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Novel purification platform based on multimodal preparative scale separation of mAb fragments and aggregates J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-13 Rashmi Sharma, Surbhi Gupta, Anurag S. Rathore
Monoclonal antibodies (mAbs) continue to dominate the biopharmaceutical industry. Certain mAbs are prone to fragmentation and clipping and in these cases, adequate removal of these species is critical during manufacturing. Fragments can be generated during fermentation, purification, storage, formulation, and administration. Their addition to the acidic charge-variant of the purified mAb has been reported
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Magnetic polyimide nanocomposite for analysis of parabens in cooking wine by magnetic solid-phase extraction coupled with gas chromatography–mass spectrometry J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-12 Liuxin Wang, Xiaohui Yan, Xianzhong Chen, Yanshuo Li, Dapeng Wu
A magnetic polyimide (PI) nanocomposite has been synthesized by phase inversion of PI and simultaneous encapsulation of FeO nanoparticles. The FeO/PI nanocomposite was characterized by a variety of characterization techniques, including infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, nitrogen adsorption-desorption isotherms, and vibrating sample magnetometry.
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A chromatogram decomposition method and its associated calibration for quantitative analysis J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-12 Can Evren Yarman
We present a new chromatogram decomposition method for Gas Chromatography (GC) which represents a chromatogram as a sum of template functions inspired by the analytic solution of mass balance equation.The proposed method starts by approximating GC response of a single gas by these template functions. Consequently, it utilizes the temporal translation and dilation of this approximate response to approximate
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A functionalized glass fiber as the adsorbent for efficient analysis of endocrine disruptors in aqueous environments J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-11 Yingying Wang, Zhenyu Lu, Yayan Tong, Run Sun, Xue Liu, Na Chen, Mingxia Zhang, Yijun Zhang, Yuping Zhang
Estrogens and bisphenols are typical endocrine disruptors (EDs) that pose a potential hazard to the human body due to their widespread presence in aqueous environments. In this study, a β-cyclodextrin porous crosslinked polymer (β-CD-PCP) was prepared on a glass fiber surface by a nucleophilic substitution reaction. An effective and sensitive solid phase microextraction method using functionalized
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Quantifying Sodium Hypochlorite Content in Hypochlorite-Based Disinfectants via Phase-Conversion Headspace Technique J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-11 Chen-Quan Ni, Wei-Qi Xie
In this work, a novel and efficient approach for sodium hypochlorite analysis is proposed via phase-conversion headspace technique, which is based on the gas chromatography (GC) detection of generated carbon dioxide (CO) from the redox reaction of sodium hypochlorite with sodium oxalate. The data obtained by the proposed method suggest the high detecting precision and accuracy. In addition, the method
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A nitrogenous heterocyclic ring-bonded stationary phase for separating alkaloids in supercritical fluid chromatography J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-11 Chunying Song, Gaowa Jin, Dongping Yu, Zhimou Guo, Xinmiao Liang
A novel silica stationary phase was designed and prepared through thiol-epoxy click chemistry for supercritical fluid chromatography (SFC). The developed stationary phase was characterized by elemental analysis, Fourier transform infrared spectrometry and solid-state C/CP MAS NMR spectroscopy. In order to evaluate the chromatographic performance and retention mechanisms of the prepared column, a variety
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Simultaneous analysis of cannabinoids and terpenes in Cannabis sativa inflorescence using full comprehensive two-dimensional liquid chromatography coupled to smart active modulation J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-11 Sebastián J. Caruso, Agustín Acquaviva, Julian Lemus Müller, Cecilia B. Castells
Nowadays, the higher peak capacity achievable by comprehensive two-dimensional liquid chromatography (LC×LC) for the analysis of vegetal samples is well-recognized. In addition, numerous compounds may be present in very different amounts. Cannabinoids and terpenes represent the main components of inflorescence samples, whose quantities are relevant for many application purposes. The analyses of both
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Magnetic solid-phase extraction based on restricted-access molecularly imprinted polymers for ultrarapid determination of ractopamine residues from food samples by capillary electrophoresis J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-10 Daniel Ferreira Vidal, Bruna Carneiro Pires, Marcella Matos Cordeiro Borges, Hanna Leijoto de Oliveira, Camilla Fonseca Silva, Keyller Bastos Borges
An ultrafast, efficient, and eco-friendly method combining magnetic solid phase extraction and capillary electrophoresis with diode array detection have been developed to determine ractopamine residues in food samples. A restricted access material based on magnetic and mesoporous molecularly imprinted polymer has been properly synthesized and characterized, demonstrating excellent selectivity and high
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A review of analytical parameters in ‘rapid’ liquid chromatographic methods for bioanalysis: can we do better? J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-08 K Lawlor, J Clausen, A Johnston, A Edge, K Wolff, E Castrignanò, L Couchman
Rapid bioanalysis is beneficial to many applications. However, how ‘rapid’ a method is, or could be, is often an unanswered question. In this statistical review, the authors have assessed multiple pre-analytical (i.e. sample preparation), and analytical method parameters specifically for liquid chromatography to assist researchers in developing and validating ‘rapid’ bioanalytical methods. We restricted
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Quantifying chemical correlations between fruits and processed fruit products: A non-targeted analysis approach J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-08 Ren-Qi Wang, Ye Geng, Ni-Jing Zhou, Juan-Na Song, Huai-Dong Yu, Yan-Ru Liu, Zheng-Gang Yue, Ruo-Qi Li, Qi Chang, Xiu-Juan Xu, Chun-Qiang Yang, Jian-Kang Wang, Zhi-Shu Tang
Juices and beverages are produced by industry for long-distance distribution and shelf-stability, providing valuable nutrients. However, their nutritional value is often underestimated due to insufficient analytical methods. We have employed non-targeted analysis through a standardized analytical protocol, taking advantage of Data Independent Acquisition (DIA) technique and a novel Chromatographic
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Internal multiple interactions-adsorption and external zwitterionic polymer-exclusion of restricted access materials as adsorbent for offline and online extraction of neonicotinoid pesticides in Goji samples J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-07 Chunmiao Bo, Yinhai Li, Bin Liu, Xiaofan Tang, Shengwei Guo, Guijuan Ma, Yan Li, Weilong Zhao
A method based on novel restricted access materials (RAMs) for the determination of neonicotinoid pesticides in Goji samples using offline and online solid phase extraction (SPE) coupled with high-performance liquid chromatography (LC). RAMs were synthesized using poly(chloromethylstyrene--divinylbenzene) (P) microspheres as substrate, styrene (St) and n-vinylpyrrolidone (NVP) were first copolymerized
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Automatic procedure for modelling, calibration, and optimization of a three-component chromatographic separation J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-07 Daniel Espinoza, Simon Tallvod, Niklas Andersson, Bernt Nilsson
The current landscape of biopharmaceutical production necessitates an ever-growing set of tools to meet the demands for shorter development times and lower production costs. One path towards meeting these demands is the implementation of digital tools in the development stages. Mathematical modelling of process chromatography, one of the key unit operations in the biopharmaceutical downstream process
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Discrimination of plastic waste pyrolysis oil feedstocks using supercritical fluid chromatography J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-07 Alexander S. Kaplitz, Shane Marshall, Niray Bhakta, Sadid Morshed, Jean-François Borny, Kevin A. Schug
Advanced chemical recycling techniques provide new avenues for handling and recycling mixed plastic waste; pyrolysis is a prominent approach involving heating plastic waste in an oxygen-free environment to create pyrolysis oils. Pyrolysis oils must be thoroughly characterized before being refined into fuels and chemical feedstocks. Here, a method based on supercritical fluid chromatography with ultraviolet
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Investigating cationic and zwitterionic successive multiple ionic-polymer layer coatings for protein separation by capillary electrophoresis J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-06 Laura Dhellemmes, Laurent Leclercq, Henry Frick, Alisa Höchsmann, Norbert Schaschke, Christian Neusüß, Hervé Cottet
Successive multiple ionic-polymer layers (SMILs) have long since proved their worth in capillary electrophoresis as they ensure stable electroosmotic flow (EOF) and relatively high separation efficiency. Recently, we demonstrated that plotting the plate height () against the solute migration velocity () enabled a reliable quantitative evaluation of the coating performances in terms of separation efficiency
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A rapid one-step affinity purification of C-phycocyanin from Spirulina platensis J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-06 Ke Shi, Wei Wang, Jingjing Sun, Chengcheng Jiang, Jianhua Hao
The high-purity phycocyanin has a high commercial value. Most current purification methods of C-phycocyanin involve multiple steps, which are complicated and time-consuming. To solve the problem, this research was studied, and an efficient affinity chromatography purification for C-phycocyanin from was developed. Through molecular docking simulation, virtual screening of ligands was performed, and
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Adsorption removal of cationic dyes from wastewater using the corn straw modified with diethylenetriaminepentacetic acid ligand J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-06 Hongbin Hu, Lang Zhao, Lu Yao, Min He, Yuwei Lv, Rong Li
Taking the thiazide cationic dye methylene blue (MB), triphenylmethane cationic dye crystal violet (CV), monoazo cationic dye cationic red 46 (R-46), and polycarboxycyanine cationic dye cationic rosé FG (P-FG) as the research objects, the adsorption behaviors of a self-made corn straw modified adsorbent HQ-DTPA-I for the dyes were investigated in depth. Under optimized conditions, HQ-DTPA-I can quickly
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One-pot preparation of magnetic composite containing boronic acid groups and aminated multwalled carbon nanotubes for the speciation of Se(IV) and Se(VI) in water and milk samples by combination with chromatographic quantification J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-05 Xin Meng, Yueyue Zhang, Xiaochong Song, Qing Luo, Xiaojia Huang
Speciation of Se(IV) and Se(VI) is essential due to their significant differences in reactivity, toxicity and bioavailability. Efficient extraction is the pivotal step in the quantification of inorganic selenium species. In this work, a new magnetic nano-composite (MNC) containing boronic acid group and aminated multwalled carbon nanotubes was facilely fabricated by means of one-pot hydrothermal strategy
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Preparation and evaluation of two chiral stationary phases based on imidazolyl-functionalized bromoethoxy pillar[5]arene-bonded silica J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-05 Tong Li, Hui Li, Jia Chen, Yongliang Yu, Shuai Chen, Jianhua Wang, Hongdeng Qiu
Chiral pillar[5]arene-based mesoporous silica, an emerging class of chiral structure, possesses excellent characteristics such as abundant chiral active sites, encapsulated cavity and excellent chiral modification, which make them a promising candidate as new chiral stationary phases (CSPs) in enantioseparation. In this study, two imidazole-containing ()-1-(4-phenyl-1H-imidazol-2-yl)ethanamine and
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Determination of lipophilic marine biotoxins (azaspiracids, brevetoxins, and okadaic acid group) and domoic acid in mussels by solid-phase extraction and reversed-phase liquid chromatography with tandem mass spectrometry J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-05 Naoki Ochi, Toshiyuki Suzuki
An accurate and efficient method was developed for the determination of azaspiracid shellfish toxins (azaspiracids-1, -2, and -3), neurotoxic shellfish toxins (brevetoxins-2 and -3), diarrhetic shellfish toxins (okadaic acid and dinophysistoxins-1 and -2), and the amnesic shellfish toxin (domoic acid) in mussels (). Lipophilic marine biotoxins (azaspiracids, brevetoxins, and okadaic acid group) were
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Optimization of elution conditions and comparison of emerging biocompatible columns on the resolving power and detection sensitivity of oligonucleotides by ion-pairing reversed-phase liquid chromatography mass spectrometry J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-05 Quang-Dong Bui, Tiny Deschrijver, Bart Noten, Willy Verluyten, Nico Vervoort, Sebastiaan Eeltink
A generic performance comparison strategy has been developed to evaluate the impact of mobile-phase additives (ion-pairing agent / counter ion systems), distinct stationary phases on resulting resolving power, and MS detectability of oligonucleotides and their critical impurities in gradient IP-RPLC. Stationary-phase considerations included particle type (core-shell fully porous particles), particle
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Preparation of magnetic amphiphilic resin microspheres via the one-step polymerization method and extraction of four glucocorticoids for HPLC–MS analysis J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-05 Zibo Zhou, Liang He, Tianyi Wang, Hongzhen Tang, Suzi Qin, Xueyan Nan, Wenbo Cheng, Haibo He, Pengli Bai
Amphiphilic materials can be used for sample preparation of chromatography or mass spectrometry. Amphiphilic materials with magnetic properties in combination with magnetic suction devices allow for automated sample preparation. However, conventional synthesis methods are cumbersome and not suitable for the mass production of the material. In this study, a micro-suspension polymerization method was
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Controlled synthesis of sulfhydryl-dendritic mesoporous silica nanospheres for ultrafast extraction and sensitive analysis of organochlorine herbicides containing amide groups J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-05 Rui Shi, Yipeng Huang, Guihua Ruan, Zhengyi Chen, Yanqun Yang, Zhuqiang Wu
The distinctive morphology of dendritic mesoporous silica nanoparticles (DMSN) has recently attracted considerable attention in scientific community. However, synthesis of DMSN with well-defined structure and uniform size for ultrafast extraction of trace herbicide residues from environmental and food samples remains to be a compelling challenge. In this study, sulfhydryl functionalized dendritic mesoporous
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Revealing receptor-ligand interactions of atypical antipsychotic drugs and screening anti-schizophrenia ingredients in Magnolia officinalis based on 5-HTR2A-SNAP-Tag/CMC and DRD2-SNAP-Tag/CMC models J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-03 Chunlei Gao, Tongpei Zhou, Sihan Liu, Chenyang Miao, Jingting Feng, Yifan Ding, Yanni Lv
Schizophrenia is a serious mental illness with unknown etiology, and shows increasing incidence and high lifetime prevalence rate. The main receptors related to the disease are DRD2 and 5-HTR2A. Thus, a comprehensive understanding of the interaction mode between antipsychotic drugs with relevant receptors is very important for developing more effective drugs. 5-HTR2A-SNAP-Tag/CMC and DRD2-SNAP-Tag/CMC
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Novel approach over template molecule elimination in molecularly imprinted polymers by using heat activated persulfate J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-01 Juan I. Calbanese, Mario D. Contin, Pablo R. Bonelli, Ana L. Cukierman, Valeria P. Tripodi
This study proposes a new alternative for template removal from molecularly imprinted polymers by heat activated persulfate. It is known that trace amounts of template molecule remains in the polymer network after extraction by current methodologies leading to bleeding and incomplete removal of template which could compromise final determination of target analytes especially in trace analysis. A previously
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Design and evaluation of semicarbazide-embeddedd stationary phases for liquid chromatography J. Chromatogr. A (IF 4.1) Pub Date : 2024-03-01 Yujie Zhang, Mingyang Zhao, Chuanbo Fu, Shouyong Zhou, Haifeng Han, Mingliang Zhang
Semicarbazide, as a derivative of urea, constitutes a great variety of functional molecules for different needs. Herein, novel stationary phases with an incorporated semicarbazide group were proposed. Using aliphatic (docosanoyl, C22) and aromatic (benzoyl, Bz) hydrazides, the semicarbazide-embedded ligands were synthesized before chemical modification of the silica gel, allowing for an accurate interpretation
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Separation of carbohydrates using dynamically adsorbed borate stationary phase for hydrophilic interaction liquid chromatography J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-29 Yang Zhao, Yufang Ming, Yang Yang, Changyu Cai, Yujie Bi, Qing Fu, Yanxiong Ke
In this work, a chromatographic method for the separation of carbohydrates was proposed. Tris-(hydroxymethyl)-amine (TRIS) functionalized silica-based hydrophilic interaction liquid chromatography (HILIC) stationary was synthesized. The dynamically absorbed borate layer is generated by using borate buffer as a polar modifier due to the complexation of borate with TRIS ligand in the stationary phase
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Single injection LC-MS/MS analytical method for the quantification of diverse families of micropollutants, including PFAS and organotins, in Gammarus fossarum J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-29 Mathilde Duny, Aurélie Cortéjade, Laure Wiest, Mickael Nicolas, Emmanuelle Vulliet
Since the last decades, light has been shed on the pollution of aquatic ecosystems. Considering apolar compounds in water, analysis of the dissolved phase is not sufficient due to their possible bioaccumulation. Additional analysis of sediments, biota, or sentinel species is necessary. Among sentinel species, is a small shrimp of 30 mg that lives naturally in the river. Its ability to bioaccumulate
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Enantiomeric analysis of γ(δ)-lactones by reversed phase high performance liquid chromatography using amylose tris(5-chloro-2-methylphenylcarbamate) as stationary phase J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-29 Junchao Zhu, Songlin Chen, Beibei Zhu, Chenlei Ma, Huiyun Qiu, Lang Chen, Shengqiang Tong
A Chiralpak AY-3R column was investigated for analytical enantiomeric separation of twelve racemic ()-lactones using reversed phase high performance liquid chromatography. Main influence factors, including organic modifier, flow rate and column temperature, were optimized. Five kinds of ()-lactones were successfully enantioseparated using the established method: nonanolactone, decalactone, -undecalactone
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Recent advance on microextraction sampling technologies for bioanalysis J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-28 Na Li, Zhuomin Zhang, Gongke Li
The contents of target substances in biological samples are usually at low concentration levels, and the matrix of biological samples is usually complex. Sample preparation is considered a very critical step in bioanalysis. At present, the utilization of microextraction sampling technology has gained considerable prevalence in the realm of biological analysis. The key developments in this field focus
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Deep reinforcement learning for the direct optimization of gradient separations in liquid chromatography J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-28 Alexander Kensert, Pieter Libin, Gert Desmet, Deirdre Cabooter
While Reinforcement Learning (RL) has already proven successful in performing complex tasks, such as controlling large-scale epidemics, mitigating influenza and playing computer games beyond expert level, it is currently largely unexplored in the field of separation sciences. This paper therefore aims to introduce RL, specifically proximal policy optimization (PPO), in liquid chromatography, and evaluate
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Capillary electrophoresis using triple layer modified capillary facilitating salivary ion analyses: Application to search for potential stress markers induced by cold pressure test J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-28 Hironori Myochin, Noriyasu Ohshima, Takashi Izumi, Tatsuya Hisajima, Romanas Chaleckis, Masanobu Mori
In this study, we introduce a novel approach for the analysis of salivary ions using capillary electrophoresis (CE) with a triple-layer coated capillary. The capillary is sequentially coated with cationic silylating reagents, poly(vinylsulfonate), and polybrene to form a custom designed surface that effectively inhibits adsorption of protein matrix on the capillary inner wall and allows for reproducible
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Differentiation of isomeric chalcone and dihydroflavone using liquid chromatography coupled with hydrogen-deuterium exchange tandem mass spectrometry (HDX-MS/MS): An application for flavonoids-focused characterization of Snow chrysanthemum J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-27 Jingjing Shi, Ke Zhang, Ting Li, Lijuan Wu, Yang Yang, Yuan Zhang, Pengfei Tu, Wenjing Liu, Yuelin Song
Although the co-occurrences of isomeric chalcones and dihydroflavones widely appear in medicinal plants, the differentiation of such isomerism seldom succeeds using MS/MS, attributing to totally identical MS/MS spectra. Here, efforts were paid to pursue an eligible tool allowing to address the technical challenge. Being inspired by that one more proton signal is observed in H NMR spectrum of isoliquiritigenin
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Preparation and investigation of two-component silica-modified hydrophobic layer for minimizing retention loss of reversed-phase chromatographic columns using fully aqueous mobile phases J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-27 Fabrice Gritti, Nicole Lawrence, Jessica Field
Chromatographers often employ fully aqueous mobile phases to retain highly polar compounds in reversed-phase liquid chromatography (RPLC). However, when the flow rate is interrupted, either accidentally or intentionally, a substantial loss in retention occurs due to the spontaneous dewetting of water from the hydrophobic surface of conventional RPLC-C particles. Previous studies have shown that maintaining
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Preparation of thiol-free amino acid plasma matrix for quantification of thiol amino acids and their oxidized forms in human plasma by UPLC-MS/MS J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-27 Ting Tao, Yuyu Cao, Liping Zhang, Dayi Xu, Kaiyuan Pang, Yanli Li, Xiaobao Wei, Zengxian Sun
Thiol amino acids, with great physiological significance, are unstable, and have small molecular weights, as well as very low endogenous concentrations. Therefore, to quantitatively and directly analyze them using liquid chromatography-tandem mass spectrometry is difficult. To overcome these problems, we aimed to prepare a thiol-free amino acid plasm matrix as blank sample to reduce the background
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Mixed-mode size-exclusion silica resin for polishing human antibodies in flow-through mode J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-27 Jacob P. LeBarre, Wenning Chu, Scott H. Altern, Andrew J. Kocot, Dipendra Bhandari, Eduardo Barbieri, Jae Sly, Michael Crapanzano, Steven M. Cramer, Michael Phillips, David Roush, Ruben Carbonell, Cristiana Boi, Stefano Menegatti
The polishing step in the downstream processing of therapeutic antibodies removes residual impurities from Protein A eluates. Among the various classes of impurities, antibody fragments are especially challenging to remove due to the broad biomolecular diversity generated by a multitude of fragmentation patterns. The current approach to fragment removal relies on ion exchange or mixed-mode adsorbents
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High throughput multidimensional liquid chromatography approach for online protein removal and characterization of polysorbates and poloxamer in monoclonal antibody formulations J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-27 Maksymilian M. Zegota, Georg Schuster, Mauro De Pra, Tibor Müllner, Tim Menzen, Frank Steiner, Andrea Hawe
The majority of commercially available monoclonal antibody (mAb) formulations are stabilized with one of three non-ionic surfactants: polysorbate 20 (PS20), polysorbate 80 (PS80), or poloxamer 188 (P188). All three surfactants are susceptible to degradation, which can result in functionality loss and subsequent protein aggregation or free fatty acid particle formation. Consequently, quantitative, and
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Specific fractionation of ginsenosides based on activated carbon fibers and online fast screening of ginseng extract by mass spectrometry J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-27 Meiyu Cui, Zakia Fatima, Zhao Wang, Yang Lei, Xiangai Zhao, Mingshi Jin, Lu Liu, Chunyu Yu, Meihui Tong, Donghao Li
Ginseng is beneficial in the prevention of many diseases and provides benefits for proper growth and development owing to the presence of various useful bioactive substances of diverse chemical heterogeneity (e.g., triterpenoid saponins, polysaccharides, volatile oils, and amino acids). As a result, understanding the therapeutic advantages of ginseng requires an in-depth compositional evaluation employing
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Temperature-responsive comprehensive two-dimensional liquid chromatography coupled to high resolution mass spectrometry for the elucidation of the oxidative degradation processes of chemicals of environmental concern J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-24 Elena Bandini, Kristina Wicht, Maike Felipe Santos Barbetta, Hamed Eghbali, Frédéric Lynen
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Silver nanoparticle-functionalized melamine-formaldehyde aerogel for online in-tube solid-phase microextraction of polycyclic aromatic hydrocarbons followed by HPLC-DAD analysis J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-24 Xubo Xin, Chunying Li, Min Sun, Wenjuan Guo, Juanjuan Feng
Based on the π-metal interaction between silver nanoparticles (AgNPs) and aromatic compounds, AgNPs were in-situ grown to melamine-formaldehyde (MF) aerogel for improving the extraction performance to polycyclic aromatic hydrocarbons (PAHs). The AgNPs/MF aerogel was regulated through varing the concentration of reactants, and characterized by scanning electron microscopy, X-ray photoelectron spectroscopy
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Chromatographic separation by RPLC-ESI-MS of all hydroxyproline isomers for the characterization of collagens from different sources J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-24 Martina Lioi, Sara Tengattini, Roberto Gotti, Francesca Bagatin, Stefano Galliani, Gabriella Massolini, Simona Daly, Caterina Temporini
During collagen biosynthesis, proline is post-translationally converted to hydroxyproline by specific enzymes. This amino acid, unique to collagen, plays a crucial role in stabilizing the collagen triple helix structure and could serve as an important biomarker for collagen content and quality analysis. Hydroxyproline has four isomers, depending on whether proline is hydroxylated at position 4 or 3
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Impact of diluents on the calibration of direct dynamic thermal desorption prior to gas chromatography J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-24 Wenping Huang, Kris Wolfs, Ana Modrić, Ann Van Schepdael, Erwin Adams
A thermal desorber (TD) can be used in different ways to introduce samples in a gas chromatographic (GC) system. Besides its conventional use where the collected analytes are released from the sorbent in the sample tube, direct dynamic desorption (DDD) is an interesting option where a solid sample is put directly in the TD tube. However, since no sorbent is used for the sample, proper calibration is
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Relative quantitation of chiral thiol compounds labeled based on isotope novel mass spectrometry probes: Monitoring of the dynamic changes of chiral thiol compounds in human urine during normal, exercise, and rest recovery states J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-20 Chenglong Yu, Yuxuan Li, Xi-Ling Li, Chengqiang Han, Jing Li, Ri Jin, Jun Zhe Min
Monitoring changes in the content of chiral thiol compounds in the human body is crucial for the early diagnosis of oxidative stress-related diseases and the exploration of their pathogenesis. To address this, we synthesized a novel isotope mass spectrometry (MS) probe, denoted as ()-(5-(3-isothiocyanato (C) pyrrolidin-1-yl)-5-oxopentyl) triphenylphosphonium (NCS-OTPP), with triphenylphosphine as its
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Synthesis of MIL-88(Fe) coordinated to carboxymethyl cellulose fibers nanocomposite for dispersive solid phase microextraction of acetanilide herbicides from cereal and agricultural soil samples J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-20 Zolfaghar Aladaghlo, Siamak Javanbakht, Ali Sahragard, Alireza Fakhari
In this study, MIL-88(Fe) coordinated to carboxymethyl cellulose fibers was successfully synthesized, characterized, and utilized as a nanocomposite for the dispersive solid phase microextraction of butachlor and acetochlor. These analytes served as representative analytes for acetanilide herbicides (AHs) present in real samples. Effective parameters on the extraction efficiency were investigated to
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Enhancing LOD determination in gas chromatography: Validating the Hubaux-Vos method for gas concentration measurement J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-20 Hyeong Rae Kim, Sunwoo Kim, Jinsang Jung, Hwan Lee, Keon Ho, Byeongmoon Kim, Sanghyub Oh
The limit of detection (LOD) is a crucial measure in analytical methods, representing the smallest amount of a substance that can be distinguished from background noise. In the realm of gas chromatography (GC), however, determining LOD can be quite subjective, leading to significant variability among researchers. In this study, we validate the Hubaux-Vos method, an International Standards Organization(ISO)-approved
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Evaluation of size-exclusion chromatography, multi-angle light scattering detection and mass photometry for the characterization of mRNA J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-20 Jelle De Vos, Kris Morreel, Piotr Alvarez, Helena Vanluchene, Robbe Vankeirsbilck, Pat Sandra, Koen Sandra
The recent approval of messenger ribonucleic acid (mRNA) as vaccine to combat the COVID-19 pandemic has been a scientific turning point. Today, the applicability of mRNA is being demonstrated beyond infectious diseases, for example in cancer immunotherapy, protein replacement therapy and gene editing. mRNA is produced by in vitro transcription (IVT) from a linear DNA template and modified at the 3′
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An HPLC-ESI-QTOF method to analyze polar heteroatomic species in aviation turbine fuel via hydrophilic interaction chromatography J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-19 Amanda M. Arts, Paul J. Wrzesinski, Zachary J. West
Aviation turbine fuel is a complex mixture of thousands of compounds. An analytical method using hydrophilic interaction liquid chromatography (HILIC) coupled with electrospray ionization and quadrupole time-of-flight mass spectrometry (ESI-QTOF) was developed for the identification of heteroatomic, polar compounds in aviation turbine fuel. Although compounds containing oxygen, nitrogen, and sulfur
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Separation, purification and structural characterization of marine oligosaccharides: A comprehensive and systematic review of chromatographic methods J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-19 Limin Ning, Benwei Zhu, Zhong Yao
Marine oligosaccharides have now been applied in a wide range of industry due to various kinds of physiological activities. However, the oligosaccharides with different polymeric degrees (Dps) differed in physiological activities and applicable fields. So it is promising and essential to separate, purify and structurally characterize these oligosaccharides for understanding their structure-function
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Facile synthesis of Fe3+ immobilized magnetic polydopamine-polyethyleneimine composites for phosphopeptide enrichment J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-18 Xinzhuang Wei, Xue Wen, Haijiao Zheng, Yang Zhang, Qiong Jia
As one of the most common post-translational modification of proteins, protein phosphorylation plays a vital role in many physiological processes. The enrichment of phosphopeptides is highly important before the mass spectrometry detection since phosphopeptides are susceptible to interferences from high-abundance non-phosphopeptides. In this study, we designed a novel magnetic composite (FeO@PDA-PEI-Fe)
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Chiral recognition without π−π-interactions: Highly efficient chiral strong cation exchangers lacking an aromatic unit in the molecular structure J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-16 Magdaléna Labíková, Jiří Svoboda, Jiří Tůma, Wolfgang Lindner, Michal Kohout
Current state-of-the-art chiral stationary phases (CSPs) enable chiral resolution of almost any racemic mixture of choice. The exceptions represent ionizable and ionized substances that fail at any attempts to resolve on commercially available CSPs. These compounds, however, can be efficiently separated on chiral ion exchangers. Commercially available alkaloids-based chiral weak ion-exchangers are
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Efficient actinide sequestration with ionic liquid-based extraction chromatography resins containing Aza-crown ether functionalized diglycolamides J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-16 Piyali Banerjee, Seraj A. Ansari, Thichur P. Valsala, Raj B. Bhatt, Richard J.M. Egberink, Willem Verboom, Prasanta K. Mohapatra
Two new extraction chromatographic resins (ECRs) were prepared by impregnating two exotic diglycolamide (DGA) ligands (having three or four DGA moieties tethered to aza-crown ether scaffolds) dissolved in an ionic liquid onto an inert solid support. A room temperature ionic liquid (RTIL) was used for enhancing the performance of the ECRs. The ECR containing triaza-9-crown-3 functionalized with three
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Online coupling of liquid chromatography and two-dimensional diffusion ordered spectroscopy for the analysis of oligostyrenes J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-16 Bastian Grabe, Wolf Hiller
The aim of this study was to introduce a powerful coupling of Liquid Adsorption Chromatography (LAC) and Diffusion-Ordered Spectroscopy (DOSY) for comprehensive structure analysis. This new hyphenation approach facilitated the simultaneous separation of a polymer mixture and the determination of molar masses within a single 3D experiment. The online coupling of High-Performance Liquid Chromatography
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Extraction of Volatile Organic Compounds Liberated Upon Filament Extrusion by 3D Pen and its Comparison with a Desktop 3D Printer Using Solid-Phase Microextraction Fiber and Arrow J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-14 Bhawana Thapa, David S. Bell, Jared L. Anderson
Desktop 3D printers that operate by the fused deposition modeling (FDM) mechanism are known to release numerous hazardous volatile organic compounds (VOCs) during printing, including some with potential carcinogenic effects. Operating in a similar manner to FDM 3D printers, 3D pens have gained popularity recently from their ability to allow users to effortlessly draw in the air or create various 3D
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Development of a three-dimensional HPLC system for the determination of serine, threonine and allo-threonine enantiomers in the plasma of patients with chronic kidney disease J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-14 Mai Oyaide, Chiharu Ishii, Takeyuki Akita, Tomonori Kimura, Shinsuke Sakai, Masayuki Mizui, Masashi Mita, Tomomi Ide, Yoshitaka Isaka, Kenji Hamase
A highly-selective three-dimensional high-performance liquid chromatographic (3D-HPLC) system was developed for the determination of serine (Ser), threonine (Thr) and -threonine (aThr) enantiomers in human plasma to screen the new biomarker of chronic kidney disease (CKD). -Ser has been reported to be the candidate biomarker of CKD, however, multiple biomarkers are still required. Therefore, Ser analogs
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Revisiting column selectivity choices in ultra-high performance liquid chromatography–Using multidimensional analytical Design Spaces to identify column equivalency J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-14 Arnold Zöldhegyi, Krisztián Horváth, Róbert Kormány
Current guides and column selection system (CSS) platforms can provide some helpful insights with regard to the selection of alternative phases. Their practical reliability however, can also turn out to be questionable, especially considering the lack of detailed specifics, such as a clear definition of points of equivalence—appropriate running conditions under which the given analytical mixture can
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Titania-based fabric phase sorptive extraction approach for the determination of antiepileptic drugs, levetiracetam and lamotrigine in urine samples using high-performance liquid chromatography-photo diode array detection J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-14 Songül Ulusoy, Halil İbrahim Ulusoy, Marcello Locatelli, Abuzar Kabir
A new fabric phase sorptive extraction (FPSE) based separation and enrichment method was developed for sensitive determination of two antiepileptic drug molecules, Levetiracetam (LEV) and Lamotrigine (LTG). The analysis of these drug molecules was performed with high-performance liquid chromatography equipped with photodiode array detector (HPLC-PDA) after FPSE. HPLC analysis was carried out by using
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An active derivatization detection method for inline monitoring the isolation of carbohydrates by preparative liquid chromatography J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-14 Zhen Yao, Kehan Zhu, Tianyi Gu, Oliver J. Schmitz, Duxin Li
Polysaccharides have unique physio-chemical properties and various biological functions and have rapidly expanded interest over the last two decades. The purification of polysaccharides and their degraded oligosaccharides is challenging because carbohydrates have no chromophore and need a proper detector to monitor the chromatographic elution process. This study proposed an active derivatization detection
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A quantitative chemomics strategy for the comprehensive comparison of Murraya paniculata and M. exotica using liquid chromatography coupled with mass spectrometry J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-12 Haizhen Liang, Shuo Yuan, Xiaoli Ma, Qingqing Song, Yuelin Song, Pengfei Tu, Yong Jiang
Murrayae Folium et Cacumen (MFC) is a traditional Chinese medicine (TCM) derived from two plant species, L. and (L.) Jack, as recorded in the Chinese Pharmacopoeia. However, there is no research available on the comprehensive analysis and comparison of the chemical constituents of these two species. In the present study, an integrated LC-MS-based quantitative metabolome strategy was proposed to conduct
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In-line buffer exchange in the coupling of Protein A chromatography with weak cation exchange chromatography for the determination of charge variants of immunoglobulin G derived from chinese hamster ovary cell cultures J. Chromatogr. A (IF 4.1) Pub Date : 2024-02-11 Sarah K. Wysor, Benjamin F Synoground, Sarah W. Harcum, R. Kenneth Marcus
Immunoglobulin G (IgG) is the most common monoclonal antibody (mAb) grown for therapeutic applications. While IgG is often selectively isolated from cell lines using protein A (ProA) chromatography, this is only a stepping stone for complete characterization. Further classification can be obtained from weak cation exchange chromatography (WCX) to determine IgG charge variant distributions. The charge