Lung cancer is the leading cause of cancer-related deaths worldwide, and approximately 85% are diagnosed as non-small-cell lung cancer (NSCLC). However, efficient detection and diagnosis of NSCLC at early stage is still challenging. In this work, we developed a simple, ultrasensitive and high selective strategy for A549 human NSCLC cells detection based on combining the reorganization property of a novel cyanine dye 3,3′-di(3-sulfopropyl)-4,5,4′,5′-dibenzo-9-methyl-thiacarbocyanine triethylammonium salt (cy-M) to aptamer S6 G-quadruplex structure having specific affinity to NSCLC cells, which induced a dramatic fluorescence enhancement (~ 10⁴ times). Moreover, this strategy was successfully used for detecting of A549 cancer cells in complex media such as human serum and clinical pleural effusion, which strong indicated that the proposed method could be applied for the early diagnosis of lung cancer.

肺癌是世界范围内与癌症相关的死亡的主要原因，大约有85％被诊断为非小细胞肺癌（NSCLC）。但是，在早期有效检测和诊断NSCLC仍具有挑战性。在这项工作中，我们结合了新型花青染料3,3'-di（3-磺丙基）-4,5,4'的重组特性，为A549人NSCLC细胞检测开发了一种简单，超灵敏，高选择性的策略，与适体S6 G-四链体结构具有对NSCLC细胞具有特异性亲和力的5'-二苯并-9-甲基-硫代羰基花青三乙铵盐（cy-M）（〜10 ⁴次）。此外，该策略已成功用于检测人血清和临床胸腔积液等复杂培养基中的A549癌细胞，这强烈表明该方法可用于肺癌的早期诊断。