High-efficient enrichment of glycopeptides prior to mass spectrometry is essential for glycoproteomics analysis. Hydrophilic interaction chromatography (HILIC) approach is a prominent strategy for glycopeptides identification. In this work, glucose functionalized magnetic graphene hydrophilic nanocomposite (MagG/Au/Glu) was synthesized as a novel HILIC material via a facile surface modification strategy. Different from previous click synthesis of saccharides-functionalized materials, glucose was easily thiolated via Traut's reagent and then immobilized on the materials via efficient Au-S coupling, greatly simplifying the synthesis process. Combining the rapid magnetic response, huge surface area from graphene and excellent hydrophilicity from glucose, MagG/Au/Glu nanocomposites afforded convenience of the operation and affinity for glycopeptides. Thus, the nanocomposites exhibited superior performance of high sensitivity, selectivity and reusability in glycopeptide enrichment from tryptic digests of standard glycoprotein HRP. Encouragingly, with the usage of MagG/Au/Glu nanocomposites, a total of 305 glycopeptides assigned to 108 glycoproteins were identified from complex real sample human serum, indicating a great potential for the application of glycoproteomics research.

质谱之前高效富集糖肽对于糖蛋白组学分析至关重要。亲水相互作用色谱法（HILIC）是糖肽鉴定的重要策略。在这项工作中，通过一种简便的表面修饰策略，将葡萄糖官能化的磁性石墨烯亲水性纳米复合材料（MagG / Au / Glu）合成为新型HILIC材料。与以前的糖功能化材料的点击合成不同，葡萄糖易于通过Traut's试剂进行硫醇化，然后通过有效的Au-S偶联固定在材料上，从而大大简化了合成过程。结合了快速的磁响应，石墨烯的巨大表面积和葡萄糖的出色亲水性，MagG / Au / Glu纳米复合材料提供了操作方便和对糖肽的亲和力。因此，在从标准糖蛋白HRP的胰蛋白酶消化物中富集糖肽时，纳米复合材料表现出了高灵敏度，选择性和可重复使用性的优异性能。令人鼓舞的是，通过使用MagG / Au / Glu纳米复合材料，从复杂的真实样品人血清中鉴定出总共305种糖肽，分配给108种糖蛋白，这表明糖蛋白组学研究的巨大潜力。