The reliable detection and monitoring of microRNAs (miRNAs) are of great significance for gaining a better understanding of the functions of miRNAs in a wide range of biological processes. In this work, a competitive DNA microarray-based resonance light scattering (RLS) assay has been developed for the multiplexed detection of miRNAs with relatively high sensitivity and selectivity. After one-step competition hybridization reactions of miRNAs and multiple single strand DNA (ssDNA) conjugated gold nanoparticles (ssDNAs_n@GNPs) with immobilized ssDNA probes on the dendrimer-modified slide, the captured ssDNAs_n@GNPs are further enlarged through silver deposition. The silver enlarged ssDNAs_n@GNPs can generate strong RLS under white light excitation. The simultaneous detection of multiple miRNAs can be easily achieved by monitoring the RLS signal changes of microarrays. In the proof of concept experiment, eight miRNA let-7 family members are detected with high specificity down to 0.2 pM, 0.8 pM, 0.2 pM, 0.2 pM, 0.2 pM, 0.8 pM, 0.8 pM, and 0.8 pM for miRNA let-7a to 7g, and 7i, respectively. Furthermore, the expression levels of eight miRNA let-7 family members in the total RNA extracts from five cell lines have been evaluated, and satisfactory results are obtained.

中文翻译：

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通过基于竞争性DNA微阵列的共振光散射测定法对microRNA进行多重检测

microRNA（miRNA）的可靠检测和监控对于更好地了解miRNA在广泛的生物学过程中的功能具有重要意义。在这项工作中，已经开发出一种竞争性的基于DNA微阵列的共振光散射（RLS）分析方法，用于以相对较高的灵敏度和选择性对miRNA进行多重检测。在将miRNA与多个单链DNA（ssDNA）共轭的金纳米颗粒（ssDNAs _n @GNPs）与固定的ssDNA探针在树状聚合物修饰的载玻片上进行一步竞争杂交反应后，通过银沉积进一步扩大了捕获的ssDNAs _n @GNPs。银扩大的ssDNAs _Ñ@GNP可以在白光激发下产生强大的RLS。通过监测微阵列的RLS信号变化，可以轻松同时检测多个miRNA。在概念验证实验中，检测到八个miRNA let-7家族成员，对miRNA let-7a的特异性低至0.2 pM，0.8 pM，0.2 pM，0.2 pM，0.2 pM，0.8 pM，0.8 pM和0.8 pM。分别为7g和7i。此外，已评估了来自五个细胞系的总RNA提取物中八个miRNA let-7家族成员的表达水平，并获得了令人满意的结果。