Phosphorylation has an incredible impact on the biological behavior of proteins, altering everything from intrinsic activity to cellular localization and complex formation. It is no surprise then that this post-translational modification has been the subject of intense study and that, with the advent of faster, more accurate instrumentation, the number of large-scale mass spectrometry-based phosphoproteomic studies has swelled over the past decade. Recent developments in sample preparation, phosphorylation enrichment, quantification, and data analysis strategies permit both targeted and ultra-deep phosphoproteome profiling, but challenges remain in pinpointing biologically relevant phosphorylation events. We describe here technological advances that have facilitated phosphoproteomic analysis of cells, tissues, and biofluids and note applications to neuropathologies in which the phosphorylation machinery may be dysregulated, much as it is in cancer.

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磷酸蛋白质组学及其在神经系统疾病中的应用的最新进展

磷酸化对蛋白质的生物学行为产生了不可思议的影响，从内在活性到细胞定位和复合物形成都发生了改变。毫不奇怪，这种翻译后修饰一直是深入研究的主题，并且随着更快，更准确的仪器的出现，基于质谱的大规模磷酸化蛋白质组学研究的数量在过去十年中激增。样品制备，磷酸化富集，定量和数据分析策略的最新进展允许靶向和超深度磷酸化蛋白质组分析，但在查明生物学相关的磷酸化事件方面仍然存在挑战。我们在这里描述了促进细胞，组织，细胞和组织的磷酸化蛋白质组学分析的技术进步，