. Montserrat, Fernando Battaglini

    Polyvalent gold nanoparticle oligonucleotide conjugates are subject of intense research. Even though 2 nm diameter AuNPs have been previously modified with DNA, little is known about their structure and electrochemical behavior. In this work, we examine the influence of different surface modification strategies on the interplay between the meso-organization and the molecular recognition properties of a 27-mer DNA strand. This DNA strand is functionalized with different sulfur-containing moieties and immobilized on 2 nm gold nanoparticles confined on a nanoporous alumina, working the whole system as an electrode array. Surface coverages were determined by EXAFS and the performance as recognition elements for impedance-based sensors is evaluated. Our results prove that low DNA coverages on the confined nanoparticles prompt to a more sensitive response, showing the relevance in avoiding the DNA strand overcrowding. The system was able to determine a concentration as low as 100 pM of the complementary strand, thus introducing the foundations for the construction of label-free genosensors at the nanometer scale.

    更新日期:2018-02-09
  • Preparation and application of a carbon paste electrode modified with multi-walled carbon nanotubes and boron-embedded molecularly imprinted composite membranes
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-01-31
    Hongjuan Wang, Duo Qian, Xilin Xiao, Chunyan Deng, Lifu Liao, Jian Deng, Ying-Wu Lin
    更新日期:2018-02-06
  • A new composite consisting of electrosynthesized conducting polymers, graphene sheets and biosynthesized gold nanoparticles for biosensing acute lymphoblastic leukemia
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-01-16
    Mohammad Mazloum-Ardakani, Behnaz Barazesh, Alireza Khoshroo, Mohammad Moshtaghiun, Mohammad Hasan Sheikhha
    更新日期:2018-02-04
  • Comparative performances of microbial capacitive deionization cell and microbial fuel cell fed with produced water from the Bakken shale
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-01-11
    Namita Shrestha, Govinda Chilkoor, Joseph Wilder, Zhiyong Jason Ren, Venkataramana Gadhamshetty

    This study evaluates and compares the performance of microbial fuel cells (MFCs) and microbial capacitive deionization cells (MCDCs) fed with wastewater produced from the Bakken shale. The produced water was characterized by high levels of dissolved solids and chemical oxygen demand (COD). Two-compartment MFCs and three-compartment MCDCs were evaluated under batch-fed mode using mixed microbial consortia in the anode, ferricyanide in the cathode, and produced water as the electrolyte in the anode and capacitive deionization units. COD removal in the MFCs was 88%, while that in the MCDCs was limited to 76%. The lower performance of the MCDCs was due to the large impedance (6600 Ω cm2) compared with the MFCs (870 Ω cm2). However, the MCDCs achieved two-fold higher removal of dissolved solids. Both the MFCs and MCDCs suffered from a higher impedance induced by fouling in the latter stages of the operation.

    更新日期:2018-02-04
  • Electrochemical removal of biofilms from titanium dental implant surfaces
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-01-31
    Sebastian Schneider, Michael Rudolph, Vanessa Bause, Andreas Terfort
    更新日期:2018-02-04
  • Electrochemical corrosion, wear and cell behavior of ZrO2/TiO2 alloyed layer on Ti-6Al-4V
    Bioelectrochemistry (IF 3.346) Pub Date : 2018-02-03
    Jianfang Li, Xiaojing He, Guannan Zhang, Ruiqiang Hang, Xiaobo Huang, Bin Tang, Xiangyu Zhang

    Ti-6Al-4V (TC4) has received increasing attention as biomaterial but also raised concerns about the long-term safety of releasing of metal ions and poor wear resistance. In this work, an ZrO2/TiO2 alloyed layer was prepared on TC4 by plasma surface alloying with Zr and subsequently annealed in the air for improved corrosion and wear resistant. To assess the corrosion performance of the alloyed layer, the specimens were measured by open circuit potential, electrochemical impedance spectroscopy and potentiodynamic polarization in simulated body fluid solution. The result shows that the ZrO2/TiO2 alloyed layer exhibits strikingly high polarization resistance, wide passive region and very low current density, indicating the excellent corrosion resistance. The layer also displays significant improvement of wear resistance. Furthermore, the alloyed layer restricts cell adhesion and spreading. We infer that the ZrO2/TiO2 alloyed layer might be potentially useful implanted devices such as biosensors, bioelectronics or drug delivery devices.

    更新日期:2018-02-04
Some contents have been Reproduced with permission of the American Chemical Society.
Some contents have been Reproduced by permission of The Royal Society of Chemistry.
化学 • 材料 期刊列表