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Effects of pristine polyvinyl chloride fragments on whole body histology and protease activity in silver barb Barbodes gonionotus fry
Environmental Pollution ( IF 8.9 ) Pub Date : 2017-11-20 , DOI: 10.1016/j.envpol.2017.11.040
Nicholas Romano , Munirah Ashikin , Jun Chin Teh , Fadhil Syukri , Ali Karami

Silver barb Barbodes gonionotus fry were exposed to polyvinyl chloride (PVC) fragments at increasing concentrations of 0.2, 0.5 and 1.0 mg/L for 96 h, following which whole body histological evaluation and analysis of the digestive enzymes trypsin and chymotrypsin were performed. Whole body trypsin and chymotrypsin activities increased significantly in fish exposed to 0.5 and 1.0 mg/L PVC as compared those exposed to zero or 0.2 mg/L PVC. In fish exposed to all tested concentrations, PVCs were observed in both the proximal and distal intestine, and fish exposed to 0.5–1.0 and 1.0 mg/L PVC, respectively, and these particles were associated with localized thickening of the mucosal epithelium. No tissue damage was evident in any other internal organs or gills. This lack of damage may be attributed to the absence of contaminants associated with the PVC fragments and their relatively smooth surface. The increased whole body trypsin and chymotrypsin activities may indicate an attempt to enhance digestion to compensate for epithelial thickening of the intestine and/or to digest the plastics.



中文翻译:

原始聚氯乙烯片段对银倒刺Barbodes gonionotus鱼苗的全身组织学和蛋白酶活性的影响

银倒钩Barbodes gonionotus将鱼苗暴露于浓度分别为0.2、0.5和1.0 mg / L的聚氯乙烯(PVC)片段中96小时,然后进行全身组织学评估和消化酶胰蛋白酶和胰凝乳蛋白酶的分析。与暴露于零或0.2 mg / L PVC的鱼相比,暴露于0.5和1.0 mg / L PVC的鱼的全身胰蛋白酶和胰凝乳蛋白酶活性显着增加。在暴露于所有测试浓度的鱼中,在近端和远端的肠道中均观察到PVC,而暴露于0.5-1.0和1.0 mg / L PVC的鱼中也观察到PVC,这些颗粒与粘膜上皮的局部增厚有关。在其他任何内部器官或g中均未见明显的组织损伤。损坏的缺乏可能归因于不存在与PVC碎片及其相对光滑的表面相关的污染物。全身胰蛋白酶和胰凝乳蛋白酶活性的增加可能表明尝试增强消化以补偿肠上皮增厚和/或消化塑料。

更新日期:2017-11-20
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