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Analysis of vitamin D and its metabolites in biological samples – Part I: Optimization and comparison of UHPSFC-MS/MS and UHPLC-MS/MS methods J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-12 Veronika Pilařová, Bárbara Socas-Rodríguez, Lucie Nováková, Sofia Essén, Cecilia Holm, Charlotta Turner, Margareta Sandahl
Fat-soluble vitamin D is an essential bioactive compound important for human health. Insufficient vitamin D levels can result not only in bone disease but also in other disorders, such as cancer, metabolic disorders, and diseases related to poor immune function. The current methods commonly used for vitamin D analysis are often applied to determine the levels of the most abundant metabolite in plasma
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Analysis of vitamin D and its metabolites in biological samples – Part II: Optimization of a sample preparation method for liver tissue J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-11 Veronika Pilařová, Bárbara Socas-Rodríguez, Lucie Nováková, Cecilia Holm, Margareta Sandahl, Charlotta Turner
Extraction of vitamin D, including its hydroxylated and esterified metabolites, from soft tissues such as the liver is challenging due to the lipophilic character of matrix and analytes that are expected in very low concentration levels.
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Exploring the potential of using ion mobility-mass spectrometry to separate matrix interferences from analytes in food control J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-11 Sjors Rasker, Marco H. Blokland, Toine F.H. Bovee, Ane Arrizabalaga-Larrañaga
During residue analysis in complex matrices for food safety purposes, interfering signals can sometimes overlap with those of the analyte of interest. Access to an additional separation dimension besides chromatographic and mass separation, such as ion mobility, can aid in removing interfering signals, allowing for correct analyte identification in these cases. In our laboratory, during routine LC-MS/MS
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Sensitive, reliable and simultaneous determination of Fingolimod and Citalopram drug molecules used in multiple sclerosis treatment based on magnetic solid phase extraction and HPLC-PDA J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-11 Esra Durgun, Halil İbrahim Ulusoy, İbrahim Narin
An analytical methodology has been developed for trace amounts of Fingolimod (FIN) and Citalopram (CIT) drug molecules based on magnetic solid phase extraction (MSPE) and high performance liquid chromatographic determination with photodiode array detector (HPLC-DAD). Fingolimod is used in treatment of Multiple sclerosis (MS) disease and sometimes antidepressant drugs such as citalopram accompany to
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Pro-inflammatory enzyme inhibition of lipoxygenases by flavonoid rich extract from Artemisia vulgaris J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-10 Jin Ru Lim, Lee Suan Chua, Azizul Azri Mustaffa
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Generation of affinity maps for thiazolidinediones with human serum albumin using affinity microcolumns. I. Studies of effects by glycation on multisite drug binding J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-03 Sadia Sharmeen, Ashley Woolfork, David S. Hage
Human serum albumin (HSA) is known to undergo modifications by glucose during diabetes. This process produces glycated HSA that can have altered binding to some drugs. In this study, high-performance affinity microcolumns and competition studies were used to see how glycation affects the binding by two thiazolidinedione-class drugs (i.e., pioglitazone and rosiglitazone) at specific regions of HSA.
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Composition and nutritional values of fatty acids in marine organisms by one-step microwave-assisted extraction/derivatization and comprehensive two-dimensional gas chromatography -flame ionization detector J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-02 Donatella Ferrara, Mirco Cescon, Giulia Giacoppo, Valentina Costa, Giorgia Purcaro, Natasha Damiana Spadafora, Chiara Cordero, Luisa Pasti, Alberto Cavazzini, Marco Beccaria
This work reports the characterization of the lipidic fraction of seven species of marine organisms gathered along the shoreline of the Po Delta Park of Emilia-Romagna Region (Italy) and of the north Adriatic Sea. Two species of oysters ( and ), two species of clams ( and ), one species of mussel (), one species of macroalgae (), and one species of spiny dogfish () were analyzed to characterize their
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New azaspiracid analogues detected as bi-charged ions in Azadinium poporum (Amphidomataceae, Dinophyceae) isolated from Japanese coastal waters J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-02 Mayu Ozawa, Hajime Uchida, Ryuichi Watanabe, Satoshi Numano, Ryoji Matsushima, Hiroshi Oikawa, Kazuya Takahashi, Wai Mun Lum, Garry Benico, Mitsunori Iwataki, Toshiyuki Suzuki
Lipophilic marine biotoxin azaspiracids (AZAs) are produced by dinoflagellates and . Recently, several strains of were isolated from Japanese coastal waters, and detailed toxin profiles of two strains (mdd421 and HM536) among them were clarified by several detection techniques on liquid chromatography-tandem mass spectrometry (LC-MS/MS) and liquid chromatography-quadrupole time of flight mass spectrometry
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Validation of a human saliva model for the determination of leachable monomers and other chemicals from dental materials J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-01 Lene A. Grutle, Heidi V. Holm, Hilde B.M. Kopperud, Silvio Uhlig
This study aimed to prove the validity of a mixture of chemicals, including salts, small organic molecules, mucin, and α-amylase, as saliva surrogate (“artificial saliva”) for assessing leakage of methacrylate monomers and other constituents from dental materials. To achieve this, we developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of
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Study on material basis and anti-hypertensive metabolomics of Zhengan-Xifeng-Tang(ZXT): A comparison between ZXT decoction and granules J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-01 Haichao Li, Lihua Wang, Hao Zhang, Wenchi Yu, Yunlun Li, Haiqing Jiang, Danyang Wang, Yu Wang
High blood pressure is a serious human health problem and one of the leading risk factors for fatal complications in cardiovascular disease. The ZXT granules were prepared based on the Zhengan-Xifeng-Tang (ZXT) decoction. However, the therapeutic effects of ZXT granules on spontaneous hypertension and the metabolic pathways in which they may intervene are unclear. The aim of this study was to investigate
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Effective separation of maslinic acid and oleanolic acid from olive pomace using high-speed shear off-line coupled with high-speed countercurrent chromatography and their antibacterial activity test J. Chromatogr. B (IF 3.0) Pub Date : 2024-03-01 Kun Yang, Sheng-Bing Wang, Dong Pei, Lu-Mei Pu, Xin-Yi Huang
In this work, a high-speed shear extraction off-line coupling high-speed countercurrent chromatography method was developed to separate maslinic acid and oleanolic acid from olive pomace. To improve extraction efficiency, the polar disparity between maslinic acid and oleanolic acid necessitated the concurrent utilization of both polar and non-polar solvents during high-speed shear extraction. Then
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Rapid evaluation of PHD2 inhibitory activity of natural products based on capillary electrophoresis online stacking strategy J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-28 Ying Zhang, Chun-Yan Sang, Xing-Rong Wang, Cheng-Bo Wang, Xian-Hua Meng, Wei-Feng Wang, Jun-Li Yang
Prolyl hydroxylase domain 2 (PHD2) is an important enzyme in the human body that perceives changes in oxygen concentration and regulates response in hypoxic environments. Evaluation of PHD2 inhibitory activity of natural products is crucial for drug development of hypoxia related diseases. At present, the detection of low concentration of -ketoglutaric acid (the substrate of PHD2 enzymatic reaction)
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Development and validation of LC-MS/MS for quantifying omadacycline from stool for gut microbiome studies J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-28 Chenlin Hu, Weiqun Wang, Jinhee Jo, Kevin W. Garey
This study developed and validated a new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify omadacycline and its epimerization in stool to facilitate microbiome studies. Omadacycline was extracted in a methanol-water-ethylenediaminetetraacetic acid (ETDA) solvent containing deuterated omadacycline as internal standard, followed by dilution. In an optimal gradient elution mode
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Accurate redox state indication by in situ derivatization with n-ethylmaleimide - Profiling of transsulfuration and glutathione pathway metabolites by UPLC-MS/MS J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-28 Mathias Langner, Dennis Fröbel, Jana Helm, Triantafyllos Chavakis, Mirko Peitzsch, Nicole Bechmann
Reduced and oxidized glutathione play an important role for the intracellular detoxification of reactive oxygen species. The iron-dependent formation of such reactive oxygen species in conjunction with the inhibition of the redox-balancing enzyme glutathione peroxidase 4 underlie an imbalance in the cellular redox state, thereby resulting in a non-apoptotic form of cell death, defined as ferroptosis
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A simple tandem bioassay-guided SCX-RP SPE fractionation for efficient active peptide screening from Inca nut cake protein hydrolysate J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-28 Christoper Caesar Yudho Sutopo, Wei-Ting Hung, Jue-Liang Hsu
Typically, bioactive peptides were uncovered from complex hydrolysates using sequential bioassay-guided fractionation. To increase the efficiency of bioactive peptide screening, a simple and convenient tandem bioassay-guided fractionation based on solid-phase extraction (SPE) was conducted to screen the angiotensin-I-converting enzyme (ACE) inhibitory peptides from the hydrolysate of Inca nut cake
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Updates of the current strategies of labeling for N-glycan analysis J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-27 Yosra Helali, Cédric Delporte
This mini review summarizes the current methods used for screening glycosylation of glycoproteins, with a specific focus on therapeutic proteins and on techniques involving the release of glycans. With the continuous development of biopharmaceuticals, particularly monoclonal antibodies (mAbs), which are glycosylated proteins, monitoring has gained importance in recent decades. Glycosylation of therapeutic
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Utilization of a pH-switchable hydrophilicity solvent for the microextraction of clomipramine from human urine samples J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-23 Maria Terzi, Natalia Manousi, Paraskevas D. Tzanavaras, Constantinos K. Zacharis
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A simple UHPLC-MS/MS method for determination of SET2, a selective antagonist of TRPV2 receptor, in rat plasma samples J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-23 Linda Bartosova, Peter Balis, Vladimir Garaj, Andrej Kovac, Tomas Rajtik, Juraj Piestansky
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Molecular size distribution in pentavalent (A, C, Y, W, X) meningococcal polysaccharide conjugate vaccine by HPSEC-UV-MALS-RI method- a conceivable stability indicating parameter J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-22 Pankaj Sharma, Sameer Kale, Swapnil Phugare, Sumeet Pendharkar, Prasad Shinde, Aarushi Mandhan, Sunil Kumar Goel, Sunil Gairola
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Corrigendum to “Development and validation of a gradient HPLC-UV method for mitragynine following in vitro skin permeation studies” [J. Chromatogr. B 1204 (2022) 123316] J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-21 Yee Shan Sim, Zan Yang Chong, Juzaili Azizi, Choon Fu Goh
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A new combined technology: Macroporous adsorption resin and high speed counter current chromatography for hydroxytyrosol separation from olive leaf enzymatic hydrolysate J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-17 Jiaojiao Yuan, Su Tuo, Xiuhong Shi, Junling Tu
Due to three free hydroxyl groups, hydroxytyrosol (HT) presents strong bioactivity and has broad food and drug application prospects. However, there is no good separation and purification technology. In this study, separation and purification technology of HT from the ethyl acetate extraction of enzymatic hydrolysate from olive leaf (EEEH) was investigated with macroporous adsorption resin (MAR) and
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Measurement of short-chain fatty acids in human plasma by means of fast gas chromatography-mass spectrometry J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-16 Giuseppe Micalizzi, Chiara Buzzanca, Valentina Chiaia, Monica Mondello, Francesco Cacciola, Daniela Caccamo, Luigi Mondello
A rapid and practicable analytical method for the measurement of short-chain fatty acids (SCFAs) in human plasma was developed. The extraction procedure involved the use of acidified water and methyl -butyl ether (MTBE), while the separation and detection of SCFAs, including acetic, propionic, and butyric acids was carried out by using gas chromatography-mass spectrometry (GC–MS) technique. The novelty
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Optimizing and integrating depletion and precipitation methods for plasma proteomics through data-independent acquisition-mass spectrometry J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-16 Yue Zhou, Helong Zheng, Zengqi Tan, Enci Kang, Peng Xue, Xiang Li, Feng Guan
The application of plasma proteomics is a reliable approach for the discovery of biomarkers. However, the utilization of mass spectrometry-based proteomics in plasma encounters limitations due to the presence of high-abundant proteins (HAPs) and the vast dynamic range. To address this issue, we conducted an optimization and integration of depletion and precipitation strategies eliminating interference
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Urinary excretion profile of higenamine in females after oral administration of supplements – Doping scenario J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-15 B. Stojanovic, J. Rasic, M. Andjelkovic, N. Dikic, N. Dragicevic, B. Djordjevic, G. Forsdahl, G. Gmeiner
In 2017, higenamine was added to the (WADA) Prohibited list under group S3: beta-2 agonists and it is banned for athletes both in – and out of competition. Aim of this study was to characterize the urinary excretion profile of higenamine and its metabolite coclaurine after oral administration of multiple doses of higenamine capsules. For this purpose, an administration study including female basketball
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Simultaneous determination of HD56, a novel prodrug, and its active metabolite in cynomolgus monkey plasma using LC-MS/MS for elucidating its pharmacokinetic profile J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-13 Shi Yao, Wenpeng Zhang, Junhai Xiao, Zhiwei Zhang, Lingchao Wang, Hengxiao Ai, Xia Wu, Aibing Chen, Xiaomei Zhuang
An LC-MS/MS method was developed and validated for the simultaneous determination of the carboxylic acid ester precursor HD56 and the active product HD561 in cynomolgus monkey plasma. Then, the pharmacokinetic characteristics of both compounds following single and multiple administrations in cynomolgus monkeys were elucidated. In the method, chromatographic separation was achieved with a C18 reversed-phase
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A validated LC-MS/MS method for determination of six Anti-SARS-CoV-2 drugs in plasma and its application for a pharmacokinetic study in rats J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-07 Zong-liang Xu, Cheng-jian Li, Xian Qian, Hu Duan, Jin Zhou, Qian-qian Zhang, Xin Dong, Liang Zhao
Antiviral treatment for COVID-19 is considered an effective tool in reducing the rate of severe cases and deaths. As of June 2023, a total of six small molecule antiviral drugs have been conditionally approved for marketing by the National Medical Products Administration (NMPA) within China. In this study, a method of HPLC-MS/MS was established and validated for the determination of six small molecule
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Simultaneous quantification of TB-500 and its metabolites in in-vitro experiments and rats by UHPLC-Q-Exactive orbitrap MS/MS and their screening by wound healing activities in-vitro J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-07 Khandoker Asiqur Rahaman, Anca Raluca Muresan, Hophil Min, Junghyun Son, Hyung-Seop Han, Min-Jung Kang, Oh-Seung Kwon
TB-500 (Ac-LKKTETQ), derived from the active site of thymosin β4 (Tβ4), has various biological functions in its unacetylated form, LKKTETQ. These functions include actin binding, dermal wound healing, angiogenesis, and skin repair. The biological effects of TB-500, however, have not been documented. And the analysis of TB-500 and its metabolites have been neither simultaneously quantified nor structurally
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An innovative immunochromatographic assay employing Pt-Pd bimetallic nanoparticles as labels for the detection of foot-and-mouth disease virus serotype O J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-07 Yanyan Sun, Xin Li, Mi Lin, Guang Yang, Huali He, Yanfang Bao, Fengsong Li, Tao Jiang
We created a novel, high sensitivity immunochromatographic assay that allows for clear and precise quantitative analysis by employing innovative bimetallic nanoparticles with peroxide-like activity as markers for the preparation of the test strip. Initially, we synthesized Pt-Pd bimetallic nanoparticles through the reduction of KPtCl and NaPdCl using ascorbic acid (AA) in an ultrasonic water bath.
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Optimization and validation of a UPLC-MS/MS assay for simultaneous quantification of 2,8-dihydroxyadenine, adenine, allopurinol, oxypurinol and febuxostat in human plasma J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-05 Unnur A. Thorsteinsdottir, Hrafnhildur L. Runolfsdottir, Finnur F. Eiriksson, Inger M. Sch. Agustsdottir, Vidar O. Edvardsson, Runolfur Palsson, Margret Thorsteinsdottir
Adenine phosphoribosyltransferase (APRT) deficiency is a rare , hereditary disorder characterized by renal excretion of 2,8-dihydroxyadenine (DHA), leading to kidney stone formation and chronic kidney disease (CKD). Treatment with a xanthine oxidoreductase inhibitor, allopurinol or febuxostat, reduces urinary DHA excretion and slows the progression of CKD. The method currently used for therapeutic
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Verification of chlorine exposure via LC-MS/MS analysis of base hydrolyzed chlorophenols from chlorotyrosine-protein adducts J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-05 Sharmin Sultana, Sarah Christeson, Mohamed Basiouny, Jacqueline Rioux, Livia Veress, Brian A. Logue
Inhalation of chlorine gas, with subsequent hydrolysis in the airway and lungs to form hydrochloric acid (HCl) and hypochlorous acid (HOCl), can cause pulmonary edema (i.e., fluid build-up in the lungs), pulmonary inflammation (with or without infection), respiratory failure, and death. The HOCl produced from chlorine is known to react with tyrosine to form adducts via electrophilic aromatic substitution
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Accelerated development of a SEC-HPLC procedure for purity analysis of monoclonal antibodies using design of experiments J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-05 Terezie Cernosek, Nitin Jain, Matthew Dalphin, Sue Behrens, Peter Wunderli
The complex structure of biopharmaceutical products poses an inherent need for their thorough characterization to ensure product quality, safety, and efficacy. Analytical size exclusion chromatography (SEC) is a widely used technique throughout the development and manufacturing of monoclonal antibodies (mAbs) which quantifies product size variants such as aggregates and fragments. Aggregate and fragment
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Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) methods for the therapeutic drug monitoring of cytotoxic anticancer drugs: An update J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-03 M. Briki, A. Murisier, M. Guidi, C. Seydoux, T. Buclin, C. Marzolini, F. Girardin, Y. Thoma, S. Carrara, E. Choong, L.A. Decosterd
In the era of precision medicine, there is increasing evidence that conventional cytotoxic agents may be suitable candidates for therapeutic drug monitoring (TDM)-guided drug dosage adjustments and patient’s tailored personalization of non-selective chemotherapies. To that end, many liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) assays have been developed for the quantification
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Analysis of free cisplatin in microdialysates and plasma ultrafiltrate by liquid chromatography-tandem mass spectrometry J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-03 A.V. Schmedes, C. Harlev, M. Bue, E.K. Petersen, M.L. Bergmann, L.K. Petersen, M. Stilling
Cisplatin is a potent cytotoxic agent used in the treatment of various malignancies and exerts its antitumor effect through malignant cell DNA damage and apoptosis induction. Evaluation of systemic delivery of cisplatin is important in optimization of cisplatin treatment. However, accurate quantification of systemic cisplatin is challenging due to its various forms in circulation. This study aimed
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Enhancing hyaluronidase enzyme activity: Insights from advancement in bovine and ovine testicular hyaluronidase purification J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-02 Seyed Mohammadhassan Modarressi, Zahra koolivand, Mojdeh Akbari
This essay investigates the use of an affinity resin named Capto lentil lectin for the purification of bovine and ovine testicular hyaluronidase. Hyaluronidase, an enzyme that degrades hyaluronic acid, is used widely in medical fields like dermatology, orthopedics, and ophthalmology. The research highlights the importance of optimizing the purification process to increase enzyme activity and purity
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Developing a detection strategy for ten paralytic shellfish poisonings in urine, combining high-throughput DESI-MS screening and accurate UPLC-QqQ/MS quantification J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-02 Jing Cai, Shiyan Li, Qingcheng Wang, Dan Deng, Shitong Wang, Lijun Ge, Yiwei Cui, Yuejian Shen, Qing Shen
Paralytic shellfish poisoning (PSP) is the most widespread and harmful form of shellfish poisoning with high mortality rate. In this study, a combined desorption electrospray ionization mass spectrometry (DESI-MS) and ultra-performance liquid chromatography triple quadrupole mass spectrometry (UPLC-QqQ/MS) method was established for the detection of PSPs in urine. The method was optimized using a spray
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High performance liquid chromatographic method optimized by Box-Behnken design model to determine caffeine in pharmaceutical preparations and urine samples J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-01 Syed Najmul Hejaz Azmi, Wafa Aqib Nasir Al Rawahi, Arwa Ibrahim Al Yahyai, Asma Ali Al Qasimi, Kothar Saif Al Fuliti, Omayma Said Al Qalhati, Nafisur Rahman, Qamar Uddin Ahmed
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An integrating strategy for systematic profiling of Chinese patent drug’s chemicalome and associated metabolome: Huanghou antidiarrhea dropping pills as a case study J. Chromatogr. B (IF 3.0) Pub Date : 2024-02-01 Li Li, Xuguang Wang, Ruiting Ma, Mingxing Hou
Huanghou antidiarrhea dropping pills (HADP) is an efficient Chinese patent drug that is clinically used to treat diarrhea. However, its functional materials remain unclear due to the characteristics of traditional Chinese medicine, which is a multi-component and multi-target complex system. In this study, we investigated the intrinsic chemical components and combined with metabolism to reveal the functional
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Facial on-line enrichment of glycoproteins by capillary electrophoresis with boronate-functionalized poly(glycidyl methacrylate) microparticles coated column J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-26 Jian Zhang, Yanqing Miao, Hui Jing, Jingwen Wu, Chunye Liu
A facial and rapid method for glycoproteins enrichment by capillary electrophoresis was developed. The 3-aminophenylboronic acid-functionalized poly(glycidyl methacrylate) microparticles (PGMA@APBA) were attached to the capillary inlet (length of ∼1.5 cm) by electrostatic self-assemble action to prepare a partially coated capillary column. The process is simple and reversible, allowing for easy renewal
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A probe of new molecularly imprinted solid-phase extraction coupled with HPLC-DAD and atomic absorption spectrophotometry for quantification of tetracycline HCl, metronidazole and bismuth subcitrate in combination with their official impurities: Application in dosage form and human plasma J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-26 Amal M. Hassan, Khadiga M. Kelani, Maha A. Hegazy, Ahmed H. Nadim, Mahmoud A. Tantawy
The integration of molecular imprinting technique with chromatographic one has a great impact on the assay’s selectivity and sensitivity. Herein, a molecularly imprinted solid-phase extraction associated with high performance liquid chromatography (MISPE-HPLC) was employed for simultaneous determination of the co-formulated drugs; tetracycline hydrochloride (TET) and metronidazole (MET), in plasma
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Ultrasensitive detection of imatinib in human serum using a gold-based paper sensor J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-24 Xiaoqian Jiang, Aihua Qu, Xinxin Xu, Hua Kuang, Liqiang Liu, Chuanlai Xu
Imatinib is the tyrosine kinase inhibitor of choice for the treatment of chronic myeloid leukemia and gastrointestinal stromal tumors. However, imatinib has drawbacks such as drug resistance and significant differences in pharmacokinetics within patients. Therefore, a colloidal gold-based immunochromatographic assay (CG-IA) was developed for measuring and monitoring imatinib in human serum. An imatinib
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A novel method for the accurate quantification of two isomeric mercapturic acids of 1,3-dichlorobenzene in human urine using isotope dilution online-SPE-LC-MS/MS J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-27 T. Schettgen, V. Belov, T. Kraus, P. Ziegler
1,3-dichlorobenzene (1,3-DCB) is an aromatic solvent that might be formed during thermal decomposition of bis(2,4-dichlorobenzoyl)peroxide used as initiator in silicone rubber production with many workers exposed worldwide. During metabolism of 1,3-DCB, two isomeric mercapturic acids can be formed from ring oxidation of 1,3-DCB in the liver, namely 2,4-dichlorophenylmercapturic acid (24CPhMA) and 3
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Screening and identification of antibacterial components in Artemisia argyi essential oil by TLC–direct bioautography combined with comprehensive 2D GC × GC-TOFMS J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-23 Weiyi Wen, Haiping Xiang, Huiyun Qiu, Jianwei Chen, Xuemin Ye, Lin Wu, Zhiqiang Chen, Shengqiang Tong
One of the primary components that contribute to Artemisia argyi 's effectiveness is essential oil, which has an exceptional antibacterial effect that has been well documented. The actual cause of its antibacterial activity and associated mechanism, however, are still not completely understood. For the first time, comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry
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Quantification of mirtazapine in tablets via DNA binding mechanism; development of a new HPLC method J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-26 Ecem Kuzpınar, Abdullah Al Faysal, Pelin Şenel, Taner Erdoğan, Ayşegül Gölcü
Atypical antidepressant mirtazapine (MIR) is mostly prescribed for the management of major depressive disorder. The identification of MIR in pharmaceutical dosage forms was made possible by developing a novel, quick, sensitive high-performance liquid chromatography (HPLC) approach that was verified in accordance with ICH recommendations. In the first part of this study, HPLC investigations were optimized
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Investigation of low-temperature partitioning with dispersive solid-phase extraction for quantification of pesticides in apples followed by electrospray-ionization mobility spectrometry: Comparison with conventional procedure J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-26 Wen Liu, Fei Liu, Anyi Che, Yanjing Chen, Jiayi Cai, Wenjie Liu, Guoxing Jing, Wenshan Li, Jianna Yu
Ion mobility spectrometry (IMS) has a promising application prospect in food surveillance. However, due to the complexity of food matrix and trace levels of pesticide residues, the effective and rapid detection of pesticides by IMS has been a challenge, especially when using electrospray ionization (ESI) as an ion source. In this study, low-temperature partitioning with dispersive solid-phase extraction
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A liquid chromatographic-mass spectrometric procedure for analysis of pentaerythrityl tetranitrate metabolites – Development, validation and application to ovine serum and human plasma samples J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-24 Mariann Städtler, Daniela Wissenbach, Dirk K. Wissenbach, Laura Franke, Jana Pastuschek, Ekkehard Schleussner, Beth Allison, Frank T. Peters, Tanja Groten
Pentaerythrityl tetranitrate (PETN) is an established drug in the treatment of coronary heart disease and heart failure. It is assumed, that the vasodilative and vasoprotective effects of PETN also have a positive impact on pregnant patients with impaired placental perfusion and studies evaluating the effect of PETN in risk pregnancies have been carried out. In the context of these clinical trials
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Determination of sulfadoxine and pyrimethamine in microvolume human plasma using ultra high performance liquid chromatography-tandam mass spectrometry J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-24 Vong Sok, Florence Marzan, Michelle Roh, Kevin Guo, Jenny Legac, Norah Mwebaza, Grant Dorsey, Philip J, Rosenthal, Francesca T. Aweeka, Liusheng Huang
To support the pharmacokinetic study of sulfadoxine (SD) and pyrimethamine (PM) in pregnant women and children, sensitive methods with small sample volume are desirable. Here we report a method to determine SD and PM with microvolume plasma samples: 5 µL plasma samples were cleaned up by protein precipitation with acetonitrile. The deuterated analytes were used as the internal standards. The samples
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On-line PAT based monitoring and control of resin aging in protein A chromatography for COGs reduction J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-20 Anjali Ramakrishna, Anurag S. Rathore
Resin aging is a common occurrence in chromatographic processes and generally influenced by factors such as cleaning procedure and composition of the feed stream. Two major events occur along with protein fouling, one is the loss of protein A ligand and the other is non-specific, irreversible interactions of foulants with resin particles. Both these are responsible for resin aging. As a result, the
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Purification of pineapple bromelain by IMAC chromatography using chlorophyll-activated macroporous matrices J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-22 Márjorie Castro Pinto Porfirio, Jonathan Barbosa Santos, Annie Nolasco Alves, Leandro Soares Santos, Renata Cristina Ferreira Bonomo, Rafael da Costa Ilhéu Fontan
This study investigated the purification of bromelain obtained from pineapple fruit using a new adsorbent for immobilized metal ion affinity chromatography (IMAC), with chlorophyll obtained from plant leaves as a chelating agent. The purification of bromelain was evaluated in batches from the crude extract of pineapple pulp (EXT), and the extract precipitated with 50 % ammonium sulfate (EXT.PR), the
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Measurement of the intracellular active metabolites of thiopurine drugs to evaluate the enzymatic activity of nudix hydrolase 15 in human blood samples J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-18 Hitomi Okamoto, Yoichi Tanaka, Yoshio Shibagaki, Satoshi Kuronuma, Yusuke Miyatani, Satoko Umeda, Emi Mishiro-Sato, Osamu Takeuchi, Seisuke Hattori, Taku Kobayashi, Mitsuru Okuwaki
Thiopurine is metabolized to 6-thio-(deoxy) guanosine triphosphate (6-thio-(d) GTP), which is then incorporated into DNA or RNA and causes cytotoxicity. Nudix hydrolase 15 (NUDT15) reduces the cytotoxic effects of thiopurine by converting 6-thio-(d) GTP to 6-thio-(d) guanosine monophosphate (6-thio-(d) GMP). NUDT15 polymorphisms like the Arg139Cys variant are strongly linked to thiopurine-induced severe
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Monitoring stability indicating impurities and aldehyde content in lipid nanoparticle raw material and formulated drugs J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-18 Robert E. Birdsall, Duanduan Han, Kellen DeLaney, Adam Kowalczyk, Razvan Cojocaru, Matthew Lauber, Jon Le Huray
Lipid nanoparticles (LNPs) are designed to protect and transport sensitive payloads or active pharmaceutical ingredients as part of new therapeutic modalities. As a multi-component particle, a high degree of quality control is necessary to ensure raw materials are free of critical impurities that could adversely impact the drug product. In this study, we demonstrate a reversed phase liquid chromatography
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Establishment and optimization of a high-throughput UPLC-MS/MS method for the simultaneous quantitation of cycloicaritin and its valine carbamate prodrug in rat plasma J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-18 Weiping Wang, Fengxiao Li, Shuo Gan, Jiaqi Fan, Qikun Jiang, Tianhong Zhang
An ultra-performance liquid chromatography-tandem mass spectrometry was developed to assay the concentration for the quantification of cycloicaritin and its carbamate prodrug (3-O-L-valyl carbamate prodrug of cycloicaritin) in the plasma of Sprague-Dawley rats. Analytes were separated on an Acquity UPLC BEH C18 (2.1 × 50 mm, 1.7 μm) after liquid–liquid extraction with methyl tert-butyl ether. Acetonitrile
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Optimized LC-MS/MS method for quantifying insulin degludec and liraglutide in rat plasma and Tissues: Application in pharmacokinetics and biodistribution J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-20 Jeferson Ziebarth, Camila Diedrich, Christiane Schineider Machado, Rubiana Mara Mainardes
We present an ultra-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous detection of insulin degludec (I-Deg) and liraglutide (LIRA) in rat plasma and tissues, characterized by its sensitivity and selectivity. Chromatographic separation was achieved using an Acquity UPLC BEH C18 column, leveraging a mobile phase of acetonitrile and water (both with 0.1 % formic
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Pharmacokinetics and metabolite identification of 23-hydroxybetulinic acid in rats by using liquid chromatography-mass spectrometry method J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-19 Mingzhen Xue, Cheng Yang, Wensi Huang, Yifei He, Chen Yang, Yaru Xue, Yuandong Zheng, Xingxing Diao, Xiachang Wang
23-hydroxybetulinic acid (23-HA), a main bioactive component isolated from Pulsatilla chinensis (Bunge) Regel, exhibits various pharmacological activities, such as antimelanoma, antileukemia, anti-colon cancer, and antihepatotoxicity. Although the main active ingredient anemoside B4 (AB4) from this plant has been well studied, research on its active metabolite 23-HA is limited. In the present study
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A robust and validated LC–MS/MS method for the quantification of ramucirumab in rat and human serum using direct enzymatic digestion without immunoassay J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-19 Wen-si Huang, Wei-qiang Li, Xiong Yu, Ming-zhen Xue, Ya-li Yuan, Chong Chen, Ya-li Wu, Jing-hua Yu, Xing-xing Diao
A new liquid chromatography tandem mass spectrometry (LC–MS/MS) method was established to quantify the anti-gastric cancer fully human monoclonal antibody (ramucirumab) in rat and human serum. The surrogate peptide (GPSVLPLAPSSK) for ramucirumab was generated by trypsin hydrolysis and quantified using the isotopically labeled peptide GPSVLPLAPSSK[13C6, 15N2]ST containing two more amino acids at the
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Hollow-fiber liquid phase microextraction for determination of fluoxetine in human serum by nano-liquid chromatography coupled to high resolution mass spectrometry J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-17 Beatriz Isabella Cestaro, Kelly Cavalcanti Machado, Michel Batista, Bruno José Gonçalves da Silva
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Discovery of bioactive-chemical Q-markers of Acanthopanax sessiliflorus leaves: An integrated strategy of plant metabolomics, fingerprint and spectrum-efficacy relationship research J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-10 Yangyang Liu, Liwei Zhang, Qiuyu Li, Tianyi Xue, Feng Qin, Zhili Xiong
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A LC-MS/MS method for the simultaneous quantitative determination of aldosterone, its precursor 18-hydroxycorticosterone and its metabolite tetrahydroaldosterone in human urine J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-14 Haohang Dong, Bin Hu, Wei Chen, Liping Wang, Huiying Sun, Jianfeng Zhou, Jianchun Chen, Baorong Chen, Zhenxia Du
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Analytical methods for detecting butane, propane, and their metabolites in biological samples: Implications for inhalant abuse detection J. Chromatogr. B (IF 3.0) Pub Date : 2024-01-13 Jihyun Kim, Sanggil Choe, Ilchung Shin, Moonhee Jang, Jaesin Lee, Sang Beom Han
Worldwide, various inhalants are widely abused for recreational purposes, with butane and propane emerging as among the most commonly misused volatile substances, posing a significant risk of sudden death. The rapid elimination and oxidation of these highly volatile compounds upon inhalation necessitate the identification of butane and propane along with their metabolites in biological samples. Hence