SiO₂ nanospheres were synthesized by the sol–gel method using tetraethoxysilane (TEOS) as the precursor, which was then modified with 3-aminopropyltriethoxysilane (APTES) for the grafting of NH₂ to the SiO₂ surface. Au nanoparticles (AuNPs) were anchored to the NH₂ grafted SiO₂, and then l-cysteine (L-Cys) templates were introduced to the SiO₂/AuNPs through forming AuS bond. Another layer of SiO₂ was then coated on the L-Cys doped SiO₂/AuNPs as the coating layer, and then the templates were removed by calcination to form a sandwich-structured molecularly imprinted SiO₂/AuNPs/SiO₂. Finally, the molecularly imprinted chiral interface was applied for electrochemical enantioselective recognition of Cys isomers, and discernible differences in the current signals could be observed on the differential pulse voltammograms (I_D-cys/I_L-cys, 2.36).

SiO ₂纳米球是通过溶胶-凝胶法合成的，使用四乙氧基硅烷（TEOS）作为前体，然后用3-氨丙基三乙氧基硅烷（APTES）进行改性，以将NH ₂接枝到SiO ₂表面。将Au纳米颗粒（AuNPs）固定在接枝了NH _2的SiO _2上，然后通过形成Au S键将1-半胱氨酸（L-Cys）模板引入SiO ₂ / AuNPs中。然后将另一层SiO ₂涂覆在掺杂L-Cys的SiO ₂ / AuNPs上作为涂层，然后通过煅烧去除模板以形成夹心结构的分子印迹SiO₂ / AuNPs / SiO ₂。最后，将分子印迹手性界面用于Cys异构体的电化学对映选择性识别，并且在差分脉冲伏安图上可以观察到电流信号的明显差异（I _D-cys / I _L-cys，2.36）。